Molecular characterization of GPR50 gene and study of its comparative genetic variability in sheep breeds adapted to different thermo-contrasting climatic regimens

2016 ◽  
Vol 61 (4) ◽  
pp. 701-707
Author(s):  
Vijay Kumar Saxena ◽  
Davendra Kumar ◽  
S.M. K Naqvi
Keyword(s):  
Genetic Variability ◽  
Molecular Characterization ◽  
Sheep Breeds

scholarly journals MORPHOLOGICAL, PATHOGENIC AND MOLECULAR CHARACTERIZATION OF Fusarium oxysporum f.sp. ciceri ISOLATES FROM MAHARASHTRA, INDIA

2011 ◽  
Vol 12 (2) ◽  
pp. 47 ◽  
Author(s):  
V.K. Mandhare ◽  
G.P. Deshmukh ◽  
J.V. Patil ◽  
A.A. Kale
Keyword(s):  
Genetic Variability ◽  
Fusarium Oxysporum ◽  
Molecular Characterization ◽  
Pure Culture ◽  
Random Amplified Polymorphic Dna ◽  
Vascular Wilt ◽  
Chick Pea ◽  
Major Factors

Vascular wilt caused by Fusarium oxysporum f.sp. ciceri (FOC) is considered as one of the major factors of low productivity in chickpea. The present study was conducted to determine the morphological, pathogenic and random amplified polymorphic DNA (RAPD) variability of twenty isolates of FOC collected from the Maharashtra State of India, along with four reference isolates corresponding to four known FOC races. Pathogenicity of each isolate was confirmed using the wilt susceptible chick-pea genotype JG-62. The mycelia of all the isolates were septate, hyaline and profusely branched. All the FOC isolates produced micro- and macro-conidia in pure culture within seven days after inoculation. Based on the abilities of the isolates to cause dis-ease on an international set of chickpea differentials and genetic variability estimated by the RAPD technique, these 24 isolates were grouped into two pathotypes, i.e. pathotype I and pathotype II.<br /><br />

scholarly journals Phenotypic and molecular characterization of corn hybrids released from 1980 to 2000

2009 ◽  
Vol 39 (7) ◽  
pp. 1960-1966
Author(s):  
Noryam Bervian Bispo ◽  
Carolina Tessele ◽  
José Fernandes Barbosa Neto
Keyword(s):  
Genetic Variability ◽  
Molecular Characterization ◽  
Kernel Weight ◽  
Phenotypic Traits ◽  
Corn Hybrids ◽  
Corn Breeding ◽  
Tropical Germplasm ◽  
Plant Densities ◽  
Genetic Pool

Genetic variability is the basis of plant breeding. In corn, a large and diverse genetic pool permits manipulation of different genotypes, but the genetic diversity of tropical germplasm has been little studied. With the objective of analyzing the amplitude of the genetic variability in 15 hybrids released in different periods by three different seed companies, phenotypic and molecular characterization was carried out in three environments and at two plant densities. The phenotypic assessment showed great similarity among the hybrids, suggesting that the breeding programs have developed genotypes with similar phenotypic traits. The dendogram of the molecular analysis showed clustering according with the different companies suggesting that each corn breeding program make use of different germoplasms. However, although the germplasm was different, it could be inferred that the selection pressure used by the breeders followed the same traits such as plant height, number of rows per ear and kernel weight among others analyzed in the present study, that contributed to the phenotypic uniformity of the genotype.

scholarly journals The β-casein in camels: molecular characterization of the CSN2 gene, promoter analysis and genetic variability

Gene ◽  
2014 ◽  
Vol 547 (1) ◽  
pp. 159-168 ◽  
Author(s):  
A. Pauciullo ◽  
I.J. Giambra ◽  
L. Iannuzzi ◽  
G. Erhardt
Keyword(s):  
Genetic Variability ◽  
Molecular Characterization ◽  
Promoter Analysis ◽  
Gene Promoter

scholarly journals Morphomolecular characterization of Pleurotus ostreatus (Jacq. Fr.) kummer strains in relation to luminosity and temperature of frutification

