Fluorometric determination of Simian virus 40 based on strand displacement amplification and triplex DNA using a molecular beacon probe with a guanine-rich fragment of the stem region

2016 ◽  
Vol 184 (2) ◽  
pp. 557-562 ◽  
Author(s):  
Yubin Li ◽  
Ruimin Li ◽  
Li Zou ◽  
Manjun Zhang ◽  
Liansheng Ling
Keyword(s):  
Molecular Beacon ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
Triplex Dna ◽  
Strand Displacement ◽  
Fluorometric Determination ◽  
Strand Displacement Amplification

Fluorometric determination of microRNA based on strand displacement amplification and rolling circle amplification

2017 ◽  
Vol 184 (11) ◽  
pp. 4359-4365 ◽  
Author(s):  
Yunlei Zhou ◽  
Bingchen Li ◽  
Minghui Wang ◽  
Jun Wang ◽  
Huanshun Yin ◽  
...  
Keyword(s):  
Rolling Circle Amplification ◽  
Strand Displacement ◽  
Fluorometric Determination ◽  
Strand Displacement Amplification ◽  
Rolling Circle

Method of mapping DNA replication origins

1985 ◽  
Vol 5 (1) ◽  
pp. 85-92
Author(s):  
L D Spotila ◽  
J A Huberman
Keyword(s):  
Dna Replication ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
Replication Forks ◽  
Replication Origins ◽  
Chromosomal Dna ◽  
Dna Restriction Fragments ◽  
Dna Restriction ◽  
Dna Replication Origins

We have developed a method which allows determination of the direction in which replication forks move through segments of chromosomal DNA for which cloned probes are available. The method is based on the facts that DNA restriction fragments containing replication forks migrate more slowly through agarose gels than do non-fork-containing fragments and that the extent of retardation of the fork-containing fragments is a function of the extent of replication. The procedure allows the identification of DNA replication origins as sites from which replication forks diverge. In this paper we demonstrate the feasibility of this procedure, with simian virus 40 DNA as a model, and we discuss its applicability to other systems.

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