scholarly journals Comparative in situ analyses of cell wall matrix polysaccharide dynamics in developing rice and wheat grain

Planta ◽  
2014 ◽  
Vol 241 (3) ◽  
pp. 669-685 ◽  
Author(s):  
Richard Palmer ◽  
Valérie Cornuault ◽  
Susan E. Marcus ◽  
J. Paul Knox ◽  
Peter R. Shewry ◽  
...  
Keyword(s):  
Cell Wall ◽  
Wheat Grain ◽  
Cell Wall Matrix

In vitro and in vivo polysaccharides assembly: ultrastructural and cytochemical study of growing plant cell wall components.

1978 ◽  
Vol 36 (2) ◽  
pp. 434-435
Author(s):  
D. Reis ◽  
B. Vian ◽  
J. C. Roland
Keyword(s):  
Cell Wall ◽  
Self Assembly ◽  
Plant Cell Wall ◽  
Higher Plants ◽  
Three Dimensional ◽  
Cytochemical Study ◽  
Wall Components

Wall morphogenesis in higher plants is a problem still open to controversy. Until now the possibility of a transmembrane control and the involvement of microtubules were mostly envisaged. Self-assembly processes have been observed in the case of walls of Chlamydomonas and bacteria. Spontaneous gelling interactions between xanthan and galactomannan from Ceratonia have been analyzed very recently. The present work provides indications that some processes of spontaneous aggregation could occur in higher plants during the formation and expansion of cell wall.Observations were performed on hypocotyl of mung bean (Phaseolus aureus) for which growth characteristics and wall composition have been previously defined.In situ, the walls of actively growing cells (primary walls) show an ordered three-dimensional organization (fig. 1). The wall is typically polylamellate with multifibrillar layers alternately transverse and longitudinal. Between these layers intermediate strata exist in which the orientation of microfibrils progressively rotates. Thus a progressive change in the morphogenetic activity occurs.

GPC‐ELISA method for systematic mass distribution profiling of plant cell wall matrix polysaccharides

2021 ◽  
Author(s):  
Sukhita Sathitnaitham ◽  
Anongpat Suttangkakul ◽  
Passorn Wonnapinij ◽  
Simon J. McQueen‐Mason ◽  
Supachai Vuttipongchaikij
Keyword(s):  
Cell Wall ◽  
Mass Distribution ◽  
Plant Cell ◽  
Plant Cell Wall ◽  
Elisa Method ◽  
Cell Wall Matrix

Characterization of the cell wall matrix polysaccharides and glycoside-hydrolyzing enzymes of Distylium racemosum callus

Plant Science ◽  
2010 ◽  
Vol 178 (2) ◽  
pp. 213-220 ◽  
Author(s):  
Haruyoshi Konno ◽  
Hisaaki Tsumuki ◽  
Susumu Nakashima
Keyword(s):  
Cell Wall ◽  
Cell Wall Matrix ◽  
Hydrolyzing Enzymes

The graft framework: Quantitative changes in cell wall matrix polysaccharides throughout the tomato graft union formation

2021 ◽  
pp. 118781
Author(s):  
Carlos Frey ◽  
Alba Manga-Robles ◽  
José Luis Acebes ◽  
Antonio Encina
Keyword(s):  
Cell Wall ◽  
Union Formation ◽  
Graft Union ◽  
Cell Wall Matrix ◽  
Quantitative Changes

Effects of Drying and Ensiling on In situ Cell Wall Degradation Kinetics of Tomato Pomace in Ruminant

2012 ◽  
Vol 6 (4) ◽  
pp. 196-202 ◽  
Author(s):  
Naser Maheri-Sis ◽  
Mohammad Chamani ◽  
Ali Asghar Sadeghi ◽  
Ali Mirzaaghaz ◽  
Kambiz Nazeradl ◽  
...  
Keyword(s):  
Cell Wall ◽  
Degradation Kinetics ◽  
Cell Wall Degradation ◽  
Tomato Pomace ◽  
Kinetics Of

