A genetic approach to in vitro regeneration of non-regenerating cotton (Gossypium hirsutum L.) cultivars

1998 ◽  
Vol 18 (1-2) ◽  
pp. 59-63 ◽  
Author(s):  
S. Kumar ◽  
P. Sharma ◽  
D. Pental
Keyword(s):  
Gossypium Hirsutum ◽  
In Vitro Regeneration ◽  
Genetic Approach ◽  
Gossypium Hirsutum L

In Vitro Regeneration of Four Commercial Cotton Cultivars (Gossypium hirsutum L.) Grown in Xinjiang, China

2008 ◽  
Vol 34 (8) ◽  
pp. 1374-1380
Author(s):  
Tian-Zi CHEN ◽  
Shen-Jie WU ◽  
Fei-Fei LI ◽  
Wang-Zhen GUO ◽  
Tian-Zhen ZHANG
Keyword(s):  
Gossypium Hirsutum ◽  
In Vitro Regeneration ◽  
Gossypium Hirsutum L

scholarly journals The Effect of Improvised Media and Gelling Agents on In Vitro Germination of Cotton (Gossypium hirsutum. L.)

2016 ◽  
Vol 20 (3) ◽  
pp. 223-229
Author(s):  
Allah BAKHSH ◽  
Emine ANAYOL ◽  
Ayten Kübra TÜRKMEN ◽  
Sebahattin ÖZCAN
Keyword(s):  
Gossypium Hirsutum ◽  
In Vitro Germination ◽  
Gossypium Hirsutum L ◽  
Gelling Agents

Cytogenetic abnormalities in cotton (Gossypium hirsutum L.) cell cultures

Genome ◽  
1989 ◽  
Vol 32 (6) ◽  
pp. 1128-1134 ◽  
Author(s):  
Ruzhong Li ◽  
David M. Stelly ◽  
Norma L. Trolinder
Keyword(s):  
Gossypium Hirsutum ◽  
Cell Cultures ◽  
Single Cells ◽  
Metaphase Chromosomes ◽  
Gossypium Hirsutum L ◽  
Low Frequencies ◽  
Cytogenetic Abnormalities ◽  
Paired Samples ◽  
Ring Chromosomes

High frequencies of somaclonal phenotypic and cytogenetic variation have been observed previously among regenerants from cotton (Gossypium hirsutum L., 2n = 4x = 52). In this study we endeavored to determine if cytogenetic abnormalities would be detectable in cotton cell cultures and if so, whether or not the observed abnormalities would parallel those expected on the basis of previous cytogenetic analyses of cotton somaclones. Paired samples from suspension cultures established from 21-month-old 'Coker 312' and 8-month-old 'Coker 315' calli were pretreated or not pretreated with colchicine to detect cytogenetic abnormalities at metaphase or anaphase–telophase, respectively. Cell cultures established from both calli were found to vary in chromosome number. Hypoaneuploidy was common, but hyperaneuploidy and polyploidy were rare. Modal chromosome numbers for the 'Coker 312' and 'Coker 315' cultures were 46 and 50, respectively. Bridges at anaphase and telophase were frequent in the 'Coker 312' cultures but rare in the 'Coker 315'cultures. Cytogenetic differences between the cultures could be due to effects of culture age, genotype, their interaction, or other factors. Very small chromosomes, presumably centric fragments, as well as ring chromosomes and putative bridges between metaphase chromosomes occurred at low frequencies. The prevalence of hypoaneuploidy and rarity of hyperaneuploidy and polyploidy in cultures paralleled previous results on cotton somaclones, indicating that cytogenetic abnormalities arising in vitro probably contribute significantly to cotton somaclonal variation. The occurrence of hypoaneuploidy and bridges, including multiple bridges within single cells, is concordant with the hypothesis that breakage–fusion–bridge cycles may accumulate during in vitro culture of cotton.Key words: cotton, Gossypium, tissue culture, cytogenetics.

Wuschel overexpression promotes somatic embryogenesis and induces organogenesis in cotton (Gossypium hirsutum L.) tissues cultured in vitro

2013 ◽  
Vol 32 (5) ◽  
pp. 675-686 ◽  
Author(s):  
O. Bouchabké-Coussa ◽  
M. Obellianne ◽  
D. Linderme ◽  
E. Montes ◽  
A. Maia-Grondard ◽  
...  
Keyword(s):  
Somatic Embryogenesis ◽  
Gossypium Hirsutum ◽  
Gossypium Hirsutum L

Rapid in-vitro plant regeneration of cotton ( Gossypium hirsutum L.)

1998 ◽  
Vol 17 (4) ◽  
pp. 273-278 ◽  
Author(s):  
J. K. Hemphill ◽  
C. G. A. Maier ◽  
K. D. Chapman
Keyword(s):  
Plant Regeneration ◽  
Gossypium Hirsutum ◽  
In Vitro Plant Regeneration ◽  
Gossypium Hirsutum L ◽  
In Vitro Plant

scholarly journals In vitro plant regeneration of Iraqi cotton (Gossypium hirsutum L.) cultivars through embryonic axis

2014 ◽  
Vol 8 (2) ◽  
pp. 90-94
Author(s):  
Hussein Abdullah Ahmed ◽  
Mortaza Hajyzadeh ◽  
Surendra Barpete ◽  
Sebahattin Ozcan
Keyword(s):  
Plant Regeneration ◽  
Gossypium Hirsutum ◽  
Activated Charcoal ◽  
Embryonic Axis ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
In Vitro Plant Regeneration ◽  
Gossypium Hirsutum L ◽  
In Vitro Plant

In plant tissue culture studies, obtaining new plantlets from different parts of plant is a very important feature with direct or indirect ways of regeneration. The plant show different regeneration capacities from species to species. In this present study, in-vitro direct shoots development from embryonic axis of cotton (Gossypium hirsutum L. cv. Lashata) were comparatively studied. Embryonic axis, embryonic hypocotyl and plumule obtained from five days old in-vitro grown seeds. All explants were cultured on MS media supplemented with 1- 4 mg/l Benzyl amino purine (BAP) + 0.1 mg/l Naphthalene acetic acid (NAA) and 3 mg/l activated charcoal. Cultured explants were kept at growth chamber with photoperiod for 16 hours light and 8 hours dark, at 25oC. Reproducible in-vitro plant regeneration was obtained from embryonic axis when cultured onto MS medium supplemented 1 mg/l BAP + 0.1 mg/l NAA and 3 g/l Activated charcoal. Shoot induction was to be noted 53.3%, 36.7% and 12.3 in embryonic axis, plumule and embryonic hypocotyl respectively. All these shoots were capable of rooting on MS medium supplemented with 0.1 mg/l NAA, and establishing in soil 3-4 weeks.

Sign in / Sign up

Export Citation Format

Share Document