Optimization of the extracellular production of a bacterial phytase with Escherichia coli by using different fed-batch fermentation strategies

2003 ◽  
Vol 61 (5-6) ◽  
pp. 456-462 ◽  
Author(s):  
S. Kleist ◽  
G. Miksch ◽  
B. Hitzmann ◽  
M. Arndt ◽  
K. Friehs ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Batch Fermentation ◽  
Extracellular Production ◽  
Fed Batch ◽  
Fed Batch Fermentation

Co-Expression of Threonine Dehydratase and Acetolactate Synthase in Escherichia Coli for L-Isoleucine Production

2014 ◽  
Vol 989-994 ◽  
pp. 997-1002 ◽  
Author(s):  
Jian Wang ◽  
Jia Kai Sun ◽  
Qing Yang Xu
Keyword(s):  
Escherichia Coli ◽  
Corynebacterium Glutamicum ◽  
Carbon Flux ◽  
Batch Fermentation ◽  
Acetolactate Synthase ◽  
Fed Batch ◽  
Threonine Dehydratase ◽  
E Coli ◽  
Fed Batch Fermentation ◽  
Fermentation Period

Metabolic engineering ofCorynebacterium glutamicumhas sought to divert carbon into L-isoleucine. However, the fermentation period of this strain is long. TheC.glutamicumYILW strain (LeuL, AHVr, SGr, Leu-MEr) was previously derived by repeated compound mutagenesis which could accumulate 20.2 g/L L-isoleucine in a 5-L jar fermentor. Overexpression of the threonine dehydratase gene (ilvA) fromCorynebacterium glutamicumYILW and coexpression of threonine dehydratase and acetolactate synthase (ilvBN) from it were employed to divert carbon flux toward L-isoleucine. The strainE. coliTRFC with the expression ofilvA could accumulate L-isoleucine of 6.8 g/L without accumulation of any L-threonine by fed-batch fermentation in a 5-L jar fermentor. However, the production of L-isoleucine by the strainE.coliTRFC with the co-expression ofilvA andilvBN was decreased by 19.1%, and the production of L-valine was increased by 40% compared with that ofE. coliTRFC with the expression ofilvA.

Segregation to non-dividing cells in recombinant Escherichia coli fed-batch fermentation processes

2004 ◽  
Vol 26 (19) ◽  
pp. 1533-1539 ◽  
Author(s):  
Heléne Sundström ◽  
Fredrik Wållberg ◽  
Erika Ledung ◽  
Bo Norrman ◽  
Christopher J. Hewitt ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Batch Fermentation ◽  
Recombinant Escherichia Coli ◽  
Fed Batch ◽  
Fermentation Processes ◽  
Fed Batch Fermentation ◽  
Dividing Cells

Effects of betaine supplementation on l-threonine fed-batch fermentation by Escherichia coli

2018 ◽  
Vol 41 (10) ◽  
pp. 1509-1518 ◽  
Author(s):  
Yuewen Su ◽  
Qun-qun Guo ◽  
Sen Wang ◽  
Xin Zhang ◽  
Jian Wang
Keyword(s):  
Escherichia Coli ◽  
Batch Fermentation ◽  
Fed Batch ◽  
Fed Batch Fermentation ◽  
Betaine Supplementation

scholarly journals Optimization of the IPP-bypass mevalonate pathway and fed-batch fermentation for the production of isoprenol in Escherichia coli

2019 ◽  
Vol 56 ◽  
pp. 85-96 ◽  
Author(s):  
Aram Kang ◽  
Daniel Mendez-Perez ◽  
Ee-Been Goh ◽  
Edward E.K. Baidoo ◽  
Veronica T. Benites ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Batch Fermentation ◽  
Mevalonate Pathway ◽  
Fed Batch ◽  
Fed Batch Fermentation
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