Quantitative changes in gene expression of glutamate receptor subunits/subtypes in the vestibular nucleus, inferior olive and flocculus before and following unilateral labyrinthectomy in the rat: real-time quantitative PCR method

2001 ◽  
Vol 139 (2) ◽  
pp. 188-200 ◽  
Author(s):  
Arata Horii ◽  
Paul Smith ◽  
Cynthia Darlington
Keyword(s):  
Gene Expression ◽  
Real Time ◽  
Quantitative Pcr ◽  
Glutamate Receptor ◽  
Inferior Olive ◽  
Vestibular Nucleus ◽  
Real Time Quantitative Pcr ◽  
Unilateral Labyrinthectomy ◽  
Pcr Method ◽  
Quantitative Changes

Human disease characterization: real-time quantitative PCR analysis of gene expression

2001 ◽  
Vol 6 (20) ◽  
pp. 1062-1067 ◽  
Author(s):  
James V Snider ◽  
Mark A Wechser ◽  
Izidore S Lossos
Keyword(s):  
Gene Expression ◽  
Real Time ◽  
Quantitative Pcr ◽  
Human Disease ◽  
Pcr Analysis ◽  
Real Time Quantitative Pcr

scholarly journals Development of a Real-Time qPCR Method for Detection and Enumeration of Mycobacterium spp. in Surface Water

2010 ◽  
Vol 76 (21) ◽  
pp. 7348-7351 ◽  
Author(s):  
Nicolas Radomski ◽  
Françoise S. Lucas ◽  
Régis Moilleron ◽  
Emmanuelle Cambau ◽  
Sophie Haenn ◽  
...  
Keyword(s):  
Surface Water ◽  
16S Rrna ◽  
Real Time ◽  
Quantitative Pcr ◽  
Environmental Samples ◽  
Real Time Quantitative Pcr ◽  
Qpcr Method ◽  
Pcr Method ◽  
Real Time Qpcr ◽  
Mycobacterium Spp

ABSTRACT A real-time quantitative PCR method was developed for the detection and enumeration of Mycobacterium spp. from environmental samples and was compared to two other methods already described. The results showed that our method, targeting 16S rRNA, was more specific than the two previously published real-time quantitative PCR methods targeting another 16S rRNA locus and the hsp65 gene (100% versus 44% and 91%, respectively).

Validation of reference genes for accurate normalization of gene expression for real time-quantitative PCR in strawberry fruits using different cultivars and osmotic stresses

Gene ◽  
2015 ◽  
Vol 554 (2) ◽  
pp. 205-214 ◽  
Author(s):  
Vanessa Galli ◽  
Joyce Moura Borowski ◽  
Ellen Cristina Perin ◽  
Rafael da Silva Messias ◽  
Julia Labonde ◽  
...  
Keyword(s):  
Gene Expression ◽  
Real Time ◽  
Quantitative Pcr ◽  
Reference Genes ◽  
Real Time Quantitative Pcr ◽  
Accurate Normalization

scholarly journals Establishing reference genes for use in real-time quantitative PCR analysis of early equine embryos

2011 ◽  
Vol 23 (2) ◽  
pp. 353 ◽  
Author(s):  
Damien B. B. P. Paris ◽  
Ewart W. Kuijk ◽  
Bernard A. J. Roelen ◽  
Tom A. E. Stout
Keyword(s):  
Gene Expression ◽  
Real Time ◽  
Quantitative Pcr ◽  
Embryo Development ◽  
Reference Genes ◽  
Stable Expression ◽  
Pcr Analysis ◽  
Blastocyst Development ◽  
Real Time Quantitative Pcr

Real-time quantitative PCR (qPCR) is invaluable for investigating changes in gene expression during early development, since it can be performed on the limited quantities of mRNA contained in individual embryos. However, the reliability of this method depends on the use of validated stably expressed reference genes for accurate data normalisation. The aim of the present study was to identify and validate a set of reference genes suitable for studying gene expression during equine embryo development. The stable expression of four carefully selected reference genes and one developmentally regulated gene was examined by qPCR in equine in vivo embryos from morula to expanded blastocyst stage. SRP14, RPL4 and PGK1 were identified by geNorm analysis as stably expressed reference genes suitable for data normalisation. RPL13A expression was less stable and changed significantly during the period of development examined, rendering it unsuitable as a reference gene. As anticipated, CDX2 expression increased significantly during embryo development, supporting its possible role in trophectoderm specification in the horse. In summary, it was demonstrated that evidence-based selection of potential reference genes can reduce the number needed to validate stable expression in an experimental system; this is particularly useful when dealing with tissues that yield small amounts of mRNA. SRP14, RPL4 and PGK1 are stable reference genes suitable for normalising expression for genes of interest during in vivo morula to expanded blastocyst development of horse embryos.

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