A SYBR Green-based real-time RT-PCR assay for simple and rapid detection and differentiation of highly pathogenic and classical type 2 porcine reproductive and respiratory syndrome virus circulating in China

2012 ◽  
Vol 158 (2) ◽  
pp. 407-415 ◽  
Author(s):  
Zheng Chai ◽  
Wenjun Ma ◽  
Fang Fu ◽  
Yuekun Lang ◽  
Wei Wang ◽  
...  
Keyword(s):  
Real Time ◽  
Rapid Detection ◽  
Sybr Green ◽  
Respiratory Syndrome Virus ◽  
Classical Type ◽  
Rt Pcr ◽  
Pcr Assay ◽  
Highly Pathogenic

scholarly journals Development of a SYBR green-based real-time RT-PCR assay for rapid detection of the emerging swine acute diarrhea syndrome coronavirus

2019 ◽  
Vol 265 ◽  
pp. 66-70 ◽  
Author(s):  
Lei Ma ◽  
Fanwen Zeng ◽  
Feng Cong ◽  
Bihong Huang ◽  
Ren Huang ◽  
...  
Keyword(s):  
Real Time ◽  
Acute Diarrhea ◽  
Rapid Detection ◽  
Sybr Green ◽  
Rt Pcr ◽  
Pcr Assay ◽  
Diarrhea Syndrome

scholarly journals A Broadly Reactive One-Step SYBR Green I Real-Time RT-PCR Assay for Rapid Detection of Murine Norovirus

PLoS ONE ◽  
2014 ◽  
Vol 9 (5) ◽  
pp. e98108 ◽  
Author(s):  
Ken-Ichi Hanaki ◽  
Fumio Ike ◽  
Ayako Kajita ◽  
Wataru Yasuno ◽  
Misato Yanagiba ◽  
...  
Keyword(s):  
Real Time ◽  
Rapid Detection ◽  
Sybr Green ◽  
Sybr Green I ◽  
Murine Norovirus ◽  
Rt Pcr ◽  
Pcr Assay ◽  
One Step

scholarly journals A real-time RT-PCR assay for rapid detection of coxsackievirus A10

2015 ◽  
Vol 14 (4) ◽  
pp. 17496-17504 ◽  
Author(s):  
C.Y. Mu ◽  
A.Y. Wang ◽  
C. Chen ◽  
L. Zhao ◽  
Z. Li
Keyword(s):  
Real Time ◽  
Rapid Detection ◽  
Rt Pcr ◽  
Pcr Assay

scholarly journals Design and evaluation of a unique SYBR Green real-time RT-PCR assay for quantification of five major cytokines in cattle, sheep and goats

2015 ◽  
Vol 11 (1) ◽  
pp. 65 ◽  
Author(s):  
Carinne Puech ◽  
Laurence Dedieu ◽  
Isabelle Chantal ◽  
Valérie Rodrigues
Keyword(s):  
Real Time ◽  
Sybr Green ◽  
Rt Pcr ◽  
Pcr Assay ◽  
Sheep And Goats

Development and evaluation of SYBR Green I-based one-step real-time RT-PCR assay for detection and quantitation of Japanese encephalitis virus

2007 ◽  
Vol 143 (1) ◽  
pp. 73-80 ◽  
Author(s):  
S.R. Santhosh ◽  
M.M. Parida ◽  
P.K. Dash ◽  
A. Pateriya ◽  
B. Pattnaik ◽  
...  
Keyword(s):  
Japanese Encephalitis Virus ◽  
Real Time ◽  
Japanese Encephalitis ◽  
Sybr Green ◽  
Encephalitis Virus ◽  
Sybr Green I ◽  
Rt Pcr ◽  
Pcr Assay ◽  
One Step

A Duplex SYBR Green I-based Real-time Polymerase Chain Reaction Assay for Rapid Detection of Canine Kobuvirus and Canine Circovirus