2003 ◽  
Vol 60 (3) ◽  
pp. 531-535 ◽  
Author(s):  
Regina Helena Marino ◽  
Augusto Ferreira da Eira ◽  
Eiko Eurya Kuramae ◽  
Elvio Cardoso Queiroz
Keyword(s):  
Genetic Variability ◽  
Molecular Characterization ◽  
Pleurotus Ostreatus ◽  
Random Amplified Polymorphic Dna ◽  
Factors Affecting ◽  
Formation Pattern ◽  
Main Factors ◽  
Effects Of Temperature ◽  
Rapd Method

Temperature is one of the main factors affecting mushrooms development and introduction in new areas. Effects of temperature (15ºC and 28ºC) and luminosity (120 and 900 lux) were evaluated for eight P. ostreatus strains in relation to precocity, yield, pileus area, stalk formation pattern, coloration and handling resistance. Genetic variability of strains was analysed by the Random Amplified Polymorphic DNA (RAPD) method. The Pos 98/37 strain was the only to yield white pileus at 28ºC - 900 lux, and grey ones at 15ºC and 120 lux. The Pos 96/05 strain, the latest, produced lead-coloured pileus at 15ºC, as did the remaining strains at this temperature. Strains cultivated at 15ºC did not differ in relation to handling resistance. At 28ºC mushrooms were less resistant. In relation to yield, the Pos 98/38 strain was significantly more efficient. The Pos 98/37 strain, at 28ºC, as compared to the same strain at 15ºC, was more efficient and had an asymmetric stalk formation pattern. Among strains cultivated at 15ºC, the stalk formation pattern was symmetric, except for the Pos 97/15 and Pos 97/17 strains. Molecular characterization of the Pos 98/37 strain was 30% similar to the remaining strains. The temperature of fructification and luminosity influence the induction and development of the isolates.

Characterization of MTNR1A gene in terms of genetic variability in a panel of subtemperate and subtropical Indian sheep breeds

2015 ◽  
Vol 94 (4) ◽  
pp. 715-721 ◽  
Author(s):  
VIJAY KUMAR SAXENA ◽  
BIPUL KUMAR JHA ◽  
AMAR SINGH MEENA ◽  
S. M. K. NAQVI
Keyword(s):  
Genetic Variability ◽  
Sheep Breeds ◽  
Indian Sheep

Molecular characterization of A1/A2 Beta-casein Alleles in Vrindavani crossbred and Sahiwal cattle

2018 ◽  
Author(s):  
Sushil Kumar ◽  
Ram Vir Singh ◽  
Anuj Chauhan
Keyword(s):  
Genetic Variability ◽  
Molecular Characterization ◽  
Allelic Frequency ◽  
Genotypic Frequency ◽  
Allelic Frequencies ◽  
Genotype Frequencies ◽  
Sahiwal Cattle ◽  
Sahiwal Cows ◽  
Beta Casein

In the present investigation Vrindavani cross bred and indigenous Sahiwal cows were genotyped for A1/ A2 b-casein alleles. Present genotypic study on A1/A2 Beta-casein variants was conducted in a population of 660 cattle which comprises of 354 Vrindavani and 306 Sahiwal cows. Out of them 354 Vrindavani cows were maintained at Cattle and Buffalo Farm, IVRI, Izatnagar and 306 Sahiwal cows under study were maintained at Kamdhenu Gaushala, Noormahal, Jalandhar. In Vrindavani cows, all three type of genotype were observed viz., A1A1, A1A2 and A2A2. Genotypic frequencies of A1A1, A1A2 and A2A2 genotypes were 0.11, 0.47 and 0.42 respectively. The frequency of A1 and A2 allelic frequency in this population was 0.35 and 0.65 respectively in Vrindavani cows. In Sahiwal cows, only two types of genotypes were observed viz., A1A2 and A2A2. Genotype frequencies for A1A2 and A2A2 genotypes were 0.13 and 0.87 in Sahiwal cattle. The allelic frequencies for A1 and A2 were 0.06 and 0.94 respectively in Sahiwal cows. The results revealed that maximum genotypic frequency observed was A1A2 (0.47) followed by A2A2 (0.42) and A1A1 (0.11) in Vrindavani cattle while in Sahiwal cattle maximum genotypic frequency of A2A2 (0.87) followed by A1A2 (0.13) respectively. The observed heterozygosity (0.47) and PIC value (0.35) pointed towards the existence of medium genetic variability in the tested population.

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