Oxygen-limited fed-batch process: an alternative control for Pichia pastoris recombinant protein processes

2005 ◽  
Vol 27 (6) ◽  
pp. 399-406 ◽  
Author(s):  
Theppanya Charoenrat ◽  
Mariena Ketudat-Cairns ◽  
Helle Stendahl-Andersen ◽  
Mehmedalija Jahic ◽  
Sven-Olof Enfors
Keyword(s):  
Pichia Pastoris ◽  
Recombinant Protein ◽  
Batch Process ◽  
Fed Batch ◽  
Alternative Control

scholarly journals Influence of trace elements supplementation on the production of recombinant frutalin by Pichia pastoris KM71H in fed-batch process

2013 ◽  
Vol 67 (7) ◽  
Author(s):  
Marcela Wanderley ◽  
Carla Oliveira ◽  
Danyelly Bruneska ◽  
Lucília Domingues ◽  
José Lima Filho ◽  
...  
Keyword(s):  
Trace Elements ◽  
Pichia Pastoris ◽  
Recombinant Protein ◽  
Protein Production ◽  
Batch Process ◽  
Fermentation Medium ◽  
Fed Batch ◽  
Stirred Tank Bioreactor ◽  
Recombinant Pichia Pastoris ◽  
Fed Batch Fermentation

AbstractFrutalin, a galactose-specific lectin used to detect specific tumour markers, is a protein with low expression level in breadfruit. In the present study, fed-batch fermentation in a stirred tank bioreactor was used as a strategy to enhance protein production by a recombinant Pichia pastoris KM71H. By using this process, the production of recombinant frutalin was 4-fold higher than the value obtained in shaker flasks batch assays. Supplementation of the fermentation medium with trace elements (Pichia trace minerals, PTM) was also evaluated in order to stimulate production of the recombinant protein. The addition of PTM to the minimum medium afforded a recombinant protein production of 13.4 mg L−1, which was 2.5-fold higher than that achieved from the culture medium without PTM supplementation. These results are significant as the development of strategies to improve the production of recombinant frutalin may broaden its application in cancer diagnosis.

Estimation of Bioreactor Efficiency Through Structured Hydrodynamic Modeling Case Study of a Pichia pastoris Fed-Batch Process

2005 ◽  
pp. 653-671 ◽  
Author(s):  
Frank Delvigne ◽  
Thami El Mejdoub ◽  
Jacqueline Destain ◽  
Jean-Marc Delroisse ◽  
Micheline Vandenbol ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Batch Process ◽  
Hydrodynamic Modeling ◽  
Fed Batch

scholarly journals Biomass soft sensor for a Pichia pastoris fed‐batch process based on phase detection and hybrid modeling

2020 ◽  
Vol 117 (9) ◽  
pp. 2749-2759
Author(s):  
Vincent Brunner ◽  
Manuel Siegl ◽  
Dominik Geier ◽  
Thomas Becker
Keyword(s):  
Pichia Pastoris ◽  
Batch Process ◽  
Soft Sensor ◽  
Hybrid Modeling ◽  
Phase Detection ◽  
Fed Batch

Estimation of Bioreactor Efficiency Through Structured Hydrodynamic Modeling Case Study of a Pichia pastoris Fed-Batch Process

2005 ◽  
Vol 122 (1-3) ◽  
pp. 0653-0672 ◽  
Author(s):  
Frank Delvigne ◽  
Thami El Mejdoub ◽  
Jacqueline Destain ◽  
Jean-Marc Delroisse ◽  
Micheline Vandenbol ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Batch Process ◽  
Hydrodynamic Modeling ◽  
Fed Batch

scholarly journals Development of a fed-batch process for a recombinant Pichia pastoris Δoch1 strain expressing a plant peroxidase

2015 ◽  
Vol 14 (1) ◽  
Author(s):  
Christoph Gmeiner ◽  
Amirhossein Saadati ◽  
Daniel Maresch ◽  
Stanimira Krasteva ◽  
Manuela Frank ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Batch Process ◽  
Fed Batch ◽  
Plant Peroxidase ◽  
Recombinant Pichia Pastoris

scholarly journals Bioprocess performance analysis of novel methanol-independent promoters for recombinant protein production with Pichia pastoris

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Javier Garrigós-Martínez ◽  
Kiira Vuoristo ◽  
Miguel Angel Nieto-Taype ◽  
Juha Tähtiharju ◽  
Jaana Uusitalo ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Recombinant Protein ◽  
Recombinant Protein Production ◽  
Protein Production ◽  
Expression System ◽  
Cell Factory ◽  
The Novel ◽  
Expression Systems ◽  
Fed Batch ◽  
Model Protein

Abstract Background Pichia pastoris is a powerful and broadly used host for recombinant protein production (RPP), where past bioprocess performance has often been directed with the methanol regulated AOX1 promoter (PAOX1), and the constitutive GAP promoter (PGAP). Since promoters play a crucial role in an expression system and the bioprocess efficiency, innovative alternatives are constantly developed and implemented. Here, a thorough comparative kinetic characterization of two expression systems based on the commercial PDF and UPP promoters (PPDF, PUPP) was first conducted in chemostat cultures. Most promising conditions were subsequently tested in fed-batch cultivations. These new alternatives were compared with the classical strong promoter PGAP, using the Candida antarctica lipase B (CalB) as model protein for expression system performance. Results Both the PPDF and PUPP-based expression systems outperformed similar PGAP-based expression in chemostat cultivations, reaching ninefold higher specific production rates (qp). CALB transcription levels were drastically higher when employing the novel expression systems. This higher expression was also correlated with a marked upregulation of unfolded protein response (UPR) related genes, likely from an increased protein burden in the endoplasmic reticulum (ER). Based on the chemostat results obtained, best culture strategies for both PPDF and PUPP expression systems were also successfully implemented in 15 L fed-batch cultivations where qp and product to biomass yield (YP/X*) values were similar than those obtained in chemostat cultivations. Conclusions As an outcome of the macrokinetic characterization presented, the novel PPDF and PUPP were observed to offer much higher efficiency for CalB production than the widely used PGAP-based methanol-free alternative. Thus, both systems arise as highly productive alternatives for P. pastoris-based RPP bioprocesses. Furthermore, the different expression regulation patterns observed indicate the level of gene expression can be adjusted, or tuned, which is interesting when using Pichia pastoris as a cell factory for different products of interest.

Development of a Fed-Batch Process for the Production of a Recombinant Protein X in CHO-GS System – Case Study from the Cell to Reactor Process Ready for Pilot Scale Cultivation

2010 ◽  
pp. 723-725
Author(s):  
Véronique Chotteau ◽  
Yun Jiang ◽  
Jeannette Westin ◽  
Kerstin Dahlenborg ◽  
Anna Sjöblom-Hallén ◽  
...  
Keyword(s):  
Recombinant Protein ◽  
Batch Process ◽  
Pilot Scale ◽  
Fed Batch ◽  
Protein X

Development of an automated fed batch process for Pichia pastoris with biomass and methanol sensor technology supported by simulation

2014 ◽  
Vol 185 ◽  
pp. S57-S58
Author(s):  
Hagen Müller ◽  
Jennifer Kuhn ◽  
Igor Stempin ◽  
Denise Salzig ◽  
Peter Czermak
Keyword(s):  
Pichia Pastoris ◽  
Batch Process ◽  
Sensor Technology ◽  
Fed Batch ◽  
Methanol Sensor
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