The macrolide antibiotic azithromycin interacts with lipids and affects membrane organization and fluidity: Studies on langmuir-blodgett monolayers, liposomes and J774 macrophages

2003 ◽  
Vol 192 (3) ◽  
pp. 203-215 ◽  
Author(s):  
D. Tyteca ◽  
A. Schanck ◽  
Y. F. Dufrêne ◽  
M. Deleu ◽  
P. J. Courtoy ◽  
...  
Keyword(s):  
Macrolide Antibiotic ◽  
Membrane Organization ◽  
Langmuir Blodgett

scholarly journals Interaction of the Macrolide Antibiotic Azithromycin with Lipid Bilayers: Effect on Membrane Organization, Fluidity, and Permeability

2005 ◽  
Vol 22 (3) ◽  
pp. 465-475 ◽  
Author(s):  
A. Berquand ◽  
N. Fa ◽  
Y. F. Dufr�ne ◽  
M.-P. Mingeot-Leclercq
Keyword(s):  
Lipid Bilayers ◽  
Macrolide Antibiotic ◽  
Membrane Organization

Ultrastructural autoradiography of L6 skeletal-muscle cells exposed to a tritiated macrolide antibiotic (LY237216) in vitro

1992 ◽  
Vol 50 (1) ◽  
pp. 612-613
Author(s):  
S.L. White ◽  
C.B. Jensen ◽  
D.D. Giera ◽  
D.A. Laska ◽  
M.N. Novilla ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Quantitative Analysis ◽  
Macrolide Antibiotic ◽  
Circle Method ◽  
Apical Surface ◽  
Polycarbonate Membrane ◽  
L6 Cells ◽  
Low Viscosity ◽  
Erythromycin A

In vitro exposure to LY237216 (9-Deoxo-11-deoxy-9,11-{imino[2-(2-methoxyethoxy)ethylidene]-oxy}-(9S)-erythromycin), a macrolide antibiotic, was found to induce cytoplasmic vacuolation in L6 skeletal muscle myoblast cultures (White, S.L., unpubl). The present study was done to determine, by autoradiographic quantitative analysis, the subcellular distribution of 3H-LY237216 in L6 cells.L6 cells (ATCC, CRL 1458) were cultured to confluency on polycarbonate membrane filters (Millipore Corp., Bedford, MA) in M-199 medium (GIBCO® Labs) with 10% fetal bovine serum. The cells were exposed from the apical surface for 1-hour to unlabelled-compound (0 μCi/ml) or 50 (μCi/ml of 3H-LY237216 at a compound concentration of 0.25 mg/ml. Following a rapid rinse in compound-free growth medium, the cells were slam-frozen against a liquid nitrogen cooled, polished copper block in a CF-100 cryofixation unit (LifeCell Corp., The Woodlands, TX). Specimens were dried in the MDD-C Molecular Distillation Drier (LifeCell Corp.), vapor osmicated and embedded in Spurrs low viscosity resin. Ultrathin sections collected on formvar coated stainless steel grids were counter-stained, then individually mounted on corks. A monolayer of Ilford L4 nuclear emulsion (Polysciences, Inc., Warrington, PA) was placed on the sections, utilizing a modified “loop method”. The emulsions were exposed for 7-weeks in a light-tight box at 4°C. Autoradiographs were developed in Microdol-X developer and examined on a Philips EM410LS transmission electron microscope. Quantitative analysis of compound localization employed the point and circle approach of Williams; incorporating the probability circle method of Salpeter and McHenry.

New Amphiphilic Tetrathiafulvalene (TTF) Derivatives: Synthesis and Langmuir-Blodgett Filn Formation

1988 ◽  
Vol 156 (1) ◽  
pp. 339-345 ◽  
Author(s):  
FranÇOise Bertho ◽  
Daniel Talham ◽  
Albert Robert ◽  
Patrick Batail ◽  
Stephane Megtert ◽  
...  
Keyword(s):  
Langmuir Blodgett

Langmuir-blodgett films of liquid-crystalline heteroaromatic cations associated with diamagnetic (BF4-, CIO4-) or paramagnetic (TCNQ[math]) anions

1988 ◽  
Vol 85 ◽  
pp. 385-389 ◽  
Author(s):  
M. Vandevyver ◽  
J. Richard ◽  
A Barraud ◽  
M. Veber ◽  
C Jallabert ◽  
...  
Keyword(s):  
Liquid Crystalline ◽  
Langmuir Blodgett ◽  
Langmuir Blodgett Films

Non linear physical properties of langmuir-blodgett films : their possible applications

1988 ◽  
Vol 85 ◽  
pp. 1093-1097 ◽  
Author(s):  
G.G. Roberts ◽  
B. Holcroft ◽  
T. Richardson ◽  
R. Colbrook
Keyword(s):  
Physical Properties ◽  
Langmuir Blodgett ◽  
Non Linear ◽  
Langmuir Blodgett Films

The reproducibly perfect langmuir-blodgett film

1988 ◽  
Vol 85 ◽  
pp. 997-1001 ◽  
Author(s):  
I.R. Peterson
Keyword(s):  
Blodgett Film ◽  
Langmuir Blodgett ◽  
Langmuir Blodgett Film

Étude par spectroscopie infrarouge de la structure de couches de Langmuir-Blodgett

1979 ◽  
Vol 76 ◽  
pp. 909-917 ◽  
Author(s):  
Claudine Vogel ◽  
Jacques Corset ◽  
Monique Dupeyrat
Keyword(s):  
Langmuir Blodgett
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