Do free dance movements communicate how we feel? Investigating emotion recognition in dance

Current research into music and free dance movement explores differences in corporeal articulation of basic emotions. Accordingly, Van Dyck et al. (2014, https://doi.org/10.1371/journal.pone.0089773) report congruent emotion recognition in free dance movements recorded after happiness or sadness inductions in lay dancers. The current study replicates this previous study with an advanced methodological approach measuring ratings of happiness and sadness recognition separately within both happy and sad conditions. We then tested the differences between the recognition of happiness and sadness in free dance movements. Therefore, a dance movement pre-study was conducted in two different conditions where either happiness or sadness were induced within four lay dancers using guided imagery and music listening. Subsequent to this, dancers were video recorded while moving freely to a neutral piece of music. Those silenced video recordings were then presented to participants (N = 37) in an online experiment, who were instructed to rate the emotion they recognised. Based on the Effort-Shape Theory by Rudolf Laban, observers also rated kinematic features of velocity/acceleration, directness, impulsiveness and expansion. Participants rated higher levels of happiness for the happy-induction condition compared to sadness. However, participants rated higher levels of sadness in the sad condition compared to happiness for just one of the four dancers. This finding indicates that it is easier to recognise happiness in free dance movements than sadness. The results of the kinematic features supported previous research which rated higher intensities for the happy condition than the sad condition.

Establishment of callus induction system, histological evaluation and taxifolin production of Larch

Taxifolin in larch is an important natural active ingredient, which prevents and treats liver diseases and cardiovascular diseases, and has many health benefits and application values ​​such as anti-tumor, anti-virus, and anti-aging. However, the traditional extraction method is not conducive to the protection of the ecological environment, and also limits the industrial production of taxifolin from larch. Therefore, the purpose of this research is to establish a larch callus culture system and obtain taxifolin from callus. The shoots of the stem-tips of Larix olgensis were used as explants to be cultured. The light conditions, optimal medium and hormone ratio for callus induction were screened to evaluate the callus induction characteristics of larch and its influence on the accumulation of taxifolin. The results showed that: callus formation started on 28–51 days, and the best conditions for inducing callus were explored: 12 h/d light culture MS + 2,4-D 2 mg/L + 6-BA 1 mg/ L + KT 0.1 mg/L; the callus induction rate is 50%-80%; the highest condition for producing taxifolin 6-BA 1 mg/L + NAA 0.1 mg/L, reaching 0.414%. In conclusion, this study represents a suitable induction condition for larch callus culture and taxifolin accumulation.

Inhibition of Distinct Proline- or N-Acetylglucosamine-Induced Hyphal Formation Pathways by Proline Analogs in Candida albicans

Candida albicans undergoes a yeast-to-hyphal transition that has been recognized as a virulence property as well as a turning point leading to biofilm formation associated with candidiasis. It is known that yeast-to-hyphal transition is induced under complex environmental conditions including temperature (above 35°C), pH (greater than 6.5), CO2, N-acetylglucosamine (GlcNAc), amino acids, RPMI-1640 synthetic culture medium, and blood serum. To identify the hyphal induction factor in the RPMI-1640 medium, we examined each component of RPMI-1640 and established a simple hyphal induction condition, that is, incubation in L-proline solution at 37°C. Incubation in GlcNAc solution alone, which is not contained in RPMI-1640, without any other materials was also identified as another simple hyphal induction condition. To inhibit hyphal formation, proline and GlcNAc analogs were examined. Among the proline analogs used, L-azetidine-2-carboxylic acid (AZC) inhibited hyphal induction under both induction conditions, but L-4-thiazolidinecarboxylic acid (T4C) specifically inhibited proline-induced hyphal formation only, while α-N-methyl-L-proline (mPro) selectively inhibited GlcNAc-induced hyphal formation. Hyphal formation in fetal bovine serum was also inhibited by AZC or T4C together with mPro without affecting the proliferation of yeast form. These results indicate that these proline analogs are ideal inhibitors of yeast-to-hyphal transition in C. albicans.

