Efficient production of medium-chain-length poly(3-hydroxyalkanoates) from octane by Pseudomonas oleovorans : economic considerations

1997 ◽  
Vol 48 (5) ◽  
pp. 588-596 ◽  
Author(s):  
W. Hazenberg ◽  
B. Witholt
Keyword(s):  
Chain Length ◽  
Efficient Production ◽  
Pseudomonas Oleovorans ◽  
Medium Chain ◽  
Medium Chain Length ◽  
Economic Considerations

Characterization of bionanocomposites based on medium chain length polyhydroxyalkanoates synthesized by Pseudomonas oleovorans

2010 ◽  
Vol 29 (8) ◽  
pp. 966-971 ◽  
Author(s):  
Sylvain Chardron ◽  
Stéphane Bruzaud ◽  
Brigitte Lignot ◽  
Anne Elain ◽  
Olivier Sire
Keyword(s):  
Chain Length ◽  
Pseudomonas Oleovorans ◽  
Medium Chain ◽  
Medium Chain Length

scholarly journals Production of medium-chain-length polyhydroxyalkanoate by Pseudomonas oleovorans grown in sugary cassava extract supplemented with andiroba oil

2014 ◽  
Vol 34 (4) ◽  
pp. 738-745 ◽  
Author(s):  
Diego Aires da Silva ◽  
Regina Vasconcellos Antonio ◽  
José Márcio Rossi ◽  
Rosinelson da Silva Pena
Keyword(s):  
Chain Length ◽  
Pseudomonas Oleovorans ◽  
Medium Chain ◽  
Medium Chain Length

scholarly journals Transcriptome Changes in Pseudomonas putida KT2440 during Medium-Chain-Length Polyhydroxyalkanoate Synthesis Induced by Nitrogen Limitation

2020 ◽  
Vol 22 (1) ◽  
pp. 152
Author(s):  
Dorota Dabrowska ◽  
Justyna Mozejko-Ciesielska ◽  
Tomasz Pokój ◽  
Slawomir Ciesielski
Keyword(s):  
Chain Length ◽  
Nitrogen Limitation ◽  
Stringent Response ◽  
Wild Type ◽  
Pseudomonas Putida Kt2440 ◽  
Medium Chain ◽  
Environmental Stimuli ◽  
Medium Chain Length ◽  
In The Wild

Pseudomonas putida’s versatility and metabolic flexibility make it an ideal biotechnological platform for producing valuable chemicals, such as medium-chain-length polyhydroxyalkanoates (mcl-PHAs), which are considered the next generation bioplastics. This bacterium responds to environmental stimuli by rearranging its metabolism to improve its fitness and increase its chances of survival in harsh environments. Mcl-PHAs play an important role in central metabolism, serving as a reservoir of carbon and energy. Due to the complexity of mcl-PHAs’ metabolism, the manner in which P. putida changes its transcriptome to favor mcl-PHA synthesis in response to environmental stimuli remains unclear. Therefore, our objective was to investigate how the P. putida KT2440 wild type and mutants adjust their transcriptomes to synthesize mcl-PHAs in response to nitrogen limitation when supplied with sodium gluconate as an external carbon source. We found that, under nitrogen limitation, mcl-PHA accumulation is significantly lower in the mutant deficient in the stringent response than in the wild type or the rpoN mutant. Transcriptome analysis revealed that, under N-limiting conditions, 24 genes were downregulated and 21 were upregulated that were common to all three strains. Additionally, potential regulators of these genes were identified: the global anaerobic regulator (Anr, consisting of FnrA, Fnrb, and FnrC), NorR, NasT, the sigma54-dependent transcriptional regulator, and the dual component NtrB/NtrC regulator all appear to play important roles in transcriptome rearrangement under N-limiting conditions. The role of these regulators in mcl-PHA synthesis is discussed.

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