Organization of chloroplast ribosomal RNA genes and in vitro self-splicing activity of the large subunit rRNA intron from the green alga Chlorella vulgaris C-27

1997 ◽  
Vol 31 (6) ◽  
pp. 503-510 ◽  
Author(s):  
M. Kapoor ◽  
T. Nagai ◽  
T. Wakasugi ◽  
K. Yoshinaga ◽  
M. Sugiura
Keyword(s):  
Green Alga ◽  
Ribosomal Rna ◽  
Chlorella Vulgaris ◽  
Large Subunit ◽  
Ribosomal Rna Genes ◽  
Green Alga Chlorella ◽  
Large Subunit Rrna ◽  
Self Splicing ◽  
Rna Genes

Eukaryotic diversity and phylogeny using small- and large-subunit ribosomal RNA genes from environmental samples

2009 ◽  
Vol 11 (12) ◽  
pp. 3179-3188 ◽  
Author(s):  
William Marande ◽  
Purificación López-García ◽  
David Moreira
Keyword(s):  
Ribosomal Rna ◽  
Environmental Samples ◽  
Large Subunit ◽  
Ribosomal Rna Genes ◽  
Eukaryotic Diversity ◽  
Rna Genes ◽  
Large Subunit Ribosomal Rna

In vitro Transcription of Escherichia coli Ribosomal RNA Genes

Nature ◽  
1972 ◽  
Vol 235 (5337) ◽  
pp. 329-333 ◽  
Author(s):  
WILLIAM A. HASELTINE
Keyword(s):  
Escherichia Coli ◽  
Ribosomal Rna ◽  
In Vitro Transcription ◽  
Ribosomal Rna Genes ◽  
Rna Genes

In vitro transcription of the ribosomal RNA genes of E. coli DNA

1977 ◽  
Vol 151 (3) ◽  
pp. 305-312 ◽  
Author(s):  
János Sümegi ◽  
Andor Udvardy ◽  
Pál Venetianer
Keyword(s):  
Ribosomal Rna ◽  
In Vitro Transcription ◽  
Ribosomal Rna Genes ◽  
E Coli ◽  
Rna Genes

scholarly journals Transcription initiation of Xenopus 5S ribosomal RNA genes in vitro

1979 ◽  
Vol 7 (4) ◽  
pp. 947-958 ◽  
Author(s):  
Laurence J. Korn ◽  
Edward H. Birkenmeier ◽  
Donald D. Brown
Keyword(s):  
Ribosomal Rna ◽  
Transcription Initiation ◽  
Ribosomal Rna Genes ◽  
5S Ribosomal Rna ◽  
Rna Genes

Phylogeny of mitosporic entomopathogenic fungi: Is the genus Paecilomyces polyphyletic?

2001 ◽  
Vol 47 (9) ◽  
pp. 813-819 ◽  
Author(s):  
Miroslav Oborník ◽  
Milan Jirku ◽  
David Dolezel
Keyword(s):  
Ribosomal Rna ◽  
Entomopathogenic Fungi ◽  
Large Subunit ◽  
Sister Group ◽  
Small Subunit ◽  
Verticillium Lecanii ◽  
Rrna Gene ◽  
Small Subunit Rrna ◽  
Large Subunit Rrna ◽  
Rna Genes

We analyzed sequences of the divergent domain at the 5' end of the large subunit rRNA gene from the mitosporic entomopathogenic fungi Paecilomyces sp., Paecilomyces fumosoroseus, Paecilomyces farinosus, Paecilomyces lilacinus, Verticillium lecanii, Verticillium psalliotae, Beauveria bassiana, Aschersonia sp., Aschersonia placenta, ascomycetous Cordyceps sp., and Cordyceps militaris. Phylogenetic analysis showed P. fumosorseus as the best characterized out of the analyzed species with the B. bassiana clade as its sister group. Two of the P. farinosus isolates were invariably placed within the Verticillium cluster, which also contained C. militaris. The only analyzed P. lilacinus isolate appeared on the root of the hyphomycetous fungi and was characterized as the most distinct from all the hyphomycetous fungi tested. Polyphyly of the genus Paecilomyces was well supported by the Kishino-Hasegawa test. In all trees based on the small subunit rRNA gene sequences obtained from the GenBank(tm), V. lecanii, V. psalliotae, P. fumosoroseus, P. tenuipes and B. bassiana form, together with that of C. militaris, the best supported cluster in the tree. The rest of Cordyceps spp. constitute a distinct clade. Phylogenetic relationships derived from both tested DNA regions show polyphyly of the genus Paecilomyces and close relationships among entomopathogenic species of the genera Verticillium, Paecilomyces, and Beauveria.Key words: Paecilomyces, Verticillium, Beauveria, Aschersonia, entomopathogenic fungi, molecular phylogeny, ribosomal RNA genes.

