The product of Saccharomyces cerevisiae WHIP/MGS1, a gene related to replication factor C genes, interacts functionally with DNA polymerase δ

2002 ◽  
Vol 268 (3) ◽  
pp. 371-386 ◽  
Author(s):  
D. Branzei ◽  
M. Seki ◽  
F. Onoda ◽  
T. Enomoto
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Dna Polymerase ◽  
Replication Factor C ◽  
Replication Factor ◽  
Dna Polymerase Δ ◽  
Factor C

Functional sites of human PCNA which interact with p21 (Cip1/Waf1), DNA polymerase δ and replication factor C

1998 ◽  
Vol 3 (6) ◽  
pp. 357-369 ◽  
Author(s):  
Takashi Oku ◽  
Soichiro Ikeda ◽  
Hisashi Sasaki ◽  
Kotaro Fukuda ◽  
Hiroshi Morioka ◽  
...  
Keyword(s):  
Dna Polymerase ◽  
Replication Factor C ◽  
Functional Sites ◽  
Replication Factor ◽  
Dna Polymerase Δ ◽  
Factor C

scholarly journals Specific DNA replication mutations affect telomere length in Saccharomyces cerevisiae.

1996 ◽  
Vol 16 (9) ◽  
pp. 4614-4620 ◽  
Author(s):  
A K Adams ◽  
C Holm
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Dna Replication ◽  
Telomere Length ◽  
Dna Polymerase ◽  
Structural Gene ◽  
Large Subunit ◽  
Replication Factor C ◽  
Telomere Elongation ◽  
Replication Factor ◽  
Factor C

To investigate the relationship between the DNA replication apparatus and the control of telomere length, we examined the effects of several DNA replication mutations on telomere length in Saccharomyces cerevisiae. We report that a mutation in the structural gene for the large subunit of DNA replication factor C (cdc44/rfc1) causes striking increases in telomere length. A similar effect is seen with mutations in only one other DNA replication gene: the structural gene for DNA polymerase alpha (cdc17/pol1) (M.J. Carson and L. Hartwell, Cell 42:249-257, 1985). For both genes, the telomere elongation phenotype is allele specific and appears to correlate with the penetrance of the mutations. Furthermore, fluorescence-activated cell sorter analysis reveals that those alleles that cause elongation also exhibit a slowing of DNA replication. To determine whether elongation is mediated by telomerase or by slippage of the DNA polymerase, we created cdc17-1 mutants carrying deletions of the gene encoding the RNA component of telomerase (TLC1). cdc17-1 strains that would normally undergo telomere elongation failed to do so in the absence of telomerase activity. This result implies that telomere elongation in cdc17-1 mutants is mediated by the action of telomerase. Since DNA replication involves transfer of the nascent strand from polymerase alpha to replication factor C (T. Tsurimoto and B. Stillman, J. Biol. Chem. 266:1950-1960, 1991; T. Tsurimoto and B. Stillman, J. Biol. Chem. 266:1961-1968, 1991; S. Waga and B. Stillman, Nature [London] 369:207-212, 1994), one possibility is that this step affects the regulation of telomere length.

scholarly journals The Large Subunit of Replication Factor C (Rfclp/Cdc44p) Is Required for DNA Replication and DNA Repair in Saccharomyces cerevisiae

Genetics ◽  
1996 ◽  
Vol 142 (1) ◽  
pp. 65-78 ◽  
Author(s):  
Michael A McAlear ◽  
K Michelle Tuffo ◽  
Connie Holm
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Dna Repair ◽  
Dna Replication ◽  
Uv Radiation ◽  
Large Subunit ◽  
Restrictive Temperature ◽  
Replication Factor C ◽  
Replication Factor ◽  
Factor C

We used genetic and biochemical techniques to characterize the phenotypes associated with mutations affecting the large subunit of replication factor C (Cdc44p or Rfc1p) in Saccharomyces cerevisiae. We demonstrate that Cdc44p is required for both DNA replication and DNA repair in vivo. Cold-sensitive cdc44 mutants experience a delay in traversing S phase at the restrictive temperature following alpha factor arrest; although mutant cells eventually accumulate with a G2/M DNA content, they undergo a cell cycle arrest and initiate neither mitosis nor a new round of DNA synthesis. cdc44 mutants also exhibit an elevated level of spontaneous mutation, and they are sensitive both to the DNA damaging agent methylmethane sulfonate and to exposure to UV radiation. After exposure to UV radiation, cdc44 mutants at the restrictive temperature contain higher levels of single-stranded DNA breaks than do wild-type cells. This observation is consistent with the hypothesis that Cdc44p is involved in repairing gaps in the DNA after the excision of damaged bases. Thus, Cdc44p plays an important role in both DNA replication and DNA repair in vivo.

Identification of replication factor C from Saccharomyces cerevisiae: a component of the leading-strand DNA replication complex

1992 ◽  
Vol 12 (1) ◽  
pp. 155-163 ◽  
Author(s):  
K Fien ◽  
B Stillman
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Dna Replication ◽  
Dna Polymerase ◽  
Replication Factor C ◽  
Dna Polymerase Delta ◽  
Replication Complex ◽  
Leading Strand ◽  
Factor C ◽  
Sv40 Dna

A number of proteins have been isolated from human cells on the basis of their ability to support DNA replication in vitro of the simian virus 40 (SV40) origin of DNA replication. One such protein, replication factor C (RFC), functions with the proliferating cell nuclear antigen (PCNA), replication protein A (RPA), and DNA polymerase delta to synthesize the leading strand at a replication fork. To determine whether these proteins perform similar roles during replication of DNA from origins in cellular chromosomes, we have begun to characterize functionally homologous proteins from the yeast Saccharomyces cerevisiae. RFC from S. cerevisiae was purified by its ability to stimulate yeast DNA polymerase delta on a primed single-stranded DNA template in the presence of yeast PCNA and RPA. Like its human-cell counterpart, RFC from S. cerevisiae (scRFC) has an associated DNA-activated ATPase activity as well as a primer-template, structure-specific DNA binding activity. By analogy with the phage T4 and SV40 DNA replication in vitro systems, the yeast RFC, PCNA, RPA, and DNA polymerase delta activities function together as a leading-strand DNA replication complex. Now that RFC from S. cerevisiae has been purified, all seven cellular factors previously shown to be required for SV40 DNA replication in vitro have been identified in S. cerevisiae.

scholarly journals Physical Links Between the Nuclear Envelope Protein Mps3, Three Alternate Replication Factor C Complexes, and a Variant Histone in Saccharomyces cerevisiae

2012 ◽  
Vol 31 (6) ◽  
pp. 917-924 ◽  
Author(s):  
Jared Haas ◽  
Amanda Lemoncelli ◽  
Christina Morozov ◽  
Karl Franke ◽  
John Dominder ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Nuclear Envelope ◽  
Envelope Protein ◽  
Replication Factor C ◽  
Replication Factor ◽  
Factor C

DNA polymerase switching: II. Replication factor C abrogates primer synthesis by DNA polymerase α at a critical length

2000 ◽  
Vol 295 (4) ◽  
pp. 803-814 ◽  
Author(s):  
Romina Mossi ◽  
Robert C Keller ◽  
Elena Ferrari ◽  
Ulrich Hübscher
Keyword(s):  
Dna Polymerase ◽  
Critical Length ◽  
Replication Factor C ◽  
Replication Factor ◽  
Dna Polymerase Α ◽  
Factor C
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