Visualization of sporopollenin-containing pathogenic green micro-alga Prototheca wickerhamii by fluorescent in situ hybridization (FISH)

2009 ◽  
Vol 55 (4) ◽  
pp. 465-472 ◽  
Author(s):  
Ryohei Ueno
Keyword(s):  
Cell Wall ◽  
In Situ Hybridization ◽  
Cell Walls ◽  
Fluorescent In Situ Hybridization ◽  
Rapid Identification ◽  
Oligonucleotide Probes ◽  
Logarithmic Growth Phase ◽  
Prototheca Wickerhamii ◽  
Algal Genus

Fluorescent in situ hybridization (FISH) using taxon-specific, rRNA-targeted oligonucleotide probes is one of the most powerful tools for the rapid identification of harmful microorganisms. However, eukaryotic algal cells do not always allow FISH probes to permeate over their cell walls. Members of the pathogenic micro-algal genus Prototheca are characterized by their distinctive cell-wall component, sporopollenin, an extremely tough biopolymer that resists acid and alkaline hydrolysis, enzyme attack, and acetolysis. To our knowledge, there has been no report of the successful permeation by the oligonucleotide probes over the cell walls of unicellular green micro-algae, which contain sporopollenin. The DNA probes passed through the cell wall of Prototheca wickerhamii after treating the algal cells with cetyltrimethylammonium bromide (CTAB). Most cells in the middle logarithmic growth phase culture fluoresced when hybridized with the rRNA-targeted universal probe for eukaryotes, though individual cells included in this culture differed in the level of cell-wall vulnerability to attack by the polysaccharide-degrading enzyme, thus reflecting the different stages of the life cycle. This is the first report regarding the visualization of sporopollenin-containing, green micro-algal cells by FISH.

The elastic modulus of spruce wood cell wall material measured by an in situ bending technique

2006 ◽  
Vol 41 (16) ◽  
pp. 5122-5126 ◽  
Author(s):  
Steffen Orso ◽  
Ulrike G. K. Wegst ◽  
Eduard Arzt
Keyword(s):  
Cell Wall ◽  
Elastic Modulus ◽  
Wood Cell Wall ◽  
Wall Material ◽  
Cell Wall Material ◽  
Spruce Wood

Viscoelastic properties of woody hemp core

Holzforschung ◽  
2011 ◽  
Vol 65 (2) ◽  
Author(s):  
Rahime Bag ◽  
Johnny Beaugrand ◽  
Patrice Dole ◽  
Bernard Kurek
Keyword(s):  
Cell Wall ◽  
Viscoelastic Properties ◽  
Specific Effect ◽  
Mechanical Analysis ◽  
Low Molecular Weight ◽  
Dielectric Analysis ◽  
Technological Transformation ◽  
Chain Mobility ◽  
Cell Wall Polymers

Abstract The aim of this study was to determine the effect of removing extractives from the woody core of hemp (chènevotte) on the chain mobility of hemicelluloses and lignins, which can react during technological transformation such as de-fibering and/or composite materials production. Extractives are molecules with low molecular weight, which are present in the cell wall matrix and can be readily removed by solvents. In the present paper, the nature and amounts of extractives, removed under different conditions and with solvents of different polarities, were determined. The mobility and structural relaxations of lignins and hemicelluloses were stu-died in situ by dynamic mechanical analysis and dielectric analysis under controlled moisture content. Extractions at low temperature led to rigidification of lignins and plasticizing of hemicelluloses, probably due to local changes by the selective removal of molecules interacting with the polymers. Probably, the accessibility of hemicelluloses to plasticizing water was increased at controlled humidity. In contrast, hot extractions including water induced rigidification of the hemi-celluloses and plasticizing of lignins. This could be related to a combination of molecule extractions and chemical modi-fications of both polymers. This interpretation is supported by the variation of activation energy for relaxation of hemi-celluloses. It can be concluded that each type of extraction has a clear specific effect on the relaxation properties of the amorphous cell wall polymers.

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