Gene-based anchoring of the rat genetic linkage and cytogenetic maps: new regional localizations, orientation of the linkage groups, and insights into mammalian chromosome evolution

Two genetic linkage maps of mungbean derived from the cross Berken ACC 41 are reported. The F2 map constructed from 67 individuals consisted of 110 markers (52 RFLP and 56 RAPD) that grouped into 12 linkage groups. The linked markers spanned a total map distance of 758.3 cM. A recombinant inbred (RI) population derived from the 67 F2 individuals was used for the generation of an additional linkage map. The RI map, composed entirely of RAPD markers, consisted of 115 markers in 12 linkage groups. The linked markers spanned a total map distance of 691.7 cM. Using a framework set of RFLP markers, the F2 map was compared with another F2 mungbean map constructed in Minnesota. In general, the order of these markers was consistent between maps. Segregation distortion was observed for some markers. 14.5% (16/110) of mapped F2 markers and 24% (28/115) of mapped RI markers segregated with distorted ratios. Segregation distortion occurred in each successive generation after the F2 . The regions of distortion identified in the Australian maps did not coincide with regions of the Minnesota map.

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Translocation Breakpoints in Soybean Classical Genetic Linkage Groups 6 and 8

Crop Science ◽

10.2135/cropsci2003.1602 ◽

2003 ◽

Vol 43 (5) ◽

pp. 1602-1609 ◽

Cited By ~ 18

Author(s):

A. Assibi Mahama ◽

Reid G. Palmer

Keyword(s):

Genetic Linkage ◽

Linkage Groups ◽

Classical Genetic ◽

Translocation Breakpoints

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Genetic Linkage Map of the Edible BasidiomycetePleurotus ostreatus

Applied and Environmental Microbiology ◽

10.1128/aem.66.12.5290-5300.2000 ◽

2000 ◽

Vol 66 (12) ◽

pp. 5290-5300 ◽

Cited By ~ 61

Author(s):

Luis M. Larraya ◽

GÃ¯Â¿Â½mer PÃ¯Â¿Â½rez ◽

Enrique Ritter ◽

Antonio G. Pisabarro ◽

Lucı́a Ramı́rez

Keyword(s):

Linkage Map ◽

Genetic Linkage ◽

Gene Sequence ◽

Genetic Linkage Map ◽

Fragment Length Polymorphism ◽

Individual Cell ◽

Random Amplified Polymorphic Dna ◽

Rrna Gene ◽

Linkage Groups ◽

Rrna Gene Sequence

ABSTRACT We have constructed a genetic linkage map of the edible basidiomycete Pleurotus ostreatus (var. Florida). The map is based on the segregation of 178 random amplified polymorphic DNA and 23 restriction fragment length polymorphism markers; four hydrophobin, two laccase, and two manganese peroxidase genes; both mating type loci; one isozyme locus (est1); the rRNA gene sequence; and a repetitive DNA sequence in a population of 80 sibling monokaryons. The map identifies 11 linkage groups corresponding to the chromosomes ofP. ostreatus, and it has a total length of 1,000.7 centimorgans (cM) with an average of 35.1 kbp/cM. The map shows a high correlation (0.76) between physical and genetic chromosome sizes. The number of crossovers observed per chromosome per individual cell is 0.89. This map covers nearly the whole genome of P. ostreatus.

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A high-density genetic linkage map of a black spruce (Picea mariana) × red spruce (Picea rubens) interspecific hybrid

Genome ◽

10.1139/g10-099 ◽

2011 ◽

Vol 54 (2) ◽

pp. 128-143 ◽

Cited By ~ 10

Author(s):

Bum-Yong Kang ◽

John E. Major ◽

Om P. Rajora

Keyword(s):