Differential expression levels of CD151 enable enrichment of atrial cardiomyocytes derived from human induced-pluripotent stem cell

Introduction Human iPSCs-derived cardiomyocytes (hiPSCs-CMs) are heterogeneous populations that contain ventricular-like CMs (VCMs), atrial-like CMs (ACMs) and pacemaker cells. Isolation of pure populations of each hiPSCs-CM subtype corresponding to the target regions of the heart enables effective drug screening process and stable engraftment of hiPSCs-CMs (e.g. ventricular cardiomyocytes without impurities). Purpose Atrial and ventricular cardiomyocytes develop from distinct mesoderm populations, and many of different genes are expressed between two subtypes. Since our method of cardiomyocytes differentiation from hiPSCs mimics in vivo cardiomyocytes development, we hypothesized that two subtypes could be separated by differentially expressed genes in hiPSCs-CMs differentiation process. In this study, we focused cell surface genes which are useful for analysis by flow cytometry, and then identified cell surface marker that can distinguish atrial and ventricular cardiomyocytes from hiPSCs-CMs. Methods We performed an antibody-based screening using hiPSCs-CMs induced under atrial induction condition (AIC) and ventricular induction condition (VIC) by flow cytometry. To identify cell surface markers which enable discrimination of cardiac subtypes, we isolated the cell populations using the antibodies against the cell surface markers. Quantitative PCR was performed to analyze expression levels of subtype-specific genes in sorted cells. We confirmed subtype classification of cells using patch-clamp method. Results We identified CD151 as a novel candidate of atrial/ventricular selectable marker. The expression level of CD151 was low in most hiPSCs-CMs under AIC. In these cells, CD151-low cells highly expressed atrial genes compared to CD151-high cells. In contrast, the expression level of CD151 was high in most hiPSCs-CMs under VIC. In these cells, CD151-high cells highly expressed ventricular genes compared to CD151-low cells. Furthermore, we investigated the electrophysiological properties of CD151-high and -low cells using patch-clamp experiments. As expected, the cells showing atrial type action potential were enriched in AIC with low expression of CD151 (n=17). On the other hand, CD151-high cells (n=16) contained no atrial CMs, but mostly nodal like cells. In addition, CD151-low cells in AIC were affected with action potential duration by exposure of atrial specific channel blocker (4-aminopyridine) and activator (carbachol). In VIC, CD151-high cells (n=16) demonstrated ventricular type action potential property compared to CD151-low cells (n=21). Conclusion These results suggest that CD151 is a useful marker which can enrich ACMs from hiPSCs-CMs. Because these enriched ACMs are uniform population, it may be appropriate for atrial-selective drug screening. Additionally, this marker can reduce contaminated ACMs from hiPSCs-CMs cultured in VIC. Action potential of CD151-high/low CMs Funding Acknowledgement Type of funding source: Other. Main funding source(s): Takeda pharmaceutical company limited, Japan society for the promotion of science(JSPS) KAKENHI

Expression of synthetic Phytochelatin EC20 in E. Coli increases its biosorption capacity and cadmium resistance

In this study E. coli recombinant clones that express the EC20 synthetic phytochelatin intracellularly were constructed. The increasement of Cd2+ biosorption capacity, and, also, the increasement of resistance to this toxic metal were analyzed. A gene that encodes the synthetic phytochelatin EC20 was synthesized in vitro. The EC20 synthetic gene was amplified by PCR, inserted into the DNA cloning vectors pBluescript®KS+ and pGEM®-TEasy, and also into the expression vectors pTE [pET-28(a)® derivative] and pGEX-T4-2®. The obtained recombinant plasmids were employed for genetic transformation of E. coli: pBsKS-EC20 and pGEM-EC20, they were introduced into DH10B and DH5α strains, similarly to pTE-EC20 and pGEX-EC20 that were introduced into BL21 strain. The EC20 expression was confirmed by SDS-PAGE analysis. The recombinant clones’ resistances to Cd2+ were determined by MIC analyses. The MIC for Cd2+ of DH10B/pBKS-EC20 and DH10B/pGEM-EC20 were 2.5 mM (EC20 induced), and 0.312 mM (EC20 repressed); respectively, 16 and 2 times higher than the control DH10B/pBsKS (0.156 mM). The MIC for Cd2+ of BL21/pTE-EC20 was 10.0 mM (EC20 induced) and 2.5 mM (EC20 repressed), compared with the control BL21/pTE which was only 1.25 mM. Analysis of ICP-AES showed that BL21/pGEX-EC20, after growth on the condition of EC20 expression, absorbed 37.5% of Cd2+, and even when cultured into the non-induction condition of EC20 expression, it absorbed 11.5%. These results allow the conclusion that recombinant E. coli clones expressing the synthetic phytochelatin EC20 show increased capacity for Cd2+ biosorption and enhanced resistance to this toxic ion.

