Detection of protein–protein interactions by a combination of a novel cytoplasmic membrane targeting system of recombinant proteins and fluorescence resonance energy transfer

2006 ◽  
Vol 70 (4) ◽  
pp. 451-457 ◽  
Author(s):  
Seiji Shibasaki ◽  
Kouichi Kuroda ◽  
Hoang Duc Nguyen ◽  
Tomoaki Mori ◽  
Wen Zou ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Fluorescence Resonance Energy Transfer ◽  
Recombinant Proteins ◽  
Protein Interactions ◽  
Cytoplasmic Membrane ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Membrane Targeting ◽  
Protein Protein Interactions ◽  
Fluorescence Resonance

scholarly journals Measuring cooperative Rev protein-protein interactions on Rev responsive RNA by fluorescence resonance energy transfer

RNA Biology ◽  
2011 ◽  
Vol 8 (2) ◽  
pp. 316-324 ◽  
Author(s):  
Thomas Vercruysse ◽  
Sonalika Pawar ◽  
Wim De Borggraeve ◽  
Els Pardon ◽  
George N. Pavlakis ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Fluorescence Resonance Energy Transfer ◽  
Protein Interactions ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Protein Protein Interactions ◽  
Fluorescence Resonance ◽  
Rev Protein

Fluorescence resonance energy transfer-based technologies in the study of protein–protein interactions at the cell surface

Methods ◽  
2012 ◽  
Vol 57 (4) ◽  
pp. 467-472 ◽  
Author(s):  
Víctor Fernández-Dueñas ◽  
Javier Llorente ◽  
Jorge Gandía ◽  
Dasiel O. Borroto-Escuela ◽  
Luigi F. Agnati ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Cell Surface ◽  
Fluorescence Resonance Energy Transfer ◽  
Protein Interactions ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Protein Protein Interactions ◽  
Fluorescence Resonance

Imaging protein–protein interactions in cell motility using fluorescence resonance energy transfer (FRET)

2004 ◽  
Vol 32 (3) ◽  
pp. 431-433 ◽  
Author(s):  
M. Parsons ◽  
B. Vojnovic ◽  
S. Ameer-Beg
Keyword(s):  
Energy Transfer ◽  
Fluorescence Resonance Energy Transfer ◽  
Conformational Changes ◽  
Protein Interactions ◽  
Intact Cell ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Live Cells ◽  
Protein Protein Interactions ◽  
Fluorescence Resonance

Protein–protein interactions and signal transduction pathways have traditionally been analysed using biochemical techniques or standard microscopy. Although invaluable in the delineation of protein hierarchy, these methods do not provide information on the true spatial and temporal nature of complex formation within the intact cell. Recent advances in microscopy have allowed the development of new methods to analyse protein–protein interactions at very high resolution in both fixed and live cells. The present paper provides a brief overview of using fluorescence resonance energy transfer to analyse directly molecular interactions and conformational changes in various proteins involved in the regulation of cell adhesion and motility.

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