Picea Mariana ◽

Interspecific Hybrid ◽

Genetic Linkage ◽

Genetic Linkage Map ◽

Black Spruce ◽

Picea Rubens ◽

Red Spruce ◽

Linkage Groups ◽

Genomic Resource ◽

Map Distance

Genetic maps provide an important genomic resource of basic and applied significance. Spruce ( Picea ) has a very large genome size (between 0.85 × 1010 and 2.4 × 1010 bp; 8.5–24.0 pg/1C, a mean of 17.7 pg/1C ). We have constructed a near-saturated genetic linkage map for an interspecific backcross (BC1) hybrid of black spruce (BS; Picea mariana (Mill.) B.S.P.) and red spruce (RS; Picea rubens Sarg.), using selectively amplified microsatellite polymorphic loci (SAMPL) markers. A total of 2284 SAMPL markers were resolved using 31 SAMPL–MseI selective nucleotide primer combinations. Of these, 1216 SAMPL markers showing Mendelian segregation were mapped, whereas 1068 (46.8%) SAMPL fragments showed segregation distortion at α = 0.05. Maternal, paternal, and consensus maps consistently coalesced into 12 linkage groups, corresponding to the haploid chromosome number (1n = 1x = 12) of 12 in the genus Picea. The maternal BS map consisted of 814 markers distributed over 12 linkage groups, covering 1670 cM, with a mean map distance of 2.1 cM between adjacent markers. The paternal BS × RS map consisted of 773 markers distributed over 12 linkage groups, covering 1563 cM, with a mean map distance of 2.0 cM between adjacent markers. The consensus interspecific hybrid BC1 map consisted of 1216 markers distributed over 12 linkage groups, covering 1865 cM (98% genome coverage), with a mean map distance of 1.5 cM between adjacent markers. The genetic map reported here provides an important genomic resource in Picea, Pinaceae, and conifers.

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A genetic linkage map for Stevia rebaudiana

Genome ◽

10.1139/g98-161 ◽

1999 ◽

Vol 42 (4) ◽

pp. 657-661 ◽

Cited By ~ 7

Author(s):

Y Yao ◽

M Ban ◽

J Brandle

Keyword(s):

Linkage Map ◽

Genetic Linkage ◽

Rapd Markers ◽

Genetic Linkage Map ◽

Average Distance ◽

Stevia Rebaudiana ◽

Linkage Groups ◽

Genome Map ◽

High Level ◽

Map Distance

To lay a foundation for molecular breeding efforts, the first genetic linkage map for Stevia rebaudiana has been constructed using segregation data from a pseudo test-cross F1 population. A total of 183 randomly amplified polymorphic DNA (RAPD) markers were analysed and assembled into 21 linkage groups covering a total distance of 1389 cM, with an average distance between markers of of 7.6 cM. The 11 largest linkage groups consisted of 4-19 loci, ranged in length from 56 to 174 cM, and accounted for 75% of the total map distance. Fifteen RAPD loci were found to be unlinked. From the 521 primers showing amplification products, 185 (35.5%) produced a total of 293 polymorphic fragments, indicating a high level of genetic diversity in stevia. Most of the RAPD markers in stevia segregated in normal Mendelian fashion.Key words: stevia, open-pollinated, genome map, RAPD.

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Integration of the Soybean Molecular and Classical Genetic Linkage Groups

Crop Science ◽

10.2135/cropsci1995.0011183x003500020027x ◽

1995 ◽

Vol 35 (2) ◽

pp. 436 ◽

Cited By ~ 75

Author(s):

R. C. Shoemaker ◽

J. E. Specht

Keyword(s):

Genetic Linkage ◽

Linkage Groups ◽

Classical Genetic

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Mapping of quantitative trait loci underlying resistance to cassava anthracnose disease

The Journal of Agricultural Science ◽

10.1017/s0021859615001057 ◽

2015 ◽

Vol 154 (7) ◽

pp. 1209-1217 ◽

Cited By ~ 3

Author(s):

A. BOONCHANAWIWAT ◽

S. SRAPHET ◽

S. WHANKAEW ◽

O. BOONSENG ◽

D. R. SMITH ◽

...

