scholarly journals Morphomolecular characterization of Pleurotus ostreatus (Jacq. Fr.) kummer strains in relation to luminosity and temperature of frutification

2003 ◽  
Vol 60 (3) ◽  
pp. 531-535 ◽  
Author(s):  
Regina Helena Marino ◽  
Augusto Ferreira da Eira ◽  
Eiko Eurya Kuramae ◽  
Elvio Cardoso Queiroz
Keyword(s):  
Genetic Variability ◽  
Molecular Characterization ◽  
Pleurotus Ostreatus ◽  
Random Amplified Polymorphic Dna ◽  
Factors Affecting ◽  
Formation Pattern ◽  
Main Factors ◽  
Effects Of Temperature ◽  
Rapd Method

Temperature is one of the main factors affecting mushrooms development and introduction in new areas. Effects of temperature (15ºC and 28ºC) and luminosity (120 and 900 lux) were evaluated for eight P. ostreatus strains in relation to precocity, yield, pileus area, stalk formation pattern, coloration and handling resistance. Genetic variability of strains was analysed by the Random Amplified Polymorphic DNA (RAPD) method. The Pos 98/37 strain was the only to yield white pileus at 28ºC - 900 lux, and grey ones at 15ºC and 120 lux. The Pos 96/05 strain, the latest, produced lead-coloured pileus at 15ºC, as did the remaining strains at this temperature. Strains cultivated at 15ºC did not differ in relation to handling resistance. At 28ºC mushrooms were less resistant. In relation to yield, the Pos 98/38 strain was significantly more efficient. The Pos 98/37 strain, at 28ºC, as compared to the same strain at 15ºC, was more efficient and had an asymmetric stalk formation pattern. Among strains cultivated at 15ºC, the stalk formation pattern was symmetric, except for the Pos 97/15 and Pos 97/17 strains. Molecular characterization of the Pos 98/37 strain was 30% similar to the remaining strains. The temperature of fructification and luminosity influence the induction and development of the isolates.

scholarly journals MORPHOLOGICAL, PATHOGENIC AND MOLECULAR CHARACTERIZATION OF Fusarium oxysporum f.sp. ciceri ISOLATES FROM MAHARASHTRA, INDIA

2011 ◽  
Vol 12 (2) ◽  
pp. 47 ◽  
Author(s):  
V.K. Mandhare ◽  
G.P. Deshmukh ◽  
J.V. Patil ◽  
A.A. Kale
Keyword(s):  
Genetic Variability ◽  
Fusarium Oxysporum ◽  
Molecular Characterization ◽  
Pure Culture ◽  
Random Amplified Polymorphic Dna ◽  
Vascular Wilt ◽  
Chick Pea ◽  
Major Factors

Vascular wilt caused by Fusarium oxysporum f.sp. ciceri (FOC) is considered as one of the major factors of low productivity in chickpea. The present study was conducted to determine the morphological, pathogenic and random amplified polymorphic DNA (RAPD) variability of twenty isolates of FOC collected from the Maharashtra State of India, along with four reference isolates corresponding to four known FOC races. Pathogenicity of each isolate was confirmed using the wilt susceptible chick-pea genotype JG-62. The mycelia of all the isolates were septate, hyaline and profusely branched. All the FOC isolates produced micro- and macro-conidia in pure culture within seven days after inoculation. Based on the abilities of the isolates to cause dis-ease on an international set of chickpea differentials and genetic variability estimated by the RAPD technique, these 24 isolates were grouped into two pathotypes, i.e. pathotype I and pathotype II.<br /><br />

scholarly journals MORPHOLOGICAL, PATHOGENIC AND MOLECULAR CHARACTERIZATION OF Fusarium oxysporum f.sp. ciceri ISOLATES FROM MAHARASHTRA, INDIA

2011 ◽  
Vol 12 (2) ◽  
pp. 47
Author(s):  
V.K. Mandhare ◽  
G.P. Deshmukh ◽  
J.V. Patil ◽  
A.A. Kale
Keyword(s):  
Genetic Variability ◽  
Fusarium Oxysporum ◽  
Molecular Characterization ◽  
Pure Culture ◽  
Random Amplified Polymorphic Dna ◽  
Vascular Wilt ◽  
Chick Pea ◽  
Major Factors

