In vitro induction of multiple shoots and plant regeneration in cotton (Gossypium hirsutum L.)

In plant tissue culture studies, obtaining new plantlets from different parts of plant is a very important feature with direct or indirect ways of regeneration. The plant show different regeneration capacities from species to species. In this present study, in-vitro direct shoots development from embryonic axis of cotton (Gossypium hirsutum L. cv. Lashata) were comparatively studied. Embryonic axis, embryonic hypocotyl and plumule obtained from five days old in-vitro grown seeds. All explants were cultured on MS media supplemented with 1- 4 mg/l Benzyl amino purine (BAP) + 0.1 mg/l Naphthalene acetic acid (NAA) and 3 mg/l activated charcoal. Cultured explants were kept at growth chamber with photoperiod for 16 hours light and 8 hours dark, at 25oC. Reproducible in-vitro plant regeneration was obtained from embryonic axis when cultured onto MS medium supplemented 1 mg/l BAP + 0.1 mg/l NAA and 3 g/l Activated charcoal. Shoot induction was to be noted 53.3%, 36.7% and 12.3 in embryonic axis, plumule and embryonic hypocotyl respectively. All these shoots were capable of rooting on MS medium supplemented with 0.1 mg/l NAA, and establishing in soil 3-4 weeks.

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High frequency induction of multiple shoots and plant regeneration from zygotic embryo axis explants of cotton cultivar L-604 (Gossypium hirsutum L.).

International Journal of Bioassays ◽

10.21746/ijbio.2016.09.002 ◽

2016 ◽

Vol 5 (09) ◽

pp. 4791

Author(s):

Vijaya Naresh Juturu ◽

Gopala Krishna Mekala ◽

Akila Chandra Sekhar ◽

Puli Chandra Obul Reddy ◽

Mallikarjuna Garladinne*

Keyword(s):

Gossypium Hirsutum ◽

Zygotic Embryo ◽

Multiple Shoots ◽

Shoot Elongation ◽

Naphthalene Acetic Acid ◽

Embryo Axis ◽

Gossypium Hirsutum L ◽

In Vitro Shoots ◽

Elite Cultivar

An efficient, robust regeneration protocol has been developed using zygotic embryo axis explants of an elite cultivar L-604 (Gossypium hirsutum L.). Explants regenerated on MSB5 medium supplemented with 0.5 mg-l Thidiazuron, 0.1 mg-l Naphthalene acetic acid, 5.0 mg-l Silver nitrate and 1.0 g-l activated charcoal, was identified as the best combination for producing the maximum number (12.67) of multiple shoots per explant. In addition, the highest shoot elongation (5.30 cm) and maximum regeneration frequency (89.0%) was also recorded in the same hormonal combinations. In vitro shoots were successfully rooted on ½ MSB5 medium containing 1.5 mg-l IBA. Well rooted plants were subsequently transferred to polyhouse and greenhouse conditions for primary and secondary hardenings respectively and about 95% of the plants were established successfully. The conditions optimized in this study for inducing multiple shoots could be best suitable for development of transgenic L-604 variety for agronomically important traits.

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In vitro induction of multiple shoots and plant regeneration inVigna

In Vitro Cellular & Developmental Biology - Plant ◽

10.1007/bf02822733 ◽

1998 ◽

Vol 34 (4) ◽

pp. 276-280 ◽

Cited By ~ 20

Author(s):

Jayanti Sen ◽

Sipra Guha-Mukherjee

Keyword(s):

Plant Regeneration ◽

Multiple Shoots ◽

In Vitro Induction

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Induction of multiple shoots and plant regeneration from ‘accessory buds’ of nodal segments from field-grown mature cotton plants (Gossypium hirsutum L.)

In Vitro Cellular & Developmental Biology - Plant ◽

10.1007/s11627-001-0138-3 ◽

2001 ◽

Vol 37 (6) ◽

pp. 830-834 ◽

Cited By ~ 3

Author(s):

Sulekha Hazra ◽

Dinesh C. Agrawal ◽

Anjan K. Banerjee ◽

Kaza V. Krishnamurthy ◽

Satish M. Nalawade

Keyword(s):

Plant Regeneration ◽

Gossypium Hirsutum ◽

Multiple Shoots ◽

Nodal Segments ◽

Gossypium Hirsutum L ◽

Cotton Plants

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In vitro Propagation of Polygonum hydropiper L. from Shoot Tips

Plant Tissue Culture and Biotechnology ◽

10.3329/ptcb.v20i1.5970 ◽

2010 ◽

Vol 20 (1) ◽

pp. 73-79 ◽

Cited By ~ 2

Author(s):

M. F. Hasan ◽

B. Sikdar

Keyword(s):

Plant Regeneration ◽

In Vitro Propagation ◽

Multiple Shoots ◽

Multiple Shoot ◽

Shoot Tips ◽

Root Induction ◽

Shoot Induction ◽

Multiple Shoot Induction ◽

Polygonum Hydropiper

An efficient protocol for plant regeneration through multiple shoots induction from shoot tips of Polygonum hydropiper (L.) was established. The highest percentage (96.6) of multiple shoot induction and number of shoots (9.0) per culture were found on MS supplemented with 2.0 mg/l Kn. The induced shoots were excised and inoculated on to MS contains different concentrations of IBA or NAA for rooting. The highest percentage (90.0) of root induction and the highest number of roots per shoot (12.0) was found on MS having 1.0 mg/l IBA. Well rooted plantlets were acclimated properly and transplanted in the soil under natural condition, where cent per cent plantlets survived and grew successfully. Key words: Polygonum hydropiper, Shoot tips, In vitro propagation D.O.I. 10.3329/ptcb.v20i1.5970 Plant Tissue Cult. & Biotech. 20(1): 73-79, 2010 (June)

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