Characterization of an exo-acting intracellular α-amylase from the hyperthermophilic bacterium Thermotoga neapolitana

2009 ◽  
Vol 86 (2) ◽  
pp. 555-566 ◽  
Author(s):  
Kyung-Min Park ◽  
So-Young Jun ◽  
Kyoung-Hwa Choi ◽  
Kwan-Hwa Park ◽  
Cheon-Seok Park ◽  
...  
Keyword(s):  
Thermotoga Neapolitana ◽  
Hyperthermophilic Bacterium

Molecular cloning and characterization of thermostable DNA ligase from Aquifex pyrophilus, a hyperthermophilic bacterium

Extremophiles ◽  
2001 ◽  
Vol 5 (3) ◽  
pp. 161-168 ◽  
Author(s):  
Jae-Hwan Lim ◽  
Juhyun Choi ◽  
Soo-Jin Han ◽  
Sung Kim ◽  
Hye-Zin Hwang ◽  
...  
Keyword(s):  
Molecular Cloning ◽  
Dna Ligase ◽  
Hyperthermophilic Bacterium ◽  
Aquifex Pyrophilus

Expression and Characterization of a Novel Nitrilase from Hyperthermophilic Bacterium Thermotoga maritima MSB8

2015 ◽  
Vol 25 (10) ◽  
pp. 1660-1669 ◽  
Author(s):  
Zhi Chen ◽  
Huayou Chen ◽  
Zhong Ni ◽  
Rui Tian ◽  
Tianxi Zhang ◽  
...  
Keyword(s):  
Thermotoga Maritima ◽  
Hyperthermophilic Bacterium

scholarly journals Capnophilic Lactic Fermentation from Thermotoga neapolitana: A Resourceful Pathway to Obtain Almost Enantiopure L-lactic Acid

Fermentation ◽  
2019 ◽  
Vol 5 (2) ◽  
pp. 34 ◽  
Author(s):  
Genoveffa Nuzzo ◽  
Simone Landi ◽  
Nunzia Esercizio ◽  
Emiliano Manzo ◽  
Angelo Fontana ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Enantiomeric Excess ◽  
Thermophilic Bacterium ◽  
Racemic Mixture ◽  
Thermotoga Neapolitana ◽  
High Concentration ◽  
Lactic Fermentation ◽  
Bacterial Fermentation ◽  
First Time

The industrial production of lactic acid (LA) is mainly based on bacterial fermentation. This process can result in enantiopure or racemic mixture according to the producing organism. Between the enantiomers, L-lactic acid shows superior market value. Recently, we reported a novel anaplerotic pathway called capnophilic lactic fermentation (CLF) that produces a high concentration of LA by fermentation of sugar in the anaerobic thermophilic bacterium Thermotoga neapolitana. The aim of this work was the identification of the enantiomeric characterization of the LA produced by T. neapolitana and identification of the lactate dehydrogenase in T. neapolitana (TnLDH) and related bacteria of the order Thermotogales. Chemical derivatization and GC/MS analysis were applied to define the stereochemistry of LA from T. neapolitana. A bioinformatics study on TnLDH was carried out for the characterization of the enzyme. Chemical analysis showed a 95.2% enantiomeric excess of L-LA produced by T. neapolitana. A phylogenetic approach clearly clustered the TnLDH together with the L-LDH from lactic acid bacteria. We report for the first time that T. neapolitana is able to produce almost enantiopure L-lactic acid. The result was confirmed by bioinformatics analysis on TnLDH, which is a member of the L-LDH sub-family.

Characterization of a extreme thermostable fructose-1,6-bisphosphate aldolase from hyperthermophilic bacterium Aquifex aeolicus

2009 ◽  
Vol 45 (4) ◽  
pp. 261-266 ◽  
Author(s):  
Im-Joung La ◽  
Da-Young Eum ◽  
Vinayakumar Gedi ◽  
Jinheung Kim ◽  
Byeongmoon Jeong ◽  
...  
Keyword(s):  
Aquifex Aeolicus ◽  
Hyperthermophilic Bacterium ◽  
Fructose 1,6 Bisphosphate

Biochemical characterization of a thermostable β-1,3-xylanase from the hyperthermophilic eubacterium, Thermotoga neapolitana strain DSM 4359

2012 ◽  
Vol 97 (15) ◽  
pp. 6749-6757 ◽  
Author(s):  
Fumiyoshi Okazaki ◽  
Nanami Nakashima ◽  
Chiaki Ogino ◽  
Yutaka Tamaru ◽  
Akihiko Kondo
Keyword(s):  
Biochemical Characterization ◽  
Thermotoga Neapolitana
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