Delineating Toxicity Mechanisms Associated with MRI Contrast Enhancement through a Multidimensional Toxicogenomic Profiling of Gadolinium

Although gadolinium is widely used for magnetic resonance imaging in clinical settings, many concerns regarding its toxicity and bioaccumulation after gadolinium-based contrast agent (GBCA) administration have been raised and published...

Laser capture microdissection-capillary zone electrophoresis-tandem mass spectrometry (LCM-CZE-MS/MS) for spatially resolved top-down proteomics: a pilot study of zebrafish brain

Mass spectrometry (MS)-based spatially resolved top-down proteomics (TDP) of tissues is crucial for understanding the roles played by microenvironmental heterogeneity in the biological functions of organs and for discovering new...

Integrated Proteomic and Metabolomic Analyses of the Mitochondrial Neurodegenerative Disease MELAS

MELAS (mitochondrial encephalomyopathy, lactic acidosis, stroke-like episodes) is a progressive neurodegenerative disease caused by pathogenic mitochondrial DNA variants. The pathogenic mechanism of MELAS remains enigmatic due to the exceptional clinical...

Critical assessment of genome-scale metabolic models of Arabidopsis thaliana

Genome-scale metabolic models (GSMMs) have enabled researchers to perform systems-level studies of living organisms. As a constraint-based technique, flux balance analysis (FBA) aids computation of reaction fluxes and prediction of...

Multi-omic profiling of histone variant H3.3 lysine 27 methylation reveals a distinct role from canonical H3 in stem cell differentiation

Histone variants, such as histone H3.3, replace canonical histones within the nucleosome to alter chromatin accessibility and gene expression. Although the biological roles of selected histone post-translational modifications (PTMs) have...

Proteomic and metabolomic profiling of acute and chronic stress events associated with military exercises

Saliva collected from military personnel during training yields potential biomarkers that could be utilized to differentiate types of stress, specifically chronic versus acute.

Multi-omic characterisation of Streptomyces hygroscopicus NRRL 30439: detailed assessment of its secondary metabolic potential

The emergence of multidrug-resistant pathogenic bacteria creates a demand for novel antibiotics with distinct mechanisms of action. Advances in next-generation genome sequencing promised a paradigm shift in the quest to...

An integrative chemometric approach and correlative metabolite networking of LC-MS and 1H NMR based urine metabolomics for radiation signatures

A comprehensive overview of combining data from LC-MS and NMR using multiblock-OPLSDA analysis, correlation networking and pathway enrichment. This provided novel avenues for understanding biological perturbations post radiation injury.

Investigation of cattle plasma proteome in response to pain and inflammation using next generation proteomics technique, SWATH-MS

SWATH-MS provides comprehensive protein profile of cattle plasma in response to tissue injury induced pain and inflammation.

1H-NMR based metabolic study of MMTV-PyMT mice along with pathological progress to screen biomarkers for early diagnosis of breast cancer

1H NMR based metabonomic approach was applied to monitor the alterations of serum metabolic profiles in MMTV-PyMT transgenic mice to detect the dynamic changes related to the pathological process and...