Development and Validation of Duplex, Triplex, and Pentaplex Real-Time PCR Screening Assays for the Detection of Genetically Modified Organisms in Food and Feed

2013 ◽  
Vol 61 (43) ◽  
pp. 10293-10301 ◽  
Author(s):  
Ingrid Huber ◽  
Annette Block ◽  
Daniela Sebah ◽  
Frédéric Debode ◽  
Dany Morisset ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Genetically Modified Organisms ◽  
Genetically Modified ◽  
Pcr Screening ◽  
Food And Feed ◽  
Screening Assays ◽  
Development And Validation

scholarly journals Development and validation of real-time PCR screening methods for detection of cry1A.105 and cry2Ab2 genes in genetically modified organisms

2011 ◽  
Vol 400 (5) ◽  
pp. 1433-1442 ◽  
Author(s):  
Andréia Z. Dinon ◽  
Theo W. Prins ◽  
Jeroen P. van Dijk ◽  
Ana Carolina M. Arisi ◽  
Ingrid M. J. Scholtens ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Genetically Modified Organisms ◽  
Genetically Modified ◽  
Screening Methods ◽  
Pcr Screening ◽  
Development And Validation

Two FAST multiplex real-time PCR reactions to assess the presence of genetically modified organisms in food

2019 ◽  
Vol 274 ◽  
pp. 760-765 ◽  
Author(s):  
Geoffrey Cottenet ◽  
Carine Blancpain ◽  
Véronique Sonnard ◽  
Poh Fong Chuah
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Genetically Modified Organisms ◽  
Genetically Modified

scholarly journals Verification of Real-Time PCR Methods for Qualitative and Quantitative Testing of Genetically Modified Organisms

2012 ◽  
Vol 35 (6) ◽  
pp. 442-447 ◽  
Author(s):  
S. Del Gaudio ◽  
A. Cirillo ◽  
G. Di Bernardo ◽  
U. Galderisi ◽  
M. Cipollaro
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Genetically Modified Organisms ◽  
Genetically Modified ◽  
Qualitative And Quantitative

scholarly journals Evaluation of a Real Time PCR Assay Method for the Detection of Genetically Modified Organisms in Food Products

2020 ◽  
Vol 9 (2) ◽  
pp. 1
Author(s):  
Eleni Spanea ◽  
Theofania Tsironi ◽  
Efstathia Tsakali ◽  
Anthimia Batrinou ◽  
Valentina Stefanou ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Genetically Modified Organisms ◽  
Genetically Modified ◽  
Food Products ◽  
Assay Method ◽  
Genetically Modified Maize ◽  
Nos Terminator ◽  
Repeatability And Reproducibility ◽  
Pcr Method

The objective of the study was to determine qualitatively by validated Real Time PCR method the occurrence of genetically modified maize and soybean in commercial food products from the Greek market. 70 independent samples were collected, including products from different categories (i.e. cereal based, biscuits and snacks) which declared either corn or soybean on the labelling. The result of the study indicated that 37.1% of maize and soy products (n=70) displayed in the Greek market have detectable levels of genetically modified maize or soy. These products were identified by specific primers and included common GMΟ detection primers for 35S and NOS terminator. Adequate repeatability and reproducibility was demonstrated for the applied Real Time PCR method, as evaluated by intra- and inter-laboratory tests.

Evaluation of Systems for Nopaline Synthase Terminator in Fast and Standard Real-Time PCR to Screen Genetically Modified Organisms

2015 ◽  
Vol 9 (4) ◽  
pp. 1009-1019 ◽  
Author(s):  
Elisa Pierboni ◽  
Ludovica Curcio ◽  
Gloria Raquel Tovo ◽  
Martina Torricelli ◽  
Cristina Rondini
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Genetically Modified Organisms ◽  
Genetically Modified

Combinatory SYBR® Green Real-Time PCR Screening Approach for Tracing Materials Derived from Genetically Modified Rice

2012 ◽  
Vol 6 (2) ◽  
pp. 361-369 ◽  
Author(s):  
L. Kluga ◽  
S. Folloni ◽  
D. M. Kagkli ◽  
A. Bogni ◽  
N. Foti ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Genetically Modified ◽  
Genetically Modified Rice ◽  
Pcr Screening ◽  
Screening Approach
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