Drug sensitivity of Plasmodium falciparum along the Thai–Myanmar border using the new field-deployable HRP2 in vitro assay

2005 ◽  
Vol 117 (S4) ◽  
pp. 35-38 ◽  
Author(s):  
Bernhard Attlmayr ◽  
Herwig Kollaritsch ◽  
Walther H. Wernsdorfer ◽  
Robert S. Miller ◽  
Jeeraphat Sirichaisinthop ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Drug Sensitivity ◽  
In Vitro Assay ◽  
Vitro Assay

scholarly journals Influence of oxygen on asexual blood cycle and susceptibility of Plasmodium falciparum to chloroquine: requirement of a standardized in vitro assay

2007 ◽  
Vol 6 (1) ◽  
Author(s):  
Sébastien Briolant ◽  
Philippe Parola ◽  
Thierry Fusaï ◽  
Marilyn Madamet-Torrentino ◽  
Eric Baret ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
In Vitro Assay ◽  
Vitro Assay

scholarly journals Plasmodium falciparum: Assessment of Selectivity of Action of Chloroquine, Alchornea cordifolia, Ficus polita, and Other Drugs by a Tetrazolium-Based Colorimetric Assay

2011 ◽  
Vol 2011 ◽  
pp. 1-7 ◽  
Author(s):  
Nana Kofi Ayisi ◽  
Regina Appiah-Opong ◽  
Ben Gyan ◽  
Kwasi Bugyei ◽  
Fred Ekuban
Keyword(s):  
Plasmodium Falciparum ◽  
Red Blood Cells ◽  
Incubation Time ◽  
Blood Cells ◽  
Colorimetric Assay ◽  
In Vitro Assay ◽  
Effective Concentration ◽  
Vitro Assay ◽  
Number Of Cells

A tetrazolium-based colorimetric selective assay (MTT-based CSA) was developed to assess the selectivity of antimalarial drugs. This in vitro assay, unlike all others, measures the ability of drugs to indirectly protect red blood cells (RBCs) from Plasmodium-falciparum-induced destruction. Optimum incubation time and number of cells needed were 5 days and 23×106 RBCs per well, respectively. A parasitemia range of 0.375% to 3% was found to be suitable for this assay. The MTT-based CSA determined anti-P. falciparum strain DD2 activity of chloroquine at a higher 50% effective concentration (EC50) value (21.0 μg/mL) than the isotopic microtest (10.0 μg/mL). Artesunate and oxytetracycline achieved 90% effect against DD2 with minimal or no toxicity to RBCs. Against chloroquine sensitive strain 3D7, chloroquine and Alchornea cordifolia had EC50 values of 0.025 μg/mL and 4.9 μg/mL respectively, and selective index (SI) values of >2,000 and >69.4 μg/mL, respectively.

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