Identification of MHC class I sequences in Chinese-origin rhesus macaques

ABSTRACT Certain major histocompatibility complex (MHC) class I alleles are associated with the control of human immunodeficiency virus and simian immunodeficiency virus (SIV) replication. We have designed sequence-specific primers for detection of the rhesus macaque MHC class I allele Mamu-B*08 by PCR and screened a cohort of SIV-infected macaques for this allele. Analysis of 196 SIVmac239-infected Indian rhesus macaques revealed that Mamu-B*08 was significantly overrepresented in elite controllers; 38% of elite controllers were Mamu-B*08 positive compared to 3% of progressors (P = 0.00001). Mamu-B*08 was also associated with a 7.34-fold decrease in chronic phase viremia (P = 0.002). Mamu-B*08-positive macaques may, therefore, provide a good model to understand the correlates of MHC class I allele-associated immune protection and viral containment in human elite controllers.

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Mhc class I haplotypes associated with survival time in simian immunodeficiency virus (SIV)-infected rhesus macaques

Genes and Immunity ◽

10.1038/sj.gene.6364448 ◽

2007 ◽

Vol 9 (1) ◽

pp. 69-80 ◽

Cited By ~ 45

Author(s):

U Sauermann ◽

R Siddiqui ◽

Y-S Suh ◽

M Platzer ◽

N Leuchte ◽

...

Keyword(s):

Survival Time ◽

Simian Immunodeficiency Virus ◽

Mhc Class I ◽

Rhesus Macaques ◽

Class I ◽

Immunodeficiency Virus

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MHC genotyping from rhesus macaque exome sequences

10.1101/625244 ◽

2019 ◽

Author(s):

John R. Caskey ◽

Roger W. Wiseman ◽

Julie A. Karl ◽

David A. Baker ◽

Taylor Lee ◽

...

Keyword(s):

Rhesus Macaque ◽

Mhc Class I ◽

Rhesus Macaques ◽

Class Ii ◽

Class I ◽

Target Enrichment ◽

High Confidence ◽

Protein Coding ◽

Histocompatibility Complex ◽

Mhc Genotyping

AbstractIndian rhesus macaque major histocompatibility complex (MHC) variation can influence the outcomes of transplantation and infectious disease studies. Frequently, rhesus macaques are MHC genotyped to identify variants that could account for unexpected results. Since the MHC is only one region in the genome where variation could impact experimental outcomes, strategies for simultaneously profiling variation in the macaque MHC and the remainder of the protein coding genome would be useful. Here we introduce macaque exome sequence (MES) genotyping, in which MHC class I and class II genotypes are determined with high confidence using target-enrichment probes that are enriched for MHC sequences. For a cohort of 27 Indian rhesus macaques, we describe two methods for obtaining MHC genotypes from MES data and demonstrate that the MHC class I and class II genotyping results obtained with these methods are 98.1% and 98.7% concordant, respectively, with expected MHC genotypes. In contrast, conventional MHC genotyping results obtained by deep sequencing of short multiplex PCR amplicons were only 92.6% concordant with expectations for this cohort.

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Spontaneous endometriosis in rhesus macaques: evidence for a genetic association with specific Mamu-A1 alleles

Primate Biology ◽

10.5194/pb-4-117-2017 ◽

2017 ◽

Vol 4 (1) ◽

pp. 117-125

Author(s):

Ivanela Kondova ◽

Gerco Braskamp ◽

Peter J. Heidt ◽

Wim Collignon ◽

Tom Haaksma ◽

...

Keyword(s):

Mhc Class I ◽

Macaca Mulatta ◽

Dna Sequences ◽

Clinical Symptoms ◽

Rhesus Macaques ◽

The United States ◽

Class I ◽

Research Centre ◽

Primate Research ◽

Increased Risk

Abstract. Endometriosis is a poorly understood common debilitating women's reproductive disorder resulting from proliferative and ectopic endometrial tissue associated with variable clinical symptoms including dysmenorrhea (painful menstrual periods), dyspareunia (pain on intercourse), female infertility, and an increased risk of malignant transformation. The rhesus macaque (Macaca mulatta) develops a spontaneous endometriosis that is very similar to that seen in women. We hypothesized that specific major histocompatibility complex (MHC) alleles may contribute to the pathogenesis of endometriosis. As part of a collaboration between the Biomedical Primate Research Centre (BPRC) in the Netherlands and the New England Primate Research Center (NEPRC) in the United States, we analyzed DNA sequences of MHC class I (Macaca mulatta, Mamu-A1) and class II (Mamu-DRB) alleles from rhesus macaques with endometriosis and compared the allele frequencies with those of age-matched healthy macaques. We demonstrate that two MHC class I alleles are overrepresented in diseased macaques compared to controls: Mamu-A1*001, 33.3 % in BPRC animals with endometriosis vs. 11.6 % in healthy macaques (p =  0.007), and Mamu-A1*007, 21.9 % NEPRC rhesus macaques vs. 6.7 %, (p =  0.003). We provide evidence that select MHC class I alleles are associated with endometriosis in rhesus macaques and suggest that the disease pathogenesis contribution of MHC class I warrants further research.

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