fluorescence resonance
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2022 ◽  
Author(s):  
Sena Ghayyem ◽  
Farnoush Faridbod

Herein, we present a facile and sensitive fluorescence resonance energy transfer (FRET) aptasensor for detection of pathogenic bacteria, where Antibiotic-functionalized cerium oxide nanoparticles were served as an energy donor and...


2021 ◽  
Vol 12 (4) ◽  
pp. 361-367
Author(s):  
Priyadarshine Hewavitharanage ◽  
Launa Steele ◽  
Isaac Dickenson

Fluorescence resonance energy transfer (FRET) is a powerful tool used in a wide range of applications due to its high sensitivity and many other advantages. Co-encapsulation of a donor and an acceptor in nanoparticles is a useful strategy to bring the donor-acceptor pair in proximity for FRET. A highly efficient FRET system based on BODIPY-BODIPY (BODIPY:  boron-dipyrromethene) donor-acceptor pair in nanoparticles was synthesized. Nanoparticles were formed by co-encapsulating a green emitting BODIPY derivative (FRET donor, lmax = 501 nm) and a red emitting BODIPY derivative (FRET acceptor, lmax = 601 nm) in an amphiphilic polymer using the precipitation method. Fluorescence measurements of encapsulated BODIPY in water following 501 nm excitation caused a 3.6 fold enhancement of the acceptor BODIPY emission at 601 nm indicating efficient energy transfer between the green emitting donor BODIPY and the red emitting BODIPY acceptor with a 100 nm Stokes shift. The calculated FRET efficiency was 96.5%. Encapsulated BODIPY derivatives were highly stable under our experimental conditions.


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