2021 ◽  
Author(s):  
Yang Pan ◽  
Jing Chen ◽  
Junhuang Wu ◽  
Yongxia Wang ◽  
Junwei Zou ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Rapid Detection ◽  
Simultaneous Detection ◽  
Sybr Green ◽  
Sybr Green I ◽  
Chain Reaction ◽  
Pcr Assay ◽  
Polymerase Chain ◽  
Canine Kobuvirus

Abstract Background: Canine Kobuvirus (CaKoV) and Canine Circovirus (CaCV) are viruses that infect dogs causing diarrheal symptoms that are very similar. However, there is no clinical method to detect a co-infection of these two viruses.Results: In this study, a duplex SYBR Green I-based quantitative real-time polymerase chain reaction (PCR) assay for the rapid and simultaneous detection of CaKoV and CaCV was established. CaKoV and CaCV were distinguished by their different melting temperature which was 86℃ for CaKoV and 78℃ for CaCV. The assay was highly specific, with no cross-reactivity with other common canine viruses and demonstrated high sensitivity. The detection limits of CaKoV and CaCV were 8.924 × 101 copies/μL and 3.841 × 101 copies/μL, respectively. The highest intra- and inter-assay Ct value variation coefficients (CV) of CaKoV were 0.40% and 0.96%, respectively. For CaCV, the highest intra- and inter-assay Ct value variation coefficients were 0.26% and 0.70%, respectively. In 57 clinical samples, positive detection rates of CaKoV and CaCV were 8.77% (7/57) and 15.79% (9/57), respectively. The co-infection rate was 7.02% (4/57). Conclusions: The duplex SYBR Green I-based real-time PCR assay established in this study is a fast, efficient, and sensitive method for the simultaneous detection of the two viruses and provides a powerful tool for the rapid detection of CaKoV and CaCV in clinical practice.

A TaqMan-MGB real-time RT-PCR assay with an internal amplification control for rapid detection of Muscovy duck reovirus

2020 ◽  
Vol 52 ◽  
pp. 101575
Author(s):  
Min Zheng ◽  
Xiuqin Chen ◽  
Shao Wang ◽  
Jingxiang Wang ◽  
Meiqing Huang ◽  
...  
Keyword(s):  
Real Time ◽  
Rapid Detection ◽  
Internal Amplification Control ◽  
Muscovy Duck ◽  
Rt Pcr ◽  
Pcr Assay ◽  
Muscovy Duck Reovirus

Development of an EvaGreen based real-time RT-PCR assay for rapid detection, quantitation and diagnosis of goose calicivirus

2020 ◽  
Vol 49 ◽  
pp. 101489 ◽  
Author(s):  
Su Lin ◽  
Shizhong Zhang ◽  
Shao Wang ◽  
Kaichun Xie ◽  
Dandan Jiang ◽  
...  
Keyword(s):  
Real Time ◽  
Rapid Detection ◽  
Rt Pcr ◽  
Pcr Assay

Rapid detection of Enterovirus and Coxsackievirus A10 by a TaqMan based duplex one-step real time RT-PCR assay

2017 ◽  
Vol 33 ◽  
pp. 8-10 ◽  
Author(s):  
Jingfang Chen ◽  
Rusheng Zhang ◽  
Xinhua Ou ◽  
Dong Yao ◽  
Zheng Huang ◽  
...  
Keyword(s):  
Real Time ◽  
Rapid Detection ◽  
Rt Pcr ◽  
Pcr Assay ◽  
One Step

scholarly journals Multiplex real-time RT-PCR assay for bovine viral diarrhea virus type 1, type 2 and HoBi-like pestivirus

2016 ◽  
Vol 229 ◽  
pp. 1-7 ◽  
Author(s):  
Viviana Mari ◽  
Michele Losurdo ◽  
Maria Stella Lucente ◽  
Eleonora Lorusso ◽  
Gabriella Elia ◽  
...  
Keyword(s):  
Real Time ◽  
Bovine Viral Diarrhea Virus ◽  
Bovine Viral Diarrhea ◽  
Rt Pcr ◽  
Pcr Assay ◽  
Diarrhea Virus ◽  
Viral Diarrhea ◽  
Viral Diarrhea Virus
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