DNA damage in the early primordial anther is closely correlated with stamen arrest in the female flower of cucumber ( Cucumis sativus L.)

Planta ◽  
2003 ◽  
Vol 217 (6) ◽  
pp. 888-895 ◽  
Author(s):  
Yu-Jin Hao ◽  
Dong-Hui Wang ◽  
Yi-Ben Peng ◽  
Su-Lan Bai ◽  
Li-Yun Xu ◽  
...  
2020 ◽  
Vol 8 (1) ◽  
pp. 53-59
Author(s):  
Le Thi Kim Ngoc ◽  
Nguyen Tran Dong Phuong

Haploid plants achieve through androgenesis or gynogenesis. In gynogenesis method, the ovary or ovule are used as explants induct haploid plants. Female flower one day before flowering of Cucumis sativus L. are collected. Cold pretreatment of ovaries at 4°C up to 24 hours and culture under dark conditions. Significantly enhanced callus induction response is compared with cultures under 4-week cultured on CBM medium supplemented with various concentration of TDZ 0.01-0.04 mg/L. After 4 weeks, ovaries are transferred to medium with kinetin 0.05 – 0.20 mg/L. Then, ovaries were transferred to medium supplemented with BA: IAA 3:1. Finally, green ovaries were transferred to BA 1.5 mg/L and GA3 1.5 mg/L. The results showed that ovary induction has best affected on CBM with TDZ 0.03 mg/L with 11 callus/sample. Ovaries developed on kinetin 0.1 mg/L with 7.4 callus/sample. Ovaries become green and had leaves and roots formation on BA: IAA (3 mg/L: 1 mg/L). 11 plantlets were harvested from ovary culture after 12-week culture on CBM supplemented with BA 1.5 mg/L and GA3 1.5 mg/L.


2017 ◽  
Vol 2 (02) ◽  
pp. 130-135
Author(s):  
V. Singh ◽  
K. N. Shah ◽  
D. K. Rana

Thirteen strains of cucumber (Cucumis sativus L.) were evaluated at Horticultural Research Centre, Department of Horticulture, H.N.B. Garhwal University, Srinagar (Garhwal), Uttarakhand (India) to study the variability, heritability and genetic advance for growth, yield, quality and seed parameters. The analysis of variance revealed highly significant differences for almost all the characters. The perusal of present investigation indicated that the values of phenotypic coefficient of variation (PCV) were higher than the genotypic coefficients of variation (GCV) for all characters studied. The genotypic coefficients of variation and phenotypic coefficients of variation were moderate to low for all the characters except number of nodes bearing first male flower (33.02%, 33.66%). The high magnitude of heritability high estimated genetic advance over mean were found highest for length of fruit (100%, 58.40%), weight of fruit (99%, 39.92%), length of vine (98%, 36.12%), number of seeds per fruit (98%,50.16%), number of nodes per vine (97%, 53.57%), number of fruits per vine (97%, 44.33%), number of nodes bearing first male flower (96%, 66.74%), TSS (96%, 44.25%), calcium content (95%, 21.75%), number of nodes bearing first female flower (94%, 58.36%), duration of harvesting (93%, 36.04%), total fruit yield per vine (92%,35.93%) and diameter of fruit (92%, 35.80%) thus indicating that these characters had additive gene effect and therefore, they are more reliable for effective selection


HortScience ◽  
1990 ◽  
Vol 25 (9) ◽  
pp. 1063C-1063

Hybrid seed of cucumber (Cucumis sativus) is expensive to produce. Production of `artificial seeds' through somatic embryogenesis may be a viable alternative. Somatic embryos were induced, multiplied on a semisolid medium for 8-10 weeks, and germinated on agar-gelled medium before transplanting to soil. It was then important to determine the extent of variation among plants derived from somatic embryos. The criteria for variation among regenerants of cultivar Clinton were; plant height, fruit shape, fruit weight and number (yield/plant), days to first female flower and variation in selected isozymes. All measurements were taken on greenhouse-grown plants. Some regenerants of Clinton were also planted in the field and they flowered and, qualitatively, bore fruit as well as the zygote-derived plants. When quantitative measurements were made, variation was greater than for plants from zygotic embryos, but the visual impact was that there was little variation amongst regenerants. Regenerants grew more slowly and tended to yield higher numbers of slightly smaller fruits than plants from zygotic embryos. Average yield per plant was higher for somatic embryo-derived plants. For cultivar Corona only morphology of plant and fruit was examined. One plant was especially visually mutant and unacceptable as a commercial plant. The defects were readily identifiable in the seedling stage. Preliminary evidence suggests that `artificial seeds' of cucumbers may be a viable proposition.