Study of the function of Escherichia coli ribosomal RNA through site-directed mutagenesis

1990 ◽  
Vol 68 (1) ◽  
pp. 169-179 ◽  
Author(s):  
Daniel Leclerc ◽  
Léa Brakier-Gingras
Keyword(s):  
Escherichia Coli ◽  
Ribosomal Rna ◽  
Ribosomal Proteins ◽  
Large Body ◽  
Ribosomal Rna Genes ◽  
16S Ribosomal Rna ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
Rna Genes

Various approaches have been developed to study how mutations in Escherichia coli ribosomal RNA affect the function of the ribosome. Most of them are in vivo approaches, where mutations are introduced in a specialized plasmid harboring the ribosomal RNA genes. The mutated plasmids are then expressed in an appropriate host, where they can confer resistance to antibiotics whose target is the ribosome. Conditions can be used where the host ribosomal RNA genes or the host ribosomes are selectively inactivated, and the effect of the mutations on ribosome assembly and function can be studied. Another approach, which has been developed mainly with 16S ribosomal RNA, can be used entirely in vitro. In this approach, a plasmid has been constructed which contains the 16S ribosomal RNA gene under control of a T7 promoter. Mutations can be introduced in the 16S ribosomal RNA sequence and the mutated 16S ribosomal RNAs are produced by in vitro transcription. It is then possible to investigate how the mutations affect the assembly of the 16S ribosomal RNA into 30S subunits and the activity of the reconstituted 30S subunits in cell-free protein synthesis assays. Although these approaches are recent, they have already provided a large body of interesting information, relating specific RNA sequences to interactions with ribosomal proteins, to ribosome function, and to its response to antibiotics.Key words: ribosomal RNA, ribosome, site-directed mutagenesis, antibiotic resistance.

The chloroplast chlL gene of the green alga Chlorella vulgaris C-27 contains a self-splicing group I intron

1996 ◽  
Vol 250 (6) ◽  
pp. 655-664 ◽  
Author(s):  
Meenu Kapoor ◽  
T. Wakasugi ◽  
Koichi Yoshinaga ◽  
M. Sugiura
Keyword(s):  
Green Alga ◽  
Chlorella Vulgaris ◽  
Group I Intron ◽  
Group I ◽  
Green Alga Chlorella ◽  
Self Splicing

scholarly journals Effect of lucanthone (miracil D) on transcription of ribosomal RNA genes from Tetrahymena in vivo and in vitro

1979 ◽  
Vol 6 (7) ◽  
pp. 2391-2402 ◽  
Author(s):  
Ole Westergaard ◽  
Elmar Gocke ◽  
Ole F. Nielsen ◽  
Johan C. Leer
Keyword(s):  
Ribosomal Rna ◽  
Ribosomal Rna Genes ◽  
Rna Genes

The large subunit ribosomal RNA genes of Entrophospora infrequens comprise sequences related to two different glomalean families

2001 ◽  
Vol 152 (1) ◽  
pp. 159-167 ◽  
Author(s):  
A. Rodriguez ◽  
T. Dougall ◽  
J. C. Dodd ◽  
J. P. Clapp
Keyword(s):  
Ribosomal Rna ◽  
Large Subunit ◽  
Ribosomal Rna Genes ◽  
Rna Genes ◽  
Large Subunit Ribosomal Rna
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