Isolation and determination of dissolved organic sulphur compounds — development of the organic group parameter DOS

1997 ◽  
Vol 357 (4) ◽  
pp. 411-415 ◽  
Author(s):  
F. Binde ◽  
H. -H. Rüttinger
Keyword(s):  
Sulphur Compounds ◽  
Organic Group ◽  
Organic Sulphur ◽  
Group Parameter

Determination of organic sulphur compounds in garlic extracts by gas chromatography and mass spectrometry

1995 ◽  
Vol 53 (1) ◽  
pp. 91-93 ◽  
Author(s):  
R.Artacho Martín-Lagos ◽  
M.F.Olea Serrano ◽  
M.D.Ruiz Lopez
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Sulphur Compounds ◽  
Organic Sulphur

scholarly journals An HPLC-automated Derivatization for Glutathione and Related Thiols Analysis in Brassica rapa L.

Agronomy ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1157
Author(s):  
Francesco Nacca ◽  
Concetta Cozzolino ◽  
Petronia Carillo ◽  
Pasqualina Woodrow ◽  
Amodio Fuggi ◽  
...  
Keyword(s):  
Brassica Rapa ◽  
High Performance ◽  
Internal Standard ◽  
Plant Tissues ◽  
Cellular Functions ◽  
Sulphur Compounds ◽  
Stress Protection ◽  
Automated Method ◽  
Reduced Forms

The high content of glucosinolates and glutathione makes the Brassicaceae an important healthy food. Thiols and especially glutathione and γ-Glu-Cys-Gly tripeptide are involved in many fundamental cellular functions such as oxidative stress protection. Although several methods for sulphur compounds analysis in biological samples are actually used, the determination of glutathione and other sulphur derivatives in plant tissues is rather problematic due to their extreme susceptibility to oxidation, which can lead to their overestimation. The aim of this work was the improvement and validation of an automated method for determination of reduced and oxidised glutathione, cysteine and γ-glutamylcysteine in plant tissues. The method consists of a fully automated pre-column derivatization of thiols based on monobromobimane reagent, a high-performance liquid chromatography derivatives separation, and a fluorimetric detection and quantification. The method was successfully applied for determination of the oxidized and reduced forms of Cys, γ-GC and GSH content in leaves, petioles, inflorescences and roots of Brassica rapa L. subsp. Sylvestris. At harvest, in freshly cut plants, the average contents of GSH/2GSSG were 840/45, 345/70 and 150/70 nmol g−1 FW for the florets, leaf blades and stems, respectively; those of Cys/2Cys were 80/12, 29/12 and 24/6 nmol g-1 FW; while those of γ-GC/γ-GCCG-γ were 8.0/4.0, and 6.0/3.0, 3.0/2.0 nmol g−1 FW, respectively. Such amounts were lower in low-sulphur-grown plants at harvest. The very low coefficient of variation between repeated tests (maximum 1.6%), the high recovery of internal standard (>96%) and the linear correlation coefficient of the calibration (R2 > 0.99) support the efficiency of this method that allowed analysing about 50 samples/die in a totally automated manner with no operator intervention. Our results show that the reported method integrations can significantly improve thiols detection via HPLC.

scholarly journals Stud es on the Organic Sulphur Compounds. VII

1944 ◽  
Vol 65 (7) ◽  
pp. 650-654
Author(s):  
Kiuji ABE
Keyword(s):  
Sulphur Compounds ◽  
Organic Sulphur

Reactions of the nitrate radical with a series of reduced organic sulphur compounds in air

1992 ◽  
Vol 24 (10) ◽  
pp. 839-850 ◽  
Author(s):  
N. R. Jensen ◽  
J. Hjorth ◽  
C. Lohse ◽  
H. Skov ◽  
G. Restelli
Keyword(s):  
Nitrate Radical ◽  
Sulphur Compounds ◽  
Organic Sulphur
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