Changes in acetylene reduction activities and effects of inoculated rhizosphere nitrogen-fixing bacteria on rice

2005 ◽  
Vol 41 (5) ◽  
pp. 371-378 ◽  
Author(s):  
Zhe Piao ◽  
Zongjun Cui ◽  
Bin Yin ◽  
Jian Hu ◽  
Chunhong Zhou ◽  
...  
Keyword(s):  
Acetylene Reduction ◽  
Nitrogen Fixing ◽  
Nitrogen Fixing Bacteria

Asymbiotic N2-fixing bacteria associated with three boreal conifers

1984 ◽  
Vol 14 (4) ◽  
pp. 595-597 ◽  
Author(s):  
L. Z. Florence ◽  
F. D. Cook
Keyword(s):  
Nitrogen Fixation ◽  
Acetylene Reduction ◽  
Nitrogen Fixing ◽  
Nitrogen Fixing Bacteria ◽  
Seedling Root ◽  
Natural Stands

Azotobacter spp., Azospirillum spp., and Desulfovibrio spp., were identified as the predominant nitrogen-fixing bacteria associated with seedling root sections collected from natural stands of Pinusbanksiana Lamb., Piceamariana (Mill.) B.S.P., and Larixlaricina (Du Roi) K. Koch growing in Alberta. Samples from the sandy upland habitat of P. banksiana exhibited lower average rates of nitrogen fixation relative to the wet lowland occupied by P. mariana and L. laricina. Average nitrogen-fixing capacity (by acetylene reduction) was greater among bacteria isolates from L. laricina than those from P. mariana. Azospirillum spp. were strongly associated with P. mariana, while Azotobacter spp. were isolated more frequently from L. laricina.

scholarly journals Nitrogen Fixation in Pozol, a Traditional Fermented Beverage

2020 ◽  
Vol 86 (16) ◽  
Author(s):  
Jocelin Rizo ◽  
Marco A. Rogel ◽  
Daniel Guillén ◽  
Carmen Wacher ◽  
Esperanza Martinez-Romero ◽  
...  
Keyword(s):  
Nitrogen Fixation ◽  
Acetylene Reduction ◽  
Growth Promotion ◽  
Point Of View ◽  
Intestinal Microbiome ◽  
Acetylene Reduction Activity ◽  
Nitrogen Fixing ◽  
Nitrogen Fixing Bacteria ◽  
Free Living ◽  
Functional Perspective

ABSTRACT Traditional fermentations have been widely studied from the microbiological point of view, but little is known from the functional perspective. In this work, nitrogen fixation by free-living nitrogen-fixing bacteria was conclusively demonstrated in pozol, a traditional Mayan beverage prepared with nixtamalized and fermented maize dough. Three aspects of nitrogen fixation were investigated to ensure that fixation actually happens in the dough: (i) the detection of acetylene reduction activity directly in the substrate, (ii) the presence of potential diazotrophs, and (iii) an in situ increase in acetylene reduction by inoculation with one of the microorganisms isolated from the dough. Three genera were identified by sequencing the 16S rRNA and nifH genes as Kosakonia, Klebsiella, and Enterobacter, and their ability to fix nitrogen was confirmed. IMPORTANCE Nitrogen-fixing bacteria are found in different niches, as symbionts in plants, in the intestinal microbiome of several insects, and as free-living microorganisms. Their use in agriculture for plant growth promotion via biological nitrogen fixation has been extensively reported. This work demonstrates the ecological and functional importance that these bacteria can have in food fermentations, reevaluating the presence of these genera as an element that enriches the nutritional value of the dough.

A Survey of Asymbiotic Nitrogen Fixation in the Rhizosphere of Weeds

Weed Science ◽  
1978 ◽  
Vol 26 (2) ◽  
pp. 148-150 ◽  
Author(s):  
A. R. Conklin ◽  
P. K. Biswas
Keyword(s):  
Thin Film ◽  
Nitrogen Fixation ◽  
Acetylene Reduction ◽  
Rhizosphere Soil ◽  
Soil Samples ◽  
Reduction Technique ◽  
Nitrogen Fixing ◽  
Weed Species ◽  
Nitrogen Fixing Bacteria ◽  
Asymbiotic Nitrogen Fixation

The occurrence of asymbiotic nitrogen fixing bacteria in the rhizosphere of twenty weed species has been investigated. Using the roll tube method root-rhizosphere soil samples were diluted and added to test tubes containing low N media. Tubes were flushed with nitrogen, stoppered, and rolled to form a thin film of media on the inner wall. After 7 days incubation the nitrogen fixation rates were estimated using the acetylene reduction technique. Rates ranged from 38 to 783 nmoles acetylene reduced per hour. Of twenty species tested, three showed high rhizosphere populations of asymbiotic nitrogen fixing bacteria.

Use of Pankhurst tubes to assay acetylene reduction by facultative and anaerobic nitrogen-fixing bacteria

1969 ◽  
Vol 15 (11) ◽  
pp. 1342-1343 ◽  
Author(s):  
N. E. R. Campbell ◽  
Harold J. Evans
Keyword(s):  
Nitrogen Fixation ◽  
Acetylene Reduction ◽  
Most Probable Number ◽  
Bacterial Isolates ◽  
Nitrogen Fixing ◽  
Nitrogen Fixing Bacteria ◽  
Probable Number ◽  
Density Measurements ◽  
Large Numbers ◽  
Root Surfaces

Pankhurst tubes are simple and inexpensive vessels in which to screen large numbers of bacterial isolates by the acetylene reduction test for nitrogen fixation. A modification is described which allows, in addition, optical density measurements of growth. The method is well suited to most probable number estimations of facultative and anaerobic nitrogen-fixing bacteria in soils and on root surfaces.

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