Taxonomic positions of four Gram-negative bacterial strains, which were isolated from larvae of two insects in Jeju, Republic of Korea, were determined by a polyphasic approach. Strains CWB-B4, CWB-B41 and CWB-B43 were recovered from larvae of Protaetia brevitarsis seulensis, whereas strain BWR-B9T was from larvae of Allomyrina dichotoma. All the isolates grew at 10–37 °C, at pH 5.0–9.0 and in the presence of 4 % (w/v) NaCl. The 16S rRNA gene phylogeny showed that the four isolates formed two distinct sublines within the order
Enterobacteriales
and closely associated with members of the genus
Jinshanibacter
. The first group represented by strain CWB-B4 formed a tight cluster with
Jinshanibacter xujianqingii
CF-1111T (99.3 % sequence similarity), whereas strain BWR-B9T was most closely related to
Jinshanibacter zhutongyuii
CF-458T (99.5 % sequence similarity). The 92 core gene analysis showed that the isolates belonged to the family
Budviciaceae
and supported the clustering shown in 16S rRNA gene phylogeny. The genomic DNA G+C content of the isolates was 45.2 mol%. A combination of overall genomic relatedness and phenotypic distinctness supported that three isolates from Protaetia brevitarsis seulensis are different strains of
Jinshanibacter xujianqingii
, whereas one isolate from Allomyrina dichotoma represents a new species of the genus
Jinshanibacter
. On the basis of results obtained here, Jinshanibacter allomyrinae sp. nov. (type strain BWR-B9T=KACC 22153T=NBRC 114879T) and Insectihabitans xujianqingii gen. nov., comb. nov. are proposed, with the emended descriptions of the genera
Jinshanibacter
,
Limnobaculum
and
Pragia
.
A Six Nuclear Gene Phylogeny of Citrus (Rutaceae) Taking into Account Hybridization and Lineage Sorting