Mindfulness, Meditation, and Belief in Free Will/Determinism

Two experiments investigated the influence of mindfulness, meditation, and type of induction (free will, determinism, or neutral) on affect and beliefs in free will/determinism. In Experiment 1, it was found that high mindful participants reported experiencing more positive affect and less negative affect than low mindful participants. In the determinism induction condition in Experiment 2, high mindful participants scored higher on free will beliefs after they meditated, whereas low mindful participants scored lower on free will beliefs after they meditated. So it would seem that mindfulness can have differential effects for high and low mindful individuals. It was also found that high mindful participants (depending on the task and induction condition) sometimes had longer reaction times than low mindful participants. This could be an indication of high mindful participants having a higher decision boundary for some tasks or simply an indication that high mindful participants enjoyed some tasks more than others (i.e., processing fluency). In addition, the internal consistency of the questionnaires was compared to the internal consistency found for those questionnaires in previous research.

Exploring the role of humor in the management of existential anxiety

The current study addressed a topic that has both theoretical and applied importance, by examining the potential existential anxiety-buffering function of humor. Participants (N = 556; 55% female; M age = 37 years) completed a measure of trait coping humor before being randomly assigned to a mortality salience condition and a humor induction condition and then completing a measure of death-thought accessibility. ANOVA revealed main effects of trait coping humor, mortality salience and humor induction on death-thought accessibility in the expected directions. Coping humor interacted with mortality salience (F(1,439) = 14.47, p < 0.01) showing that low coping humor participants were more affected by the mortality salience manipulation. Coping humor also interacted with humor induction (F(1,439) = 8.94, p < 0.01) showing that low coping humor participants were more affected by the humor induction. Findings suggests that whilst trait coping humor appears to buffer the effects of mortality salience, those low in trait coping humor may benefit the most from interventions aimed at reducing existential anxiety via humor. The apparent beneficial effect of humor induction for individuals low in coping humor holds a promise of advancing our understanding of existential threat and, ultimately, providing a basis for interventions to improve mental health.

The Improvement of Flaw Detection by the Configuration of Uniform Eddy Current Probes

In this review, the principles to detect flaws with uniform eddy currents were presented based on the shape and orientation of the excitation coils and detection coils of the probe. Techniques are applied to detect flaws like cracks, especially on the weld zone surface, of test pieces of non-magnetic and ferromagnetic materials, and have unique features which are immune to the effects of lift-off. In the technique of interest, almost all the probe models developed are the type with tangential rectangular excitation coils. The induction condition and the flaw signal for each probe were discussed based on the shape and orientation of the excitation coils and detection coils of the probe. Finally, the challenge of increasing sensitivity to detect flaws with a uniform eddy current was also presented.

Wiping your conscience clean

The Macbeth effect is a proposed phenomenon, whereby feelings of immorality activate a desire to cleanse. Extensions of this theory suggest that cleansing alleviates immoral feelings, thus reducing the urge to engage in compensatory behaviors, such as volunteering. We examined the Macbeth effect and volunteerism in undergraduate students with high levels of obsessive-compulsive disorder contamination concerns ( n = 164). Participants underwent an immorality, anxiety, or neutral emotion-induction condition and subsequently cleansed their hands or performed a control task. For participants in the immorality condition, increased ratings of distress were associated with increased accessibility of cleansing words. Furthermore, individuals in the immorality condition who cleansed volunteered for significantly less time than those who did not cleanse. We discuss findings in relation to the literature on the Macbeth effect and mental contamination.