Cellular and subcellular localization of SALMFamide (S1)-like immunoreactivity within the central nervous system of the nematode Ascaris suum (Nematoda, Ascaroidea)

1996 ◽  
Vol 82 (2) ◽  
pp. 149-156 ◽  
Author(s):  
D. J. A. Brownlee ◽  
I. Fairweather ◽  
M. C. Thorndyke ◽  
C. F. Johnston
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Subcellular Localization ◽  
Ascaris Suum ◽  
The Central Nervous System

SUBCELLULAR LOCALIZATION OF 5-HYDROXY- TRYPTAMINE IN THE CENTRAL NERVOUS SYSTEM OF LAMELLIBRANCHIATES

1965 ◽  
Vol 12 (4) ◽  
pp. 245-251 ◽  
Author(s):  
I. Zs.-NAGY ◽  
KATALIN S.-RÓZSA ◽  
J. SALÁNKI ◽  
I. FÖLDES ◽  
L. PERÉNYI ◽  
...  
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Subcellular Localization ◽  
The Central Nervous System

Immunocytochemical demonstration of a neuropeptide in Ascaris suum (Nematoda) using an antiserum to FMR Famide

Parasitology ◽  
1988 ◽  
Vol 97 (1) ◽  
pp. 81-88 ◽  
Author(s):  
T. R. B. Davenport ◽  
D. L. Lee ◽  
R. E. Isaac
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Peripheral Nervous System ◽  
Ascaris Suum ◽  
Nerve Ring ◽  
Immunocytochemical Technique ◽  
The Central Nervous System ◽  
Nerve Cords

SUMMARYA FMRFamide-like peptide has been detected in the nematode Ascaris suum, using the peroxidase-anti-peroxidase (PAP) immunocytochemical technique. Positive reactions were obtained in both the central nervous system and the peripheral nervous system of the worm, the strongest reactions being in the anterior nerve ring, the cephalic papillary ganglia, the lateral ganglia and the dorso-rectal ganglion. Immunoreactivity was observed along the length of the main nerve cords of the worm and, to a lesser extent, in the pharyngeal nerve cords. The possible role of this neuropeptide in the physiology of the nematode is discussed.

Immunocytochemical demonstration of neuropeptides in the central nervous system of the roundworm, Ascaris suum (Nematoda: Ascaroidea)

Parasitology ◽  
1993 ◽  
Vol 106 (3) ◽  
pp. 305-316 ◽  
Author(s):  
D. J. A. Brownlee ◽  
I. Fairweather ◽  
C. F. Johnston ◽  
D. Smart ◽  
C. Shaw ◽  
...  
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Peptide Yy ◽  
Ascaris Suum ◽  
Nerve Ring ◽  
Gonadotropin Releasing Hormone ◽  
Releasing Hormone ◽  
Indirect Immunofluorescence Technique ◽  
The Central Nervous System ◽  
Nerve Cords

SUMMARYThe localization and distribution of neuropeptides in the central nervous system of the pig roundworm, Ascaris suum, have been determined by an indirect immunofluorescence technique in conjunction with confocal microscopy. Antisera to 25 vertebrate peptides and two invertebrate peptides were used to screen the worm for immunoreactivity (IR). Immuno-staining was obtained with antisera to pancreatic polypeptide (PP), peptide YY (PYY), neuropeptide Y (NPY), gastrin, cholecystokinin (CCK), substance P (SP), atrial natriuretic peptide (ANP), salmon gonadotropin-releasing hormone (SGnRH), mammalian gonadotropin-releasing hormone (MGnRH), chromogranin A (CGA) and FMRFamide. The most extensive patterns of IR occurred with antisera to PYY, FMRFamide and gastrin. IR was evident in nerve cells and fibres in the ganglia associated with the anterior nerve ring and in the main nerve cords and their commissures; IR to FMRFamide also occurred in the posterior nerve ring. Immunostaining for the other peptides was confined to the nerve cords, with the number of immunoreactive nerve fibres varying from peptide to peptide.

Histochemical mapping of NADPH diaphorase in the nervous system of the parasitic nematode Ascaris suum

Parasitology ◽  
1995 ◽  
Vol 110 (5) ◽  
pp. 625-637 ◽  
Author(s):  
Z. A. Bascal ◽  
A. Montgomery ◽  
L. Holden-Dye ◽  
R. G. Williams ◽  
R. J. Walker
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Parasitic Nematode ◽  
Ascaris Suum ◽  
No Synthase ◽  
Nadph Diaphorase ◽  
Positive Staining ◽  
Ventral Ganglion ◽  
Intense Staining ◽  
The Central Nervous System

SUMMARYNADPH diaphorase has recently been discovered to be responsible for neuronal nitric oxide (NO) synthase activity in mammals. It thus serves as a histochemical marker for the localization of NO synthase in the nervous system. The histochemical technique was used to map out potential NO-producing neurones in the nervous system of the parasitic nematode, Ascaris suum. Positive staining for NADPH diaphorase was present in various parts of the central nervous system, in particular within selective cell bodies and fibres in the ventral ganglion, the retrovesicular ganglion, ventral and dorsal cords and sublateral lines. Intense staining was also present in the motorneurone commissures, indicating a potential role for NO as a neurotransmitter at the neuromuscular junction. NADPH disphorase-positive neurones were not confined to the central nervous system. Selective staining was also present in the enteric nervous system, in particular the pharynx and in the peripheral nervous system innervating the sensory organs.

Ultrastructural localization of pancreatic polypeptide- and FMRFamide immunoreactivities within the central nervous system of the nematode, Ascaris suum (Nematoda: Ascaroidea)

Parasitology ◽  
1994 ◽  
Vol 108 (5) ◽  
pp. 587-593 ◽  
Author(s):  
D. J. A. Brownlee ◽  
G. P. Brennan ◽  
D. W. Halton ◽  
I. Fairweather ◽  
C. Shaw
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Pancreatic Polypeptide ◽  
Ascaris Suum ◽  
Ultrastructural Localization ◽  
Cross Reactivity ◽  
Double Labelling ◽  
Nerve Terminals ◽  
The Central Nervous System ◽  
Gold Labelling

SummaryA post-embedding immunogold technique has been used to examine the subcellular distribution of immunoreactivities to vertebrate pancreatic polypeptide (PP) and to the invertebrate peptide, FMRFamide within the central nervous system (CNS) of the nematode, Ascaris suum. Gold labelling of peptide was localized exclusively over dense-cored vesicles within nerve cell bodies, nerve axons and nerve terminals of the main ganglia and nerve cords in the CNS. Double-labelling of peptides demonstrated an apparent co-localization of PP and FMRFamide immunoreactivities in the same dense-cored vesicles, although populations of dense-cored vesicles that labelled solely for FMRFamide were also evident. Antigen preabsorption studies indicated little or no cross-reactivity between the two antisera.

Subcellular localization of GABA receptors in the central nervous system using post-embedding immunohistochemistry

2002 ◽  
Vol 9 (3) ◽  
pp. 173-180 ◽  
Author(s):  
Wendy L Heck ◽  
Arkadiusz Slusarczyk ◽  
Ana Maria Basaraba ◽  
Laura Schweitzer
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Subcellular Localization ◽  
Gaba Receptors ◽  
The Central Nervous System
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