CO 2 accumulation in bioreactor suspension cultures of Cyclamen persicum Mill. and its effect on cell growth and regeneration of somatic embryos

1999 ◽  
Vol 18 (10) ◽  
pp. 863-867 ◽  
Author(s):  
A. Hohe ◽  
T. Winkelmann ◽  
H.-G. Schwenkel
Keyword(s):  
Cell Growth ◽  
Somatic Embryos ◽  
Suspension Cultures ◽  
Cyclamen Persicum

CYCLAMEN PERSICUM MILL. SOMATIC EMBRYOS DEVELOPED IN SUSPENSION CULTURES: HISTOLOGICAL ANALYSIS AND CONVERSION TO PLANT

2000 ◽  
pp. 83-90 ◽  
Author(s):  
B. Ruffoni ◽  
L. Semeria ◽  
P. Profumo ◽  
A. Bisio
Keyword(s):  
Somatic Embryos ◽  
Histological Analysis ◽  
Suspension Cultures ◽  
Cyclamen Persicum

The Influence of Organic and Inorganic Chemical Factors on Cell Growth and Anthraquinone Formation in Suspension Cultures of Galium vernum

1990 ◽  
Vol 186 (2) ◽  
pp. 117-124 ◽  
Author(s):  
J. Strobel ◽  
M. Hieke ◽  
E. Gebauer ◽  
E. Wind ◽  
D. Gröger
Keyword(s):  
Cell Growth ◽  
Suspension Cultures ◽  
Chemical Factors ◽  
Inorganic Chemical

Differentiation of somatic embryos from protoplasts isolated from embryogenic suspension cultures of Abies alba L.

1992 ◽  
Vol 11 (11) ◽  
Author(s):  
Sabine Hartmann ◽  
Hermann Lang ◽  
Gerhard Reuther
Keyword(s):  
Somatic Embryos ◽  
Abies Alba ◽  
Suspension Cultures ◽  
Embryogenic Suspension ◽  
Embryogenic Suspension Cultures

scholarly journals Bioproduction of Diosgenin in Callus Cultures of Balanites aegyptiaca: Effect of Growth Regulators, Explants and Somatic Embryogenesis

2006 ◽  
Vol 1 (3) ◽  
pp. 1934578X0600100
Author(s):  
Bishnu P. Chapagain ◽  
Vinod Saharan ◽  
Dan Pelah ◽  
Ram C. Yadav ◽  
Zeev Wiesman
Keyword(s):  
Somatic Embryogenesis ◽  
Growth Regulators ◽  
Embryogenic Callus ◽  
Somatic Embryos ◽  
Callus Formation ◽  
Basal Medium ◽  
Callus Cultures ◽  
Suspension Cultures ◽  
Balanites Aegyptiaca ◽  
Basal Media

This study describes the effects of plant growth regulators, explants, and somatic embryogenesis on in vitro production of the steroidal sapogenin, diosgenin, in callus cultures of the Balanites aegyptiaca (L.) Del.(desert date). Root, shoot, hypocotyl, and epicotyl callus culture of B. aegyptiaca, were raised on MS basal media supplemented with various combinations of either 2,4-D and NAA alone, or with BAP. The diosgenin content (on a dry weight basis) was found to be highest when calli were cultured in MS basal medium supplemented with 1.0 mg l−1 2,4-D alone and/or in combination with 0.5 mg l−1 BAP. However, the callus growth was highest in media supplemented with 2.5 or 3.0 mg l−1 2,4-D. MS basal media supplemented with 2,4-D 2.5 mg l−1 alone and in combination with 0.5 mg l−1 BAP induced pre-embryogenic callus formation on root cultures. When these pre-embryogenic callus cultures were used to establish cell suspension cultures, two growth densities were obtained in embryogenic suspension cultures, inducing clusters of somatic embryos at various stages of development. The maximum number of somatic embryos were obtained at the fifth week on the medium supplemented with 1.0 mg l−1 2,4-D. However, the diosgenin content in these somatic cells was found to be lower compared to the explant calluses. This study revealed that production of diosgenin in callus cultures of B. aegyptiaca is possible, but the amount is significantly affected by the growth regulators, type of explants, and somatic embryogenesis.

CRYOPRESERVATION OF EMBRYOGENIC SUSPENSION CULTURES OF CYCLAMEN PERSICUM MILL.

2006 ◽  
pp. 391-396 ◽  
Author(s):  
V. Mubmann ◽  
M. Serek ◽  
T. Winkelmann
Keyword(s):  
Suspension Cultures ◽  
Cyclamen Persicum ◽  
Embryogenic Suspension ◽  
Embryogenic Suspension Cultures

scholarly journals Enolases: storage compounds in seeds? Evidence from a proteomic comparison of zygotic and somatic embryos of Cyclamen persicum Mill.

2011 ◽  
Vol 75 (3) ◽  
pp. 305-319 ◽  
Author(s):  
Christina Rode ◽  
Sébastien Gallien ◽  
Dimitri Heintz ◽  
Alain Van Dorsselaer ◽  
Hans-Peter Braun ◽  
...  
Keyword(s):  
Somatic Embryos ◽  
Cyclamen Persicum ◽  
Storage Compounds

Calcium increases the yield of somatic embryos in carrot embryogenic suspension cultures

1990 ◽  
Vol 9 (4) ◽  
pp. 221-223 ◽  
Author(s):  
Marcel A. K. Jansen ◽  
Hilbert Booij ◽  
Jan H. N. Schel ◽  
Sacco C. de Vries
Keyword(s):  
Somatic Embryos ◽  
Suspension Cultures ◽  
Embryogenic Suspension ◽  
Embryogenic Suspension Cultures

scholarly journals Flavonoid Accumulation in Cell Suspension Cultures of Glycyrrhiza inflata Batal under Optimizing Conditions

2009 ◽  
Vol 64 (1-2) ◽  
pp. 68-72 ◽  
Author(s):  
Ying Yang ◽  
Feng He ◽  
Longjiang Yu ◽  
Jiaxing Ji ◽  
Yezhen Wang
Keyword(s):  
Cell Growth ◽  
Cell Suspension ◽  
Cell Cultures ◽  
Large Scale ◽  
Cell Suspension Cultures ◽  
Optimization Method ◽  
Suspension Cultures ◽  
Initial Inoculum ◽  
Nitrogen Concentrations ◽  
Glycyrrhiza Inflata

Cell growth and flavonoid production in cell suspension cultures of Glycyrrhiza inflata Batal were investigated under various initial inoculum densities, and sucrose and nitrogen concentrations to develop an optimization method for an improved flavonoid production. Both biomass accumulation and flavonoid production exhibited an “S” curve in one culture cycle, with the greatest value obtained on day 21, which showed that cell growth and fl avonoid biosynthesis went along isochronously. Moreover, according to the biomass and flavonoid production, the appreciate inoculum density, and the sucrose and nitrogen concentrations were 50 g FW L-1, 50 g L-1 and 120 mmol L-1, respectively. In addition, cell growth and flavonoid production showed a peak of 16.4 g DW L-1 and 95.7 mg L-1 on day 21 under the optimizing conditions, respectively. The flavonoid productivity of the cells which were cultured for 3 years is higher than that of the 3-year-old plant, which suggested that flavonoid production by cell cultures of G. inflata is a potentially profitable method. Therefore, this work is considered to be helpful for efficient large-scale bioprocessing of cell cultures in bioreactors.

Insect cell growth evaluation during serum-free adaptation in stirred suspension cultures

1997 ◽  
Vol 11 (2) ◽  
pp. 117-120 ◽  
Author(s):  
P.E. Cruz ◽  
J.L. Moreira ◽  
P.E. Cruz
Keyword(s):  
Cell Growth ◽  
Insect Cell ◽  
Suspension Cultures ◽  
Serum Free ◽  
Growth Evaluation
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