Influence of apical fluid volume on the development of functional intercellular junctions in the human epithelial cell line 16HBE14o- : implications for the use of this cell line as an in vitro model for bronchial drug absorption studies

2002 ◽  
Vol 308 (3) ◽  
pp. 391-400 ◽  
Author(s):  
Carsten Ehrhardt ◽  
Carsten Kneuer ◽  
Jennifer Fiegel ◽  
Justin Hanes ◽  
Ulrich Schaefer ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Cell Line ◽  
Drug Absorption ◽  
In Vitro Model ◽  
Intercellular Junctions ◽  
Epithelial Cell Line ◽  
Human Epithelial Cell ◽  
Absorption Studies ◽  
Vitro Model

Establishment and Characterization of an SV40 Large T Antigen-Transduced Porcine Colonic Epithelial Cell Line

2017 ◽  
Vol 203 (5) ◽  
pp. 267-286 ◽  
Author(s):  
Bastian Kaiser ◽  
Martina Böttner ◽  
Thilo Wedel ◽  
Ronald M. Brunner ◽  
Tom Goldammer ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Cell Line ◽  
In Vitro Model ◽  
T Antigen ◽  
Epithelial Barrier ◽  
Epithelial Cell Line ◽  
Large T Antigen ◽  
Epithelial Barrier Function ◽  
Vitro Model

Continuous cell lines have become indispensable tools that have enabled investigations into cellular mechanisms by increasing experimental reproducibility and sample availability, and decreasing the use of experimental animals. To facilitate studies of epithelial barrier function of the porcine colon, we aimed to establish an epithelial cell line with an extended replicative capacity. Cells were isolated from the proximal colon of a 3-week-old piglet and transduced using a recombinant retroviral vector construct containing the simian virus 40 large T antigen (SV40 TAg). We established a clonal epithelial cell line, referred to as PoCo83-3, that stably expressed the SV40 TAg, verified at mRNA and protein levels. PoCo83-3 showed epithelial cell-specific features, such as cobblestone-like morphology, dome structure formation, the presence of apical microvilli, and the expression of keratin 18, E-cadherin and the tight junction-associated proteins zonula occludens-1, occludin, and claudin-1. To validate PoCo83-3 as an in vitro model in epithelial barrier research, proinflammatory cytokine-inducible alterations in barrier integrity were demonstrated by incubating the cells with TNF-α and IFN-γ for 48 h. These cytokine treatments promoted a decreased transepithelial electrical resistance. In summary, PoCo83-3 exhibited an extended life span and a differentiated phenotype while maintaining epithelial characteristics. Based on these results, we present this cell line as a valuable in vitro model for investigations of epithelial barrier function in the porcine colon.

scholarly journals A 3D “In Vitro” Model to Study Hyaluronan Effect in Nasal Epithelial Cell Line Exposed to Double-Stranded RNA Poly(I:C)

2020 ◽  
Vol 28 (3) ◽  
pp. 272-281
Author(s):  
Giusy Daniela Albano ◽  
Anna Bonanno ◽  
Daniela Giacomazza ◽  
Luca Cavalieri ◽  
Martina Sammarco ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Cell Line ◽  
In Vitro Model ◽  
Poly I:C ◽  
Epithelial Cell Line ◽  
Nasal Epithelial Cell ◽  
Double Stranded Rna ◽  
3D In Vitro Model ◽  
Vitro Model

Monolayers of porcine alveolar epithelial cells in primary culture as an in vitro model for drug absorption studies

2007 ◽  
Vol 66 (3) ◽  
pp. 372-382 ◽  
Author(s):  
Anne Steimer ◽  
Helmut Franke ◽  
Eleonore Haltner-Ukomado ◽  
Michael Laue ◽  
Carsten Ehrhardt ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Primary Culture ◽  
Drug Absorption ◽  
In Vitro Model ◽  
Alveolar Epithelial Cells ◽  
Alveolar Epithelial ◽  
Absorption Studies ◽  
Vitro Model
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