Vascular wilt caused by Fusarium oxysporum f.sp. ciceri (FOC) is considered as one of the major factors of low productivity in chickpea. The present study was conducted to determine the morphological, pathogenic and random amplified polymorphic DNA (RAPD) variability of twenty isolates of FOC collected from the Maharashtra State of India, along with four reference isolates corresponding to four known FOC races. Pathogenicity of each isolate was confirmed using the wilt susceptible chick-pea genotype JG-62. The mycelia of all the isolates were septate, hyaline and profusely branched. All the FOC isolates produced micro- and macro-conidia in pure culture within seven days after inoculation. Based on the abilities of the isolates to cause dis-ease on an international set of chickpea differentials and genetic variability estimated by the RAPD technique, these 24 isolates were grouped into two pathotypes, i.e. pathotype I and pathotype II.<br /><br />

Dissolution of Zirconium Power and Detection of Zirconium Powder Content by ICP-AES Method

2014 ◽  
Vol 936 ◽  
pp. 1571-1575
Author(s):  
Xiao Lian Zhang ◽  
Wei Hua Zhu ◽  
Xin Zheng ◽  
Hong Xia Liu
Keyword(s):  
Atomic Emission ◽  
Deionized Water ◽  
Analysis Method ◽  
Plasma Atomic Emission ◽  
Factors Affecting ◽  
Instrument Analysis ◽  
Main Factors ◽  
Effects Of Temperature ◽  
Powder Content ◽  
Zirconium Powder

Instrument analysis method is used in this experiment, which detecting the zirconium content of zirconium powder by using plasma-atomic emission spectrometer. In this experiment, the instrumental detective conditions were determined, and analyzed several main factors affecting the experimental results, concluded the effects of temperature, hydrofluoric acid and deionized water on dissolving zirconium powder, summarized the method to detect zirconium powder content directly by ICP.

scholarly journals Genetic characterization of the Moxotó goat breed using RAPD markers

2005 ◽  
Vol 40 (3) ◽  
pp. 233-239 ◽  
Author(s):  
Rejane Rodrigues de Oliveira ◽  
Andréa Alves do Egito ◽  
Maria Norma Ribeiro ◽  
Samuel Rezende Paiva ◽  
Maria do Socorro Maués Albuquerque ◽  
...  
Keyword(s):  
Genetic Variability ◽  
Rapd Markers ◽  
Genetic Characterization ◽  
Random Amplified Polymorphic Dna ◽  
Genetic Distances ◽  
Goat Breed ◽  
Breeding Programs ◽  
Analysis Of Molecular Variance ◽  
Polymorphic Bands

The objective of this study was to verify the genetic diversity between and within seven populations of Moxotó goat (n = 264) from the States of Pernambuco, Paraíba and Rio Grande do Norte, using RAPD (Random Amplified Polymorphic DNA). Moxotó, as well as other naturalized breeds, suffers genetic losses due to the indiscriminate miscegenation with breeds raised in the Northeast Region of Brazil. The genetic characterization of these genetic resources is essential to conservation and breeding programs. DNA was extracted from lymphocytes using a non-organic protocol. The 16 primers used were selected from 120 decamer oligonucleotide primers and generated 56 polymorphic bands. The analysis of molecular variance (AMOVA) showed that the greater part of total genetic variability (71.55%) was due to differences between individuals within populations, while 21.21% was among populations. The analysis of variance among the pairs of populations demonstrated that the populations located in Floresta, PE x Angicos, RN presented a smaller value of intrapopulational differentiation (8.9%), indicating low genetic variability among them. Nei's genetic distances varied between 0.0546 and 0.1868 in the populations. The dendrogram generated showed that the Canindé breed, used as outgroup, clustered with the populations of Moxotó, indicating a possible common origin of the naturalized goat breeds.

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