2021 ◽  
Vol 21 (no 1) ◽  
Author(s):  
S. Fowmina Sulaiha ◽  
A. Anburani ◽  
A. Anburani ◽  
A. Anburani

A field experiment was carried out to study the “Effect of organic manures and biofortification of selenium on physiological and flowering parameters of cucumber (Cucumis sativus L.). The study was conducted during two seasons viz., Season I (FebruaryMay 2019) and Season II (July-October 2019). The experiment was carried out following the principles of randomized block design with fifteen treatments which were replicated thrice. The organic manures used in the experiment were farmyard manure (25 t ha-1), enriched manure (1 t ha-1) along with consortium of biofertilizers (2 kg ha-1). The selenium in the form of sodium selenate (Na2 SeO4 ) was used at varying concentrations (5, 10 and 20 µg L-1) as soil and foliar application. The physiological parameters viz.,chlorophyll content index and dry matter production per plant were recorded the highest in which EM @ 1 t ha -1 + CBF @ 2 kg ha -1 + Se (20 µg L -1) as foliar was applied. The flowering parameters viz., days to first male flower appearance, node number of first male flower, number of male flowers per vinewere found to be minimum in the treatment that received FYM @ 25 t ha-1 + CBF @ 2 kg ha-1 + Se (20 µg L-1) as foliar application. The days to first female flower appearance, node number of first female flower, number of female flowers per vine, fruit set percentage andsex ratio were observed to be superior in the treatment that received EM @ 1 t ha-1 + CBF @ 2 kg ha-1 + Se (20 µg L-1) as foliar application in both the seasons respectively.


2015 ◽  
Vol 1 (1) ◽  
pp. 24-34
Author(s):  
Muhammad Maulana Malikul Ikram ◽  
◽  
Rizkita Rachmi Esyanti ◽  
Ahmad Faizal ◽  
◽  
...  

HortScience ◽  
1990 ◽  
Vol 25 (9) ◽  
pp. 1063c-1063 ◽  

Hybrid seed of cucumber (Cucumis sativus) is expensive to produce. Production of `artificial seeds' through somatic embryogenesis may be a viable alternative. Somatic embryos were induced, multiplied on a semisolid medium for 8-10 weeks, and germinated on agar-gelled medium before transplanting to soil. It was then important to determine the extent of variation among plants derived from somatic embryos. The criteria for variation among regenerants of cultivar Clinton were; plant height, fruit shape, fruit weight and number (yield/plant), days to first female flower and variation in selected isozymes. All measurements were taken on greenhouse-grown plants. Some regenerants of Clinton were also planted in the field and they flowered and, qualitatively, bore fruit as well as the zygote-derived plants. When quantitative measurements were made, variation was greater than for plants from zygotic embryos, but the visual impact was that there was little variation amongst regenerants. Regenerants grew more slowly and tended to yield higher numbers of slightly smaller fruits than plants from zygotic embryos. Average yield per plant was higher for somatic embryo-derived plants. For cultivar Corona only morphology of plant and fruit was examined. One plant was especially visually mutant and unacceptable as a commercial plant. The defects were readily identifiable in the seedling stage. Preliminary evidence suggests that `artificial seeds' of cucumbers may be a viable proposition.


2018 ◽  
Vol 17 (1) ◽  
pp. 127-138
Author(s):  
Priyanka Pal ◽  
Kuldeep Yadav ◽  
Krishan Kumar ◽  
Narender Singh

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