Label-free detection of cancer biomarker candidates using surface plasmon resonance imaging

2008 ◽  
Vol 393 (4) ◽  
pp. 1157-1163 ◽  
Author(s):  
Jon Ladd ◽  
Allen D. Taylor ◽  
Marek Piliarik ◽  
Jiri Homola ◽  
Shaoyi Jiang
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Cancer Biomarker ◽  
Surface Plasmon Resonance Imaging ◽  
Label Free ◽  
Resonance Imaging ◽  
Label Free Detection

Label-free detection of Alzheimer’s disease through the ADP3 peptoid recognizing the serum amyloid-beta42 peptide

2015 ◽  
Vol 51 (4) ◽  
pp. 718-721 ◽  
Author(s):  
Zijian Zhao ◽  
Ling Zhu ◽  
Xiangli Bu ◽  
Huailei Ma ◽  
Shu Yang ◽  
...  
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Serum Amyloid ◽  
Surface Plasmon Resonance Imaging ◽  
Label Free ◽  
Resonance Imaging ◽  
Label Free Detection

Surface plasmon resonance imaging in combination with the ADP3 peptoid was used to identify Alzheimer’s disease through detecting amyloid-beta42 in the serum.

Rapid label-free detection of intact pathogenic bacteria in situ via surface plasmon resonance imaging enabled by crossed surface relief gratings

The Analyst ◽  
2020 ◽  
Vol 145 (6) ◽  
pp. 2133-2142 ◽  
Author(s):  
Srijit Nair ◽  
Juan Gomez-Cruz ◽  
Ángel Manjarrez-Hernandez ◽  
Gabriel Ascanio ◽  
Ribal Georges Sabat ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Surface Relief ◽  
Pathogenic Bacteria ◽  
Surface Plasmon Resonance Imaging ◽  
Label Free ◽  
Resonance Imaging ◽  
Label Free Detection ◽  
Surface Relief Gratings

The unique plasmonic energy exchange occurring within metallic crossed surface relief gratings (CSRGs) enables Surface Plasmon Resonance Imaging for the label-free detection of whole uropathogenic bacteria.

LABEL-FREE DETECTION OF PROTEIN MICROARRAY WITH HIGH THROUGHPUT SURFACE PLASMON RESONANCE IMAGING (SPRI)

2008 ◽  
Vol 01 (01) ◽  
pp. 107-114 ◽  
Author(s):  
SHUKUAN XU ◽  
GUOLIANG HUANG ◽  
CHENG DENG ◽  
JIANG ZHU ◽  
CHAO HAN ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
High Throughput ◽  
Plasmon Resonance ◽  
Incident Angle ◽  
Protein Microarray ◽  
Surface Plasmon Resonance Imaging ◽  
Label Free ◽  
Resonance Imaging ◽  
Label Free Detection

A surface plasmon resonance imaging (SPRI) system was developed for the discrimination of proteins on a gold surface. As a label-free and high-throughput technique, SPRI enables simultaneously monitoring of the biomolecular interactions at low concentrations. We used SPRI as a label-free and parallel method to detect different proteins based on protein microarray. Bovine Serum Albumin (BSA), Casein and Immunoglobulin G (IgG) were immobilized onto the Au surface of a gold-coated glass chip as spots forming a 6 × 6 matrix. These proteins can be discriminated directly by changing the incident angle of light. Excellent reproducibility for label-free detection of protein molecules was achieved. This SPRI platform represents a simple and robust method for performing high-sensitivity detection of protein microarray.

scholarly journals The determination of cystatin C in biological samples via the surface plasmon resonance method

BioTechniques ◽  
2021 ◽  
Author(s):  
Michal Lesnak ◽  
Dominik Jursa ◽  
Marek Miskay ◽  
Helena Riedlova ◽  
Karla Barcova ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Cystatin C ◽  
Plasmon Resonance ◽  
Repeated Measurements ◽  
Imaging Method ◽  
Surface Plasmon Resonance Imaging ◽  
Label Free ◽  
Resonance Imaging ◽  
Label Free Detection

Surface plasmon resonance imaging biosensors have a number of advantages that make them superior to other analytical methods. These include the possibility of label-free detection, speed and high sensitivity to low protein concentrations. The aim of this study was to create and analyze biochips, with the help of which it is possible to test cystatin C in patient urine samples and compare the results with the one-time traditional ELISA method. The main advantage of the surface plasmon resonance imaging method is the possibility of repeated measurements over a long period of time in accordance with clinical practice. The surface of the biochip was spotted with anticystatin C and a negative control of mouse IgG at a ratio of 1:1. The aforementioned biochip was first verified using standard tests and then with patient samples, which clearly confirmed the required sensitivity even for very low concentrations of cystatin C.

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