Cellular change and callose accumulation in zygotic embryos of Eleutherococcus senticosus caused by plasmolyzing pretreatment result in high frequency of single-cell-derived somatic embryogenesis

The γ-aminobutyric acid (Gaba) is a non-protein amino acid found in prokaryotes and eukaryotes. Its role in plant development has not been fully established. This study reports a quantification of the levels of endogenous Gaba, as well as investigation of its role in different stages of somatic embryogenesis in Acca sellowiana Berg. (Myrtaceae). Zygotic embryos were used as explants and they were inoculated into the culture medium contained different concentrations of Gaba (0,2, 4, 6, 8 and 10 µM). The highest concentrations of endogenous Gaba were detected between the third and nine days after inoculation, reaching the value of 12.77 µmol.g-1FW. High frequency of somatic embryogenesis was observed in response to 10 µM Gaba. This treatment also resulted in a large number of normal embryos, and the lowest percentage of formation of fused somatic embryos, phenotypic characteristic of most deformed embryos in all treatments. Also, all treatments promoted the formation of the somatic embryos with positive characteristics of development resumption, which however did not originate the seedlings.

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High-frequency plantlet regeneration by somatic embryogenesis from mature zygotic embryos of onion

New Zealand Journal of Crop and Horticultural Science ◽

10.1080/01140671.2015.1049621 ◽

2015 ◽

Vol 43 (4) ◽

pp. 249-260 ◽

Cited By ~ 2

Author(s):

X Wu ◽

F Yang ◽

XC Piao ◽

KH Li ◽

ML Lian ◽

...

Keyword(s):

Somatic Embryogenesis ◽

High Frequency ◽

Plantlet Regeneration ◽

Zygotic Embryos

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Effect of 2, 4-D and Picloram on Somatic Embryogenesis in Carica papaya var. P-7-9

Plant Tissue Culture and Biotechnology ◽

10.3329/ptcb.v29i1.41976 ◽

2019 ◽

Vol 29 (1) ◽

pp. 25-32

Author(s):

Kulbhushan Chaudhary ◽

Jai Prakash

Keyword(s):

Somatic Embryogenesis ◽

High Frequency ◽

Somatic Embryos ◽

Carica Papaya ◽

Zygotic Embryos ◽

Shoot Induction ◽

Free Medium ◽

Embryo Germination ◽

Shoot Bud ◽

Half Strength

Immature zygotic embryos of Carica papaya L. var. P-7-9 were inoculated on half-strength MS with different concentrations of 2,4-D and picloram alone and their combinations. The highest induction (80%) of somatic embryogenesis was MS supplemented