An Agrobacterium tumefaciens transformation protocol effective on a variety of cottonwood hybrids (genus Populus )

2000 ◽  
Vol 19 (3) ◽  
pp. 315-320 ◽  
Author(s):  
K.-H. Han ◽  
R. Meilan ◽  
C. Ma ◽  
S. H. Strauss
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Transformation Protocol ◽  
Agrobacterium Tumefaciens Transformation

scholarly journals Agrobacterium tumefaciens Transformation of the Radiation Hypersensitive Arabidopsis thaliana Mutants uvh1 and rad5

1998 ◽  
Vol 11 (11) ◽  
pp. 1136-1141 ◽  
Author(s):  
Jaesung Nam ◽  
Kirankumar S. Mysore ◽  
Stanton B. Gelvin
Keyword(s):  
Arabidopsis Thaliana ◽  
Agrobacterium Tumefaciens ◽  
Transformation Process ◽  
Stable Transformation ◽  
Wild Type ◽  
Dna Integration ◽  
Agrobacterium Tumefaciens Transformation ◽  
Inoculation Assay

The Arabidopsis thaliana mutants uvh1 and rad5, originally identified as radiation hypersensitive, were reported to be deficient in T-DNA integration based on the relative efficiencies of stable transformation and T-DNA transfer. We reassessed these mutants for susceptibility to transformation by Agrobacterium tumefaciens. The mutant rad5 showed a significant reduction in the efficiency of transient as well as stable transformation, compared with its wild-type progenitor. These data indicate that rad5 is blocked at a step in the transformation process prior to T-DNA integration. We additionally found, using both an in vitro root inoculation and an in vivo flower bolt inoculation assay, that the mutant uvh1 is as susceptible to A. tumefaciens-mediated transformation as is its wild-type progenitor, C10.

scholarly journals Identification of genes associated with asexual reproduction in Phyllosticta citricarpa mutants obtained through Agrobacterium tumefaciens transformation

2016 ◽  
Vol 192 ◽  
pp. 142-147 ◽  
Author(s):  
Eduardo Henrique Goulin ◽  
Daiani Cristina Savi ◽  
Desirrê Alexia Lourenço Petters ◽  
Vanessa Kava ◽  
Lygia Galli-Terasawa ◽  
...  
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Asexual Reproduction ◽  
Phyllosticta Citricarpa ◽  
Agrobacterium Tumefaciens Transformation

scholarly journals Optimization of Agrobacterium tumefaciens mediated genetic transformation protocol for aromatic ric

1970 ◽  
Vol 7 (2) ◽  
pp. 235-240
Author(s):  
MR Hossain ◽  
L Hassan ◽  
AK Patwary ◽  
MI Ferdous
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Genetic Transformation ◽  
Aromatic Rice ◽  
Rice Varieties ◽  
Transformation Protocol ◽  
Mature Embryos ◽  
Gus Reporter ◽  
Established Plant ◽  
Hygromycin Resistance Gene ◽  
Almost All

A rapid, efficient and reproducible genetic transformation protocol was optimized for four aromatic rice varieties by using the established plant regeneration protocol. Mature embryos were inoculated with Agrobacterium tumefaciens strain EHA105 carrying a binary vector pIG121-Hm with GUS (reporter gene) and hpt (hygromycin resistance) gene and the transformation experiment was performed by optimizing two important parameters viz. infection times and co-cultivation periods. The highest response to GUS assay was showed by Kalizira (70% GUS positive) followed by Pusa Basmati 1 (66.67%) when the explants were inoculated for 25 minutes and co-cultivated for three days. Twenty five minutes infection time (9.44%) and three day co- cultivation period (8.06%) were found effective for percentage of transgenic shoot regeneration. The highest percentage of putative transgenic shoots was regenerated by variety Kalizira (33.33%) followed by Pusa Basmati 1 (20.0%) and Radhunipagol (13.33%). Among the varieties, Kalizira produced the highest percentage (60%) of rooted shoots. Kalizira also showed the highest survival rate in growth chamber (75%) and in field condition (60%). The performance of Tulsimala was poor for almost all the cases. Keywords: Agrobacterium; GUS; hpt gene; Transformation and Aromatic rice DOI: 10.3329/jbau.v7i2.4724 J. Bangladesh Agril. Univ. 7(2): 235-240, 2009

Agrobacterium tumefaciens-mediated transformation protocol of Jatropha curcas L. using leaf and hypocotyl segments

2011 ◽  
Vol 21 (1) ◽  
pp. 128-133 ◽  
Author(s):  
Pratibha Misra ◽  
Dibya Dolridge Toppo ◽  
Manoj Kumar Mishra ◽  
Syed Saema ◽  
Gaurav Singh
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Jatropha Curcas ◽  
Transformation Protocol ◽  
Jatropha Curcas L ◽  
Hypocotyl Segments

scholarly journals Agrobacterium tumefaciens mediated transformation of the aquatic carnivorous plant Utricularia gibba

2020 ◽  
Author(s):  
Araceli Oropeza-Aburto ◽  
Sergio Alan Cervantes-Perez ◽  
Victor A Albert ◽  
Luis Rafael Herrera-Estrella
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Genetic Transformation ◽  
In Vitro Selection ◽  
Carnivorous Plant ◽  
Carnivorous Plants ◽  
35S Promoter ◽  
Transformation Protocol ◽  
Utricularia Gibba

Abstract Background The genus Utricularia belongs to Lentibulariaceae, the largest family of carnivorous plants, which includes terrestrial, epiphytic and aquatic species. The development of specialized structures that evolved for carnivory is a feature of this genus that has been of great interest to biologists since Darwin‘s early studies. Utricularia gibba is itself an aquatic plant with sophisticated bladder traps having one of the most complex suction mechanisms for trapping prey. However, the molecular characterization of the mechanisms that regulate trap development and the biophysical processes involved in prey trapping are still largely unknown due to the lack of a simple and reproducible gene transfer system. Results Here, we report the establishment of a simple, fast and reproducible protocol for genetic transformation of U. gibba based on the T-DNA of Agrobacterium tumefaciens . An in vitro selection system using Phosphinotricin as a selective agent was established for U. gibba . Plant transformation was confirmed by histochemical GUS assays and PCR and qRT-PCR analyses. We report on the expression pattern of the 35S promoter and of the promoter of a trap-specific ribonuclease gene in transgenic U. gibba plants. Conclusions The genetic transformation protocol reported here is an effective method for studying developmental biology and functional genomics of this genus of carnivorous plants and advances the utility of U. gibba as a model system to study developmental processes involved in trap formation.

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