Molecular Insights into the Assembly and Functional Diversification of Typhoid Toxin

Typhoid fever is a devastating disease that kills more than 115,000 people every year and is caused by Salmonella Typhi. Typhoid toxin, exclusively produced by S .

Attractiveness of Central Public Spaces in Small Polish Towns Based on a Spatial Order Analysis

The purpose of this article is to evaluate the attractiveness of centrally located public spaces (main squares) in select new small towns in Poland. The evaluation was conducted from the spatial order perspective. Spatial order is composed of five elements: architectural and urban planning, functional, aesthetic, social, and “green” orders. The new small towns included in this analysis are settlement units, which in 2020 were populated by up to 20,000 inhabitants and received municipal rights in the 21st century. We used the point bonitation method in our research based on the source material collected during a field study. A total of 286 inventory cards of buildings and nine cards of town squares were compiled. The analysis demonstrated that the main squares in the towns studied are characterised by low or average levels of attractiveness from the spatial order perspective. The architectural–urban planning order in the towns in question was related to the number of inhabitants as well as the period over which a given settlement unit had municipal rights. A larger number of inhabitants had a positive influence on the functional diversification of the central squares and their development, whereas a small number limited both the functional diversification and the number of small architectural elements found at the square. The social order in the given towns was not connected to the number of inhabitants. The elements of social order were assessed favourably, both in larger towns that revitalised their central squares and in smaller settlements. The aesthetic and green orders were strongly related to the revitalisation of public space.

Two Is Company, but Four Is a Party—Challenges of Tetraploidization for Cell Wall Dynamics and Efficient Tip-Growth in Pollen

Some cells grow by an intricately coordinated process called tip-growth, which allows the formation of long tubular structures by a remarkable increase in cell surface-to-volume ratio and cell expansion across vast distances. On a broad evolutionary scale, tip-growth has been extraordinarily successful, as indicated by its recurrent ‘re-discovery’ throughout evolutionary time in all major land plant taxa which allowed for the functional diversification of tip-growing cell types across gametophytic and sporophytic life-phases. All major land plant lineages have experienced (recurrent) polyploidization events and subsequent re-diploidization that may have positively contributed to plant adaptive evolutionary processes. How individual cells respond to genome-doubling on a shorter evolutionary scale has not been addressed as elaborately. Nevertheless, it is clear that when polyploids first form, they face numerous important challenges that must be overcome for lineages to persist. Evidence in the literature suggests that tip-growth is one of those processes. Here, I discuss the literature to present hypotheses about how polyploidization events may challenge efficient tip-growth and strategies which may overcome them: I first review the complex and multi-layered processes by which tip-growing cells maintain their cell wall integrity and steady growth. I will then discuss how they may be affected by the cellular changes that accompany genome-doubling. Finally, I will depict possible mechanisms polyploid plants may evolve to compensate for the effects caused by genome-doubling to regain diploid-like growth, particularly focusing on cell wall dynamics and the subcellular machinery they are controlled by.

Urban Development and the (Re)use of the Communist-Built Industrial and Agricultural Sites after 1990. The Showcase of Bucharest–Ilfov Development Region

Urban development and changing the patterns of industry and agriculture had caused the foremost spatial and functional transformations of the post-communist period in Romania. These changes have resulted in increased land consumption, often including the reuse of abandoned or non-residential built-up areas (e.g., industrial, agricultural). By integrating spatial and statistical data, the current analysis has revealed as key features: spatial shrinkage, fragmentation, functional diversification, tertiarization and change of patterns. Using a functional change matrix, five main (re)use types have been identified and quantified: maintenance, conversion, replacement, abandonment, and demolition. Overall, between 1990 and 2018, over 50% functional losses have been recorded.