Development and validation of a multiplex real-time PCR method to simultaneously detect 47 targets for the identification of genetically modified organisms

2013 ◽  
Vol 405 (21) ◽  
pp. 6831-6844 ◽  
Author(s):  
Geoffrey Cottenet ◽  
Carine Blancpain ◽  
Véronique Sonnard ◽  
Poh Fong Chuah
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Genetically Modified Organisms ◽  
Genetically Modified ◽  
Pcr Method ◽  
Development And Validation

scholarly journals Evaluation of a Real Time PCR Assay Method for the Detection of Genetically Modified Organisms in Food Products

2020 ◽  
Vol 9 (2) ◽  
pp. 1
Author(s):  
Eleni Spanea ◽  
Theofania Tsironi ◽  
Efstathia Tsakali ◽  
Anthimia Batrinou ◽  
Valentina Stefanou ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Genetically Modified Organisms ◽  
Genetically Modified ◽  
Food Products ◽  
Assay Method ◽  
Genetically Modified Maize ◽  
Nos Terminator ◽  
Repeatability And Reproducibility ◽  
Pcr Method

The objective of the study was to determine qualitatively by validated Real Time PCR method the occurrence of genetically modified maize and soybean in commercial food products from the Greek market. 70 independent samples were collected, including products from different categories (i.e. cereal based, biscuits and snacks) which declared either corn or soybean on the labelling. The result of the study indicated that 37.1% of maize and soy products (n=70) displayed in the Greek market have detectable levels of genetically modified maize or soy. These products were identified by specific primers and included common GMΟ detection primers for 35S and NOS terminator. Adequate repeatability and reproducibility was demonstrated for the applied Real Time PCR method, as evaluated by intra- and inter-laboratory tests.

scholarly journals Development and validation of real-time PCR screening methods for detection of cry1A.105 and cry2Ab2 genes in genetically modified organisms

2011 ◽  
Vol 400 (5) ◽  
pp. 1433-1442 ◽  
Author(s):  
Andréia Z. Dinon ◽  
Theo W. Prins ◽  
Jeroen P. van Dijk ◽  
Ana Carolina M. Arisi ◽  
Ingrid M. J. Scholtens ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Genetically Modified Organisms ◽  
Genetically Modified ◽  
Screening Methods ◽  
Pcr Screening ◽  
Development And Validation

Two FAST multiplex real-time PCR reactions to assess the presence of genetically modified organisms in food

2019 ◽  
Vol 274 ◽  
pp. 760-765 ◽  
Author(s):  
Geoffrey Cottenet ◽  
Carine Blancpain ◽  
Véronique Sonnard ◽  
Poh Fong Chuah
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Genetically Modified Organisms ◽  
Genetically Modified

scholarly journals Verification of Real-Time PCR Methods for Qualitative and Quantitative Testing of Genetically Modified Organisms

2012 ◽  
Vol 35 (6) ◽  
pp. 442-447 ◽  
Author(s):  
S. Del Gaudio ◽  
A. Cirillo ◽  
G. Di Bernardo ◽  
U. Galderisi ◽  
M. Cipollaro
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Genetically Modified Organisms ◽  
Genetically Modified ◽  
Qualitative And Quantitative
Sign in / Sign up

Export Citation Format

Share Document