scholarly journals Biomass soft sensor for a Pichia pastoris fed‐batch process based on phase detection and hybrid modeling

2020 ◽  
Vol 117 (9) ◽  
pp. 2749-2759
Author(s):  
Vincent Brunner ◽  
Manuel Siegl ◽  
Dominik Geier ◽  
Thomas Becker
Keyword(s):  
Pichia Pastoris ◽  
Batch Process ◽  
Soft Sensor ◽  
Hybrid Modeling ◽  
Phase Detection ◽  
Fed Batch

Estimation of Bioreactor Efficiency Through Structured Hydrodynamic Modeling Case Study of a Pichia pastoris Fed-Batch Process

2005 ◽  
pp. 653-671 ◽  
Author(s):  
Frank Delvigne ◽  
Thami El Mejdoub ◽  
Jacqueline Destain ◽  
Jean-Marc Delroisse ◽  
Micheline Vandenbol ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Batch Process ◽  
Hydrodynamic Modeling ◽  
Fed Batch

Estimation of Bioreactor Efficiency Through Structured Hydrodynamic Modeling Case Study of a Pichia pastoris Fed-Batch Process

2005 ◽  
Vol 122 (1-3) ◽  
pp. 0653-0672 ◽  
Author(s):  
Frank Delvigne ◽  
Thami El Mejdoub ◽  
Jacqueline Destain ◽  
Jean-Marc Delroisse ◽  
Micheline Vandenbol ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Batch Process ◽  
Hydrodynamic Modeling ◽  
Fed Batch

scholarly journals Development of a fed-batch process for a recombinant Pichia pastoris Δoch1 strain expressing a plant peroxidase

2015 ◽  
Vol 14 (1) ◽  
Author(s):  
Christoph Gmeiner ◽  
Amirhossein Saadati ◽  
Daniel Maresch ◽  
Stanimira Krasteva ◽  
Manuela Frank ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Batch Process ◽  
Fed Batch ◽  
Plant Peroxidase ◽  
Recombinant Pichia Pastoris

scholarly journals Influence of trace elements supplementation on the production of recombinant frutalin by Pichia pastoris KM71H in fed-batch process

2013 ◽  
Vol 67 (7) ◽  
Author(s):  
Marcela Wanderley ◽  
Carla Oliveira ◽  
Danyelly Bruneska ◽  
Lucília Domingues ◽  
José Lima Filho ◽  
...  
Keyword(s):  
Trace Elements ◽  
Pichia Pastoris ◽  
Recombinant Protein ◽  
Protein Production ◽  
Batch Process ◽  
Fermentation Medium ◽  
Fed Batch ◽  
Stirred Tank Bioreactor ◽  
Recombinant Pichia Pastoris ◽  
Fed Batch Fermentation

AbstractFrutalin, a galactose-specific lectin used to detect specific tumour markers, is a protein with low expression level in breadfruit. In the present study, fed-batch fermentation in a stirred tank bioreactor was used as a strategy to enhance protein production by a recombinant Pichia pastoris KM71H. By using this process, the production of recombinant frutalin was 4-fold higher than the value obtained in shaker flasks batch assays. Supplementation of the fermentation medium with trace elements (Pichia trace minerals, PTM) was also evaluated in order to stimulate production of the recombinant protein. The addition of PTM to the minimum medium afforded a recombinant protein production of 13.4 mg L−1, which was 2.5-fold higher than that achieved from the culture medium without PTM supplementation. These results are significant as the development of strategies to improve the production of recombinant frutalin may broaden its application in cancer diagnosis.

Development of an automated fed batch process for Pichia pastoris with biomass and methanol sensor technology supported by simulation

2014 ◽  
Vol 185 ◽  
pp. S57-S58
Author(s):  
Hagen Müller ◽  
Jennifer Kuhn ◽  
Igor Stempin ◽  
Denise Salzig ◽  
Peter Czermak
Keyword(s):  
Pichia Pastoris ◽  
Batch Process ◽  
Sensor Technology ◽  
Fed Batch ◽  
Methanol Sensor

Oxygen-limited fed-batch process: an alternative control for Pichia pastoris recombinant protein processes

2005 ◽  
Vol 27 (6) ◽  
pp. 399-406 ◽  
Author(s):  
Theppanya Charoenrat ◽  
Mariena Ketudat-Cairns ◽  
Helle Stendahl-Andersen ◽  
Mehmedalija Jahic ◽  
Sven-Olof Enfors
Keyword(s):  
Pichia Pastoris ◽  
Recombinant Protein ◽  
Batch Process ◽  
Fed Batch ◽  
Alternative Control

High protein expression in fermentation of recombinant Pichia pastoris by a fed-batch process

1997 ◽  
Vol 32 (2) ◽  
pp. 107-111 ◽  
Author(s):  
Yinliang Chen ◽  
Julia Cino ◽  
Glen Hart ◽  
David Freedman ◽  
Christopher White ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Protein Expression ◽  
Batch Process ◽  
High Protein ◽  
Fed Batch ◽  
Recombinant Pichia Pastoris

Production of Rhamnolipid Biosurfactant from Fed Batch Culture by Pseudomonas aeruginosa using Multiple Substrates

2020 ◽  
Vol 16 (6) ◽  
pp. 928-933
Author(s):  
Jujjavarapu S. Eswari
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Batch Process ◽  
Time Interval ◽  
Fed Batch ◽  
Multiple Substrates ◽  
Surface Active Agents ◽  
Limiting Nutrients ◽  
Limiting Nutrient ◽  
Surface Active ◽  
Rhamnolipid Biosurfactant

Objective: Biosurfactants are the surface active agents which are used for the reduction of surface and interfacial tensions of liquids. Rhamnolipids are the surfactants produced by Pseudomonas aeruginosa. It requires minimum nutrition for its growth as it can also grow in distilled water. The rhamnolipids produced by Pseudomonas aeruginosa are extra-cellular glycolipids consisting of L-rhamnose and 3-hydroxyalkanoic acid. Methods: The fed-batch method for the rhamnolipid production is considered in this study to know the influence of the carbon, nitrogen, phosphorous substrates as growth-limiting nutrients. Pulse feeding is employed for limiting nutrient addition at particular time interval to obtain maximum rhamnolipid formation from Pseudomonas aeruginosa compared with the batch process. Results: Out of 3 fed batch strategies constant glucose fed batch strategy shows best and gave maximum rhamnolipid concentration of 0.134 g/l.

scholarly journals Establishing a Fed‐Batch Process for Protease Expression with Bacillus licheniformis in Polymer‐Based Controlled‐Release Microtiter Plates

2019 ◽  
Vol 15 (2) ◽  
pp. 1900088 ◽  
Author(s):  
Tobias Habicher ◽  
Edward K. A. Rauls ◽  
Franziska Egidi ◽  
Timm Keil ◽  
Tobias Klein ◽  
...  
Keyword(s):  
Controlled Release ◽  
Bacillus Licheniformis ◽  
Batch Process ◽  
Fed Batch ◽  
Microtiter Plates ◽  
Protease Expression
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