Alginate immobilization of recombinant Escherichia coli whole cells harboring l-arabinose isomerase for l-ribulose production

2009 ◽  
Vol 33 (6) ◽  
pp. 741-748 ◽  
Author(s):  
Ye-Wang Zhang ◽  
Ponnandy Prabhu ◽  
Jung-Kul Lee
Keyword(s):  
Escherichia Coli ◽  
Recombinant Escherichia Coli ◽  
Whole Cells ◽  
Alginate Immobilization ◽  
Arabinose Isomerase

scholarly journals Differential Selectivity of the Escherichia coli Cell Membrane Shifts the Equilibrium for the Enzyme-Catalyzed Isomerization of Galactose to Tagatose

2008 ◽  
Vol 74 (8) ◽  
pp. 2307-2313 ◽  
Author(s):  
Jin-Ha Kim ◽  
Byung-Chul Lim ◽  
Soo-Jin Yeom ◽  
Yeong-Su Kim ◽  
Hye-Jung Kim ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Cell Membrane ◽  
Gene Knockout ◽  
Equilibrium Constants ◽  
Isomerization Reaction ◽  
Whole Cells ◽  
Cell Conversion ◽  
Equilibrium Shift ◽  
Differential Selectivity ◽  
Arabinose Isomerase

ABSTRACT An Escherichia coli galactose kinase gene knockout (ΔgalK) strain, which contains the l-arabinose isomerase gene (araA) to isomerize d-galactose to d-tagatose, showed a high conversion yield of tagatose compared with the original galK strain because galactose was not metabolized by endogenous galactose kinase. In whole cells of the ΔgalK strain, the isomerase-catalyzed reaction exhibited an equilibrium shift toward tagatose, producing a tagatose fraction of 68% at 37°C, whereas the purified l-arabinose isomerase gave a tagatose equilibrium fraction of 36%. These equilibrium fractions are close to those predicted from the measured equilibrium constants of the isomerization reaction catalyzed in whole cells and by the purified enzyme. The equilibrium shift in these cells resulted from the higher uptake and lower release rates for galactose, which is a common sugar substrate, than for tagatose, which is a rare sugar product. A ΔmglB mutant had decreased uptake rates for galactose and tagatose, indicating that a methylgalactoside transport system, MglABC, is the primary contributing transporter for the sugars. In the present study, whole-cell conversion using differential selectivity of the cell membrane was proposed as a method for shifting the equilibrium in sugar isomerization reactions.

Production of tropane alkaloids by biotransformation using recombinant Escherichia coli whole cells

2017 ◽  
Vol 125 ◽  
pp. 180-189 ◽  
Author(s):  
Alejandra B. Cardillo ◽  
María Perassolo ◽  
Mariela Sartuqui ◽  
Julián Rodríguez Talou ◽  
Ana M. Giulietti
Keyword(s):  
Escherichia Coli ◽  
Tropane Alkaloids ◽  
Recombinant Escherichia Coli ◽  
Whole Cells

scholarly journals Biocatalytic Production of Trehalose from Maltose by Using Whole Cells of Permeabilized Recombinant Escherichia coli

PLoS ONE ◽  
2015 ◽  
Vol 10 (10) ◽  
pp. e0140477 ◽  
Author(s):  
Zhaojuan Zheng ◽  
Ying Xu ◽  
Ye Sun ◽  
Wending Mei ◽  
Jia Ouyang
Keyword(s):  
Escherichia Coli ◽  
Recombinant Escherichia Coli ◽  
Whole Cells ◽  
Biocatalytic Production
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