Shoot proliferation and in vitro organogenesis from shoot apex and cotyledonary explants of royal poinciana (Delonix regia), an ornamental leguminous tree

Trees ◽  
2019 ◽  
Vol 34 (1) ◽  
pp. 189-197 ◽  
Author(s):  
Andrey de Oliveira Costa ◽  
Lázara Aline Simões Silva ◽  
Isabella Mendes Duarte ◽  
Mariana Machado ◽  
Givanildo Zildo da Silva ◽  
...  
Keyword(s):  
Shoot Apex ◽  
Shoot Proliferation ◽  
Leguminous Tree ◽  
In Vitro Organogenesis ◽  
Delonix Regia ◽  
Cotyledonary Explants

scholarly journals The Effects of Growth Regulators on Shoot Propagation and Rooting of Vitis Following in Vitro Axillary Bud and Shoot Apex Culture

HortScience ◽  
1997 ◽  
Vol 32 (3) ◽  
pp. 516C-516
Author(s):  
Handan Büyükdemirci ◽  
Paul E. Read
Keyword(s):  
Shoot Apex ◽  
Shoot Growth ◽  
Shoot Proliferation ◽  
Multiple Shoots ◽  
Multiple Shoot ◽  
Axillary Buds ◽  
Axillary Bud ◽  
Ms Medium ◽  
Axillary Bud Culture

Axillary buds of `Valiant' grapevine (Vitis spp.) grown in vitro were transferred onto Murashige and Skoog (MS) medium supplemented with different cytokinin and auxin combinations and concentrations. It was found that culture medium caused statistically important differences in number of nodes, number of fully expanded leaves, number of multiple shoots, number of roots, and length of shoots. MS medium supplemented with 1.0 mg BA/liter in combination with 0.01 mg NAA/L was found to be the best medium for shoot growth and callus production. MS medium supplemented with the combination of 0.5 mg BA/L and 0.01 mg NAA/L was the best medium for explant rooting. The medium containing BA and NAA encouraged better shoot growth than those containing BA alone. When the concentration of BA in the medium was increased, multiple shoot proliferation and teratological structures of explants increased, but the number of small leaves and length of internode decreased. Axillary bud culture led to better shoot growth than was found for shoot apex culture. The presence of leaves positively affected shoot growth from axillary buds. Also placing the axillary buds horizontally onto the medium gave better shoot proliferation and growth than placing them vertically.

Development of an Agrobacterium-mediated Transformation System for `Beurre Bosc' Pear

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 461d-461
Author(s):  
Richard L. Bell ◽  
Ralph Scorza ◽  
Chinnathambi Srinivasan
Keyword(s):  
Shoot Proliferation ◽  
Induction Medium ◽  
Shoot Induction ◽  
Transformation System ◽  
Gus Histochemical Assay ◽  
Young Leaves ◽  
Leaf Tissues ◽  
Shoot Induction Medium ◽  
Basal Salts

An efficient regeneration/transformation system was developed for `Beurre Bosc' pear. Young leaves were harvested from in vitro shoots proliferated on a medium containing MS basal salts and 5 BAP, 0.5 μM IBA, and 0.6M3. Shoot regeneration was optimized using a modification of the medium of Chevreau and Leblay (1993). Explants were cultured on shoot induction medium contained 10 μM TDZ and 1 μM IBA for 4 weeks in the dark, and then transfered to a similar, but auxinless, regeneration medium until shoots developed, usually after an additional 4 to 8 weeks. Leaf tissues were transformed by co-cultivation for 3 days with Agrobacterium tumefaciens EHA101 carrying a pGA482 plasmid containing NPTII, GUS, and rolC genes, followed by cultivation on SIM containing 300 mg/L timentin. Putative transgenic plants were selected on shoot induction medium containing 80mg/L kanamycin, and multiplied on shoot proliferation medium. Four clones were confirmed as transgenic using the GUS histochemical assay and Southern blots for the NPTII and rolC genes. Plants of each clone have been rooted and successfully transfered to the greenhouse for further analysis of gene expression.

scholarly journals Biochemical profile of cuttings used for in vitro organogenesis of Prunus africana: An endangered species in Cameroon

2015 ◽  
Vol 14 (33) ◽  
pp. 2568-2575
Author(s):  
Germo Nzweundji Justine ◽  
Niemenak Nicolas ◽  
Oumar ◽  
Judith Tsafack Julie ◽  
Konan Koffi ◽  
...  
Keyword(s):  
Endangered Species ◽  
Biochemical Profile ◽  
In Vitro Organogenesis ◽  
Prunus Africana

In vitro shoot apex micrografting of mature Acacia mangium

1996 ◽  
Vol 34 (2) ◽  
pp. 213-217 ◽  
Author(s):  
O. Monteuuis
Keyword(s):  
Shoot Apex ◽  
Acacia Mangium

scholarly journals An efficient protocol for in vitro organogenesis and antioxidant studies in Melia dubia Cav.

2016 ◽  
Vol 15 (19) ◽  
pp. 768-775 ◽  
Author(s):  
Naseer Shah Syed ◽  
Ahmad Wani Tareq ◽  
Ram Bhimi ◽  
Koul Monika ◽  
Awasthi Praveen ◽  
...  
Keyword(s):  
In Vitro Organogenesis ◽  
Melia Dubia

scholarly journals In Vitro Micropropagation of the Medicinal Plant Physalis angulata L.

2016 ◽  
Vol 8 (2) ◽  
pp. 161-163
Author(s):  
Owk ANIEL KUMAR ◽  
Songa RAMESH ◽  
Sape SUBBA TATA
Keyword(s):  
Large Scale ◽  
Leaf Disc ◽  
Basal Medium ◽  
Shoot Proliferation ◽  
Shoot Multiplication ◽  
Medicinal Herb ◽  
Root Induction ◽  
Survival Percentage ◽  
Physalis Angulata

Physalis angulata L. is an important medicinal herb. An efficient direct adventitious plant regeneration protocol was developed for large scale propagation using leaf disc as explants. The explants were cultured on MS basal medium supplemented with 0.25-3.0 mg/L 6-benzyl amino purine (BAP) for primary shoot proliferation. Inclusion of indole-3-acetic acid (IAA) and gibberellic acid (GA3) in the culture medium along with BAP promoted a higher rate of shoot multiplication. The maximum number of shoots was produced in MS + BAP (1.0 mg/L) + IAA (0.5 mg/L) + GA3 (0.20 mg/L) after the third subculture. An average of 152.8 ± 0.40 shoots were produced from each leaf disc. For root induction the shootlets were transferred to MS medium supplemented with different concentrations of indole-3-butyric acid (IBA). The highest percentage of root induction was observed in 1.0 mg/L (IBA). Rooted plants were successfully established in the soil after hardening. The survival percentage of rooted plants on soil was found to be 85%. This result will facilitate the conservation and propagation of the important medicinal herb Physalis angulata L.

scholarly journals Effect of Media Types and Carbon Sources on In vitro Shoot Proliferation of Spilanthes acmella (L.) Murray

2014 ◽  
Vol 23 (2) ◽  
pp. 283-288
Author(s):  
Veenu Joshi ◽  
S. K. Jadhav
Keyword(s):  
Carbon Sources ◽  
Shoot Proliferation ◽  
Spilanthes Acmella ◽  
Media Types ◽  
In Vitro Shoot Proliferation

D. O. I. http://dx.doi.org/10.3329/ptcb.v23i2.17529Plant Tissue Cult. & Biotech. 23(2): 283-288, 2013  (December)

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