Donor age and long-term culture affect differentiation and proliferation of human bone marrow mesenchymal stem cells

2012 ◽  
Vol 91 (8) ◽  
pp. 1175-1186 ◽  
Author(s):  
Merve Zaim ◽  
Serap Karaman ◽  
Guven Cetin ◽  
Sevim Isik
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Donor Age ◽  
Long Term Culture ◽  
Marrow Mesenchymal Stem Cells ◽  
Differentiation And Proliferation

scholarly journals The Effect of Culture on Human Bone Marrow Mesenchymal Stem Cells: Focus on DNA Methylation Profiles

2016 ◽  
Vol 2016 ◽  
pp. 1-12 ◽  
Author(s):  
Angela Bentivegna ◽  
Gaia Roversi ◽  
Gabriele Riva ◽  
Laura Paoletta ◽  
Serena Redaelli ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Differentiation Potential ◽  
Marrow Mesenchymal Stem Cells

Human bone marrow mesenchymal stem cells (hBM-MSCs) are the best characterized multipotent adult stem cells. Their self-renewal capacity, multilineage differentiation potential, and immunomodulatory properties have indicated that they can be used in many clinical therapies. In a previous work we studied the DNA methylation levels of hBM-MSC genomic DNA in order to delineate a kind of methylation signature specific for early and late passages of culture. In the present work we focused on the modification of the methylation profiles of the X chromosome and imprinted loci, as sites expected to be more stable than whole genome. We propose a model where cultured hBM-MSCs undergo random modifications at the methylation level of most CGIs, nevertheless reflecting the original methylation status. We also pointed out global genome-wide demethylation connected to the long-term culture and senescence. Modification at CGIs promoters of specific genes could be related to the decrease in adipogenic differentiation potential. In conclusion, we showed important changes in CGIs methylation due to long-termin vitroculture that may affect the differentiation potential of hBM-MSCs. Therefore it is necessary to optimize the experimental conditions forin vitroexpansion in order to minimize these epigenetic changes and to standardize safer procedures.

scholarly journals Genome-scale DNA methylation pattern profiling of human bone marrow mesenchymal stem cells in long-term culture

2012 ◽  
Vol 44 (8) ◽  
pp. 503 ◽  
Author(s):  
Mi Ran Choi ◽  
Yong-Ho In ◽  
Jungsun Park ◽  
Taesung Park ◽  
Kyoung Hwa Jung ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Methylation Pattern ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Marrow Mesenchymal Stem Cells ◽  
Genome Scale

scholarly journals Structural investigation of donor age effect on human bone marrow mesenchymal stem cells: FTIR spectroscopy and imaging

AGE ◽  
2014 ◽  
Vol 36 (4) ◽  
Author(s):  
Ceren Aksoy ◽  
Fatima Aerts Kaya ◽  
Bülent Barış Kuşkonmaz ◽  
Duygu Uçkan ◽  
Feride Severcan
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Ftir Spectroscopy ◽  
Structural Investigation ◽  
Age Effect ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Donor Age ◽  
Marrow Mesenchymal Stem Cells

Candidate kinases for adipogenesis and osteoblastogenesis from human bone marrow mesenchymal stem cells

2021 ◽  
Author(s):  
Xia Yi ◽  
Ping Wu ◽  
Jianyun Liu ◽  
Shan He ◽  
Ying Gong ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Transcription Factors ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Cytoplasmic Proteins ◽  
Marrow Mesenchymal Stem Cells

Adipogenesis and osteoblastogenesis (adipo-osteoblastogenesis) are closely related processes involving with the phosphorylation of numerous cytoplasmic proteins and key transcription factors.

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