Depolymerization of low-rank coal by extracellular fungal enzyme systems. III.In vitro depolymerization of coal humic acids by a crude preparation of manganese peroxidase from the white-rot fungus Nematoloma frowardii b19

1997 ◽  
Vol 47 (5) ◽  
pp. 566-571 ◽  
Author(s):  
M. Hofrichter ◽  
W. Fritsche
Keyword(s):  
Humic Acids ◽  
Manganese Peroxidase ◽  
White Rot ◽  
Low Rank ◽  
White Rot Fungus ◽  
Low Rank Coal ◽  
Enzyme Systems ◽  
Fungal Enzyme ◽  
Crude Preparation

Depolymerization of Straw Lignin by Manganese Peroxidase from Nematoloma frowardii is Accompanied by Release of Carbon Dioxide

Holzforschung ◽  
1999 ◽  
Vol 53 (2) ◽  
pp. 161-166 ◽  
Author(s):  
Martin Hofrichter ◽  
Katrin Scheibner ◽  
Friedemann Bublitz ◽  
Ivonne Schneegaß ◽  
Dirk Ziegenhagen ◽  
...  
Keyword(s):  
Carbon Dioxide ◽  
Manganese Peroxidase ◽  
Gel Permeation Chromatography ◽  
Water Soluble ◽  
White Rot ◽  
White Rot Fungus ◽  
Lignin Fraction ◽  
Molecular Masses ◽  
Total Lignin ◽  
Soluble Part

SummaryManganese peroxidase preparations (MnP) from the white-rot fungusNematoloma frowardiiwere able to release14CO2directly from14C-labeled milled wheat straw (MWS; total lignin fraction) and milled straw lignin (MSL; dioxane soluble part of MWS). Apart from the formation of14CO2(4–10 %) the treatment of insoluble MWS and MSL with MnP resulted in the formation of water-soluble14C-lignin fragments (lignin solubilization, 14–25%). Analyses with gel permeation chromatography (GPC) demonstrated the formation of lignin fragments with predominant molecular masses around 1 kDa. The extent of MWS mineralization and solubilization was enhanced in the presence of reduced glutathione (GSH) acting as thiol mediator, whereas MSL mineralization was not stimulated by GSH. The principle of direct extracellular mineralization of lignin catalyzed by the MnP system may make a significant contribution to the formation of carbon dioxide in lignincellulose containing habitats.

Degradation of lignite (low-rank coal) by ligninolytic basidiomycetes and their manganese peroxidase system

1999 ◽  
Vol 52 (1) ◽  
pp. 78-84 ◽  
Author(s):  
M. Hofrichter ◽  
D. Ziegenhagen ◽  
S. Sorge ◽  
R. Ullrich ◽  
F. Bublitz ◽  
...  
Keyword(s):  
Manganese Peroxidase ◽  
Low Rank ◽  
Low Rank Coal
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