Keyword(s):

Quantitative Trait Loci ◽

Linkage Map ◽

Dna Markers ◽

Quantitative Trait ◽

Genetic Linkage ◽

Genetic Linkage Map ◽

Gene Annotation ◽

Linkage Groups ◽

Anthracnose Disease ◽

Trait Loci

SUMMARYCassava (Manihot esculenta Crantz) is an economically important root crop in Thailand, which is ranked the world's top cassava exporting country. Production of cassava can be hampered by several pathogens and pests. Cassava anthracnose disease (CAD) is an important disease caused by the fungus Colletotrichum gloeosporioides f. sp. manihotis. The pathogen causes severe stem damage resulting in yield reductions and lack of stem cuttings available for planting. Molecular studies of cassava response to CAD will provide useful information for cassava breeders to develop new varieties with resistance to the disease. The current study aimed to identify quantitative trait loci (QTL) and DNA markers associated with resistance to CAD. A total of 200 lines of two F1 mapping populations were generated by reciprocal crosses between the varieties Huabong60 and Hanatee. The F1 samples were genotyped based on simple sequence repeat (SSR) and expressed sequence tag-SSR markers and a genetic linkage map was constructed using the JoinMap®/version3·0 program. The results showed that the map consisted of 512 marker loci distributed on 24 linkage groups with a map length of 1771·9 centimorgan (cM) and a mean interval between markers of 5·7 cM. The genetic linkage map was integrated with phenotypic data for the response to CAD infection generated by a detached leaf assay test. A total of three QTL underlying the trait were identified on three linkage groups using the MapQTL®/version4·0 program. Those DNA markers linked to the QTL that showed high statistically significant values with the CAD resistance trait were identified for gene annotation analysis and 23 candidate resistance genes to CAD infection were identified.

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Development of a genetic linkage map for Sharon goatgrass (Aegilops sharonensis) and mapping of a leaf rust resistance gene

Genome ◽

10.1139/gen-2013-0065 ◽

2013 ◽

Vol 56 (7) ◽

pp. 367-376 ◽

Cited By ~ 8

Author(s):

P.D. Olivera ◽

A. Kilian ◽

P. Wenzl ◽

B.J. Steffenson

Keyword(s):

Linkage Map ◽

Leaf Rust ◽

Resistance Genes ◽

Genetic Linkage ◽

Genetic Linkage Map ◽

Chromosomal Location ◽

Dominant Gene ◽

Leaf Rust Resistance Gene ◽

Linkage Groups ◽

Aegilops Sharonensis

Aegilops sharonensis (Sharon goatgrass), a diploid wheat relative, is known to be a rich source of disease resistance genes for wheat improvement. To facilitate the transfer of these genes into wheat, information on their chromosomal location is important. A genetic linkage map of Ae. sharonensis was constructed based on 179 F2 plants derived from a cross between accessions resistant (1644) and susceptible (1193) to wheat leaf rust. The linkage map was based on 389 markers (377 Diversity Arrays Technology (DArT) and 12 simple sequence repeat (SSR) loci) and was comprised of 10 linkage groups, ranging from 2.3 to 124.6 cM. The total genetic length of the map was 818.0 cM, with an average interval distance between markers of 3.63 cM. Based on the chromosomal location of 115 markers previously mapped in wheat, the four linkage groups of A, B, C, and E were assigned to Ae. sharonensis (Ssh) and homoeologous wheat chromosomes 6, 1, 3, and 2. The single dominant gene (designated LrAeSh1644) conferring resistance to leaf rust race THBJ in accession 1644 was positioned on linkage group A (chromosome 6Ssh) and was flanked by DArT markers wpt-9881 (at 1.9 cM distal from the gene) and wpt-6925 (4.5 cM proximal). This study clearly demonstrates the utility of DArT for genotyping uncharacterized species and tagging resistance genes where pertinent genomic information is lacking.

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