Potential Use of Colored LED Lights to Increase the Production of Bioactive Metabolites Hedyotis corymbosa (L.) Lam

Hedyotis corymbosa (L.) Lam is a wild herb that is used in traditional Indian, Chinese, and African medicine. Light-emitting diode (LED) technology is paving the way to enhance crop production and inducing targeted photomorphogenic, biochemical, or physiological responses in plants. This study examines the efficiency of H. corymbosa (L.) Lam production under blue 450 nm and red 660 nm LED lights for overall plant growth, photosynthetic characteristics, and the contents of metabolite compounds. Our research showed that blue LED lights provided a positive effect on enhancing plant growth and overall biomass. In addition, blue LED lights are more effective in controlling the production of sucrose, starch, total phenolic compounds, and total flavonoid compared to red LED lights. However, blue and red LED lights played essential but different roles in photosynthetic characteristics. Our results showed the potential of colored LED light applications in improving farming methods and increasing metabolite production in herbs. LED lights are safer alternatives than genetically modified organisms or genome editing.

Continuous Production of Fumaric Acid with Immobilised Rhizopus oryzae: The Role of pH and Urea Addition

Fumaric acid is widely used in the food and beverage, pharmaceutical and polyester resin industries. Rhizopus oryzae is the most successful microorganism at excreting fumaric acid compared to all known natural and genetically modified organisms. It has previously been discovered that careful control of the glucose feed rate can eliminate the by-product formation of ethanol. Two key parameters affecting fumaric acid excretion were identified, namely the medium pH and the urea feed rate. A continuous fermentation with immobilised R. oryzae was utilised to determine the effect of these parameters. It was found that the selectivity for fumaric acid production increased at high glucose consumption rates for a pH of 4, different from the trend for pH 5 and 6, achieving a yield of 0.93 gg−1. This yield is higher than previously reported in the literature. Varying the urea feed rate to 0.255 mgL−1h−1 improved the yield of fumaric acid but experienced a lower glucose uptake rate compared to higher urea feed rates. An optimum region has been found for fumaric acid production at pH 4, a urea feed rate of 0.625 mgL−1h−1 and a glucose feed rate of 0.329 gL−1h−1.

Analysis of feed in terms of the presence of genetically modified oilseed rape

The labeling of products containing GMOs above 0.9% results from the provisions of the law in force in the European Union. The aim of the study was to evaluate the presence of genetically modified rape in feed used in feeding farm animals in Poland. The research was based on the real-time PCR technique, using primers and event-specific probes. A total of 900 samples were analyzed. The research identified canola line GT73, resistant to glyphosate. Only in 12 samples did the content of this oilseed rape exceed the threshold above which the product must be labeled as containing GMOs. Conducting such controls is extremely important because of the possibility of contamination of feed free from genetically modified organisms, which are increasingly used in animal nutrition.

Determination of the Detection Limit of the Detection of GMO in Food Using the Isothermal Solid-Phase Recombinase Polymerase Amplification on Microfluidic DVDs

A major drawback of the current literature on bioassay development is that these tests are not made using statistically robust methods for establishing the limit of detection. As an alternative, researchers often make use of simple detection-limit methods that are only roughly indicative of the actual detection limit. We can only assume that this is due to a practical need for simplified processes, in addition to the notion that the limit of detection theory has already been lowered to practice for bioassays. A DNA sensor based on light intensity of the scanning laser on a DVD drive with microfluidic layer etched onto the polycarbonate surface of an ordinary DVD has been previously developed for fast screening of genetically modified organisms (GMOs). The resultant calibration curve showed a sigmoidal calibration curve but was not modelled according to any of the sigmoidal models available. The objective of this study is the remodel the data using the standard 4-PL model and to determine the Limits of Detection (LOD) based on the standard method. The LOD value obtained through the 4PL modelling exercise based on a pooled standard deviation method yielded an LOD value of 62 mg/g (95% confidence interval of 17 to 158), which was quite similar to the classical three standard deviation of the blank method but was lower than the rough estimation employed in the original publication.

Comparative Characteristics of the Amino Acids Composition of Sheep Milk from Breeds of Northern Kazakhstan

Kazakhstan is characterized by a wide variety of natural, climatic, economic, and ethnic factors that have a specific impact on the development of sheep farming. The production of dairy products from sheep is limited due to the low productivity of animals and the seasonality of milk production throughout the year, as well as the short lactation period. Also, one of the problems is manual milking of sheep, which does not allow obtaining sheep milk on an industrial scale. The market of dairy products, currently in the Republic of Kazakhstan is represented only by-products made from cow's milk, and at the same time, most of them are products made from cow's milk powder, so the development of products made from sheep's milk is an urgent task. In addition, sheep's milk is a national product, and one of the tasks is to revive the traditional products of the Kazakh people. The analysis of literature studies shows that in-depth studies of the chemical composition of sheep's milk have not been conducted in Kazakhstan. The chemical composition data obtained during the Soviet period only give an idea of the primary chemical parameters obtained by generally accepted basic methods and do not provide reliable information about the deep chemical composition of the milk of local ewes. According to the results of studies, the content of the essential amino acid valine in cow's milk (0.21 g/100g) is also lower than in sheep's milk (9.69-3.79 g/100g). Sheep's milk protein is digested in the human body by 99.1%, and cow's milk protein by 91.9%. According to world experts, one of the advantages of Kazakhstan's domestic milk products is its naturalness, a low share of the use of genetically modified organisms, and environmentally friendly pastures, due to which they receive high-quality and safe products. Therefore, the designated research topic and its analysis are appropriate and relevant.