In vitro shoot apex micrografting of mature Acacia mangium

1996 ◽  
Vol 34 (2) ◽  
pp. 213-217 ◽  
Author(s):  
O. Monteuuis
Keyword(s):  
2007 ◽  
Vol 42 (4) ◽  
pp. 573-579 ◽  
Author(s):  
Domingos Sávio Campos Paciullo ◽  
Carlos Augusto Brandão de Carvalho ◽  
Luiz Januário Magalhães Aroeira ◽  
Mirton José Frota Morenz ◽  
Fernando César Ferraz Lopes ◽  
...  

O objetivo deste trabalho foi avaliar características morfofisiológicas e o valor nutritivo da Brachiaria decumbens, nas épocas chuvosas de 2003 e 2004, em um sistema silvipastoril, com faixas de 30 m de largura com essa gramínea, alternadas com faixas de 10 m com árvores de Acacia mangium, A. angustissima, Mimosa artemisiana, Leucaena leucocephala x L. diversifolia, Eucalyptus grandis. Foi adotado o delineamento de blocos ao acaso, em parcelas subsubdivididas (características morfofisiológicas) ou parcelas subdivididas (características do valor nutritivo), com quatro repetições. Em 2003, o sombreamento foi de 65% da radiação incidente fora do bosque e, em 2004, de 35%, depois da retirada de árvores de eucalipto. Sob sombreamento, a densidade de perfilhos, o índice de área foliar e a massa de forragem foram maiores no segundo ano. Os teores médios de fibra em detergente neutro (FDN) foram maiores a pleno sol que sob sombreamento, enquanto os valores médios de proteína bruta (PB) e a digestibilidade in vitro da matéria seca foram maiores à sombra. O sombreamento moderado aumenta os teores de PB, reduz os teores de FDN e incrementa a digestibilidade da forragem de gramíneas que crescem sob a copa das árvores.


1980 ◽  
Vol 49 (2) ◽  
pp. 193-197 ◽  
Author(s):  
YASUNORI KODA ◽  
YOZO OKAZAWA
Keyword(s):  

2009 ◽  
Vol 75 (24) ◽  
pp. 7752-7759 ◽  
Author(s):  
Christine Le Roux ◽  
Diana Tentchev ◽  
Yves Prin ◽  
Doreen Goh ◽  
Yani Japarudin ◽  
...  

ABSTRACT In the context of an increasing utilization of the interspecific hybrid Acacia mangium × A. auriculiformis as a plantation tree in the tropical humid zone, its symbiotic characterization was carried out in comparison with that of its two parental species. Rhizobium strains of diverse geographical origins were isolated from root nodules of the hybrid and its parents. Almost all Acacia hybrid isolates were fast growing on yeast extract-mannitol medium, in contrast to those isolated from both parental species, which were mostly slow growing. The rhizobium strains were characterized through partial sequencing of the rRNA operon. In the phylogenetic tree, almost all strains isolated from the hybrid were grouped together in a clade close to Bradyrhizobium japonicum, while all strains isolated from both parental species were close to Bradyrhizobium elkanii. Inoculation experiments performed under in vitro or greenhouse conditions showed that all strains were infective with their original hosts but exhibited very variable degrees of effectivity according to the host plant tested. Thus, homologous strain-host associations were more effective than heterologous ones. This shows that there is still a high potential for isolating and testing new strains from hybrids to be used as inoculants in the context of large-scale afforestation programs.


Trees ◽  
2012 ◽  
Vol 26 (3) ◽  
pp. 1031-1044
Author(s):  
Clemence Hatt ◽  
François Mankessi ◽  
Jean-Baptiste Durand ◽  
Frédéric Boudon ◽  
Fabienne Montes ◽  
...  

2015 ◽  
Vol 15 (1) ◽  
pp. 72
Author(s):  
Susanti Tasik ◽  
Siti Muslimah Widyastuti ◽  
Harjono .

Mechanism of parasitism of Trichoderma harzianum on Fusarium oxysporum on Acacia mangium seedlings. Fusarium oxysporum is one of the most important soil-borne fungi the causal agent of damping-off disease. Detailed information it needed to know how the pathogen can be inhibited by Trichoderma harzianum. The objective of this research was to investigate the inhibition mechanism of T. harzianum on F. oxysporum in vitro and in planta. Green Flourescent Protein (GFP) T. harzianum was used as biocontrol agent of F. oxysporum. An in vitro inhibition test of T. harzianum was performed using dual culture method. In the in planta inhibition tests, seedlings of A. mangium were applied with GFP T. harzianum two days before inoculation of F. oxysporum; GFP T. harzianum was simultaneously applied with F. oxysporum and GFP T. harzianum was applied two days after inoculation of F. oxysporum. The inhibition effect of T. harzianum GFP was observed at seven days incubation, indicated by attachment of T. harzianum to F. oxysporum hyphae. GFP T. harzianum hyphae covered the colonies of F. oxysporum at 12 days after incubation. The highest life percentage of A. mangium seedlings was found on the treatment of GFP T. harzianum two days before inoculation of F. oxysporum (82.22%), whereas the lowest life percentage was found on seedling applied with GFP T. harzianum two days after inoculation of F. oxysporum (64.44%).


2016 ◽  
Vol 8 (1) ◽  
pp. 412-415 ◽  
Author(s):  
Archana Rani ◽  
M. Kumar ◽  
Sanjeev Kumar

An efficient protocol for callus induction of Withania somnifera through in vitro culture of shoot apex and leaf explant was standardized. Of the various combinations of phytohormones evaluated, MS media supplemented with 6-furfuryl aminopurine (KIN) 0.5 mg/l + 2,4-dichlorophenoxy acetic acid (2, 4-D) 2.0 mg/l was found to be bestfor mean callus induction (86%) in leaf explants after 6 weeks of culture and in case of shoot apex expant the best response and growth of callusing was observed on MS medium supplemented with 2,4-D 1.0 mg/l + BAP 2.0 mg/l (77%).The response of callus growth increases gradually with the reductions in concentration of KIN in culturemedium of both the explants. This protocol might be used in further research for mass propagation of W. somnifera via indirect regeneration methods.


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