CYCLAMEN PERSICUM MILL. SOMATIC EMBRYOS DEVELOPED IN SUSPENSION CULTURES: HISTOLOGICAL ANALYSIS AND CONVERSION TO PLANT

2000 ◽  
pp. 83-90 ◽  
Author(s):  
B. Ruffoni ◽  
L. Semeria ◽  
P. Profumo ◽  
A. Bisio
Keyword(s):  
Somatic Embryos ◽  
Histological Analysis ◽  
Suspension Cultures ◽  
Cyclamen Persicum

CO 2 accumulation in bioreactor suspension cultures of Cyclamen persicum Mill. and its effect on cell growth and regeneration of somatic embryos

1999 ◽  
Vol 18 (10) ◽  
pp. 863-867 ◽  
Author(s):  
A. Hohe ◽  
T. Winkelmann ◽  
H.-G. Schwenkel
Keyword(s):  
Cell Growth ◽  
Somatic Embryos ◽  
Suspension Cultures ◽  
Cyclamen Persicum

scholarly journals Effects of the growth regulators for the induction of somatic embryos from explants in an endemic and threatened Echinocactus parryi Engelm.

2021 ◽  
Vol 27 (3) ◽  
pp. 431-447
Author(s):  
Dolores Adilene García-González ◽  
◽  
María del Socorro Santos-Díaz ◽  
Juan Pedro Flores-Margez ◽  
Pedro Osuna-Ávila ◽  
...  
Keyword(s):  
Growth Regulators ◽  
Somatic Embryos ◽  
Histological Analysis ◽  
Globular Stage ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Four Levels ◽  
Green Callus ◽  
Dichlorophenoxy Acetic Acid ◽  
Mature Seeds

Introduction: The list of threatened species is enhancing and needs to be revised by integrating plant tissue culture tools with conventional techniques that support the appropriate management of these species. Objective: To assess the effects of the growth regulators for the induction of somatic embryos from mature seeds, shoots, and compact green callus of Echinocactus parryi Engelm. and the histological analysis of the embryogenic structures. Materials and methods: A completely randomized design was utilized to evaluate three types of explants (apical, medium, and basal) cultured on basal Murashige & Skoog media (MS) with different growth regulators concentrations (2, 4-D [dichlorophenoxy acetic acid], BAP [6-benzylaminopurine] and kinetin, at four levels: 0.5, 1, 1.5, and 2 mg∙L -1 ). Histological analysis of the embryogenic structures was performed. Results and discussion: The 2, 4-D induced both embryogenic and organogenic callus from seeds and shoot explants. The globular stage did not evolve to their maturity, presumably because of 2, 4-D accumulation. The compact callus explants were the more efficient to induce 19.2 somatic embryos per explant when they were cultured in the medium with 0.5 mg∙L -1 kinetin. However, the latest phases did not germinate, probably due to abnormalities generated by genetic and epigenetic changes in the DNA that can cause abnormal somatic embryos. The histology image demonstrated that the globular and torpedo structures were visible under a microscope showing stained nucleus and numerous starch grains. Conclusions: E. parryi is a species that can produce a high number of embryogenic structures, which represents a great potential to grow massive plants.

Differentiation of somatic embryos from protoplasts isolated from embryogenic suspension cultures of Abies alba L.

1992 ◽  
Vol 11 (11) ◽  
Author(s):  
Sabine Hartmann ◽  
Hermann Lang ◽  
Gerhard Reuther
Keyword(s):  
Somatic Embryos ◽  
Abies Alba ◽  
Suspension Cultures ◽  
Embryogenic Suspension ◽  
Embryogenic Suspension Cultures

scholarly journals Bioproduction of Diosgenin in Callus Cultures of Balanites aegyptiaca: Effect of Growth Regulators, Explants and Somatic Embryogenesis

2006 ◽  
Vol 1 (3) ◽  
pp. 1934578X0600100
Author(s):  
Bishnu P. Chapagain ◽  
Vinod Saharan ◽  
Dan Pelah ◽  
Ram C. Yadav ◽  
Zeev Wiesman
Keyword(s):  
Somatic Embryogenesis ◽  
Growth Regulators ◽  
Embryogenic Callus ◽  
Somatic Embryos ◽  
Callus Formation ◽  
Basal Medium ◽  
Callus Cultures ◽  
Suspension Cultures ◽  
Balanites Aegyptiaca ◽  
Basal Media

This study describes the effects of plant growth regulators, explants, and somatic embryogenesis on in vitro production of the steroidal sapogenin, diosgenin, in callus cultures of the Balanites aegyptiaca (L.) Del.(desert date). Root, shoot, hypocotyl, and epicotyl callus culture of B. aegyptiaca, were raised on MS basal media supplemented with various combinations of either 2,4-D and NAA alone, or with BAP. The diosgenin content (on a dry weight basis) was found to be highest when calli were cultured in MS basal medium supplemented with 1.0 mg l−1 2,4-D alone and/or in combination with 0.5 mg l−1 BAP. However, the callus growth was highest in media supplemented with 2.5 or 3.0 mg l−1 2,4-D. MS basal media supplemented with 2,4-D 2.5 mg l−1 alone and in combination with 0.5 mg l−1 BAP induced pre-embryogenic callus formation on root cultures. When these pre-embryogenic callus cultures were used to establish cell suspension cultures, two growth densities were obtained in embryogenic suspension cultures, inducing clusters of somatic embryos at various stages of development. The maximum number of somatic embryos were obtained at the fifth week on the medium supplemented with 1.0 mg l−1 2,4-D. However, the diosgenin content in these somatic cells was found to be lower compared to the explant calluses. This study revealed that production of diosgenin in callus cultures of B. aegyptiaca is possible, but the amount is significantly affected by the growth regulators, type of explants, and somatic embryogenesis.

Ultrastructure of acridone alkaloid idioblasts in roots and cell cultures of Ruta graveolens

1986 ◽  
Vol 64 (6) ◽  
pp. 1089-1096 ◽  
Author(s):  
U. Eilert ◽  
B. Wolters ◽  
F. Constabel
Keyword(s):  
Cell Cultures ◽  
Histological Analysis ◽  
Suspension Cultures ◽  
Central Vacuole ◽  
Ruta Graveolens ◽  
Fungal Elicitors ◽  
Parenchyma Cells ◽  
Acridone Alkaloids ◽  
Acridone Alkaloid

Histological analysis of Ruta graveolens L. roots and in vitro grown cell suspensions revealed idioblasts with vacuoles containing clusters of droplets thought to be the storage compartment of acridone alkaloids. These idioblasts contained numerous vacuoles of varying sizes rather than the large, single, central vacuole characteristic of most adjacent parenchyma cells. The structure of idioblasts in roots and suspension cultures was identical. Treatment of suspension cultures with fungal elicitors known to increase alkaloid accumulation greatly did not affect the structure of idioblasts.

CRYOPRESERVATION OF EMBRYOGENIC SUSPENSION CULTURES OF CYCLAMEN PERSICUM MILL.

2006 ◽  
pp. 391-396 ◽  
Author(s):  
V. Mubmann ◽  
M. Serek ◽  
T. Winkelmann
Keyword(s):  
Suspension Cultures ◽  
Cyclamen Persicum ◽  
Embryogenic Suspension ◽  
Embryogenic Suspension Cultures

scholarly journals Enolases: storage compounds in seeds? Evidence from a proteomic comparison of zygotic and somatic embryos of Cyclamen persicum Mill.

2011 ◽  
Vol 75 (3) ◽  
pp. 305-319 ◽  
Author(s):  
Christina Rode ◽  
Sébastien Gallien ◽  
Dimitri Heintz ◽  
Alain Van Dorsselaer ◽  
Hans-Peter Braun ◽  
...  
Keyword(s):  
Somatic Embryos ◽  
Cyclamen Persicum ◽  
Storage Compounds

Calcium increases the yield of somatic embryos in carrot embryogenic suspension cultures

1990 ◽  
Vol 9 (4) ◽  
pp. 221-223 ◽  
Author(s):  
Marcel A. K. Jansen ◽  
Hilbert Booij ◽  
Jan H. N. Schel ◽  
Sacco C. de Vries
Keyword(s):  
Somatic Embryos ◽  
Suspension Cultures ◽  
Embryogenic Suspension ◽  
Embryogenic Suspension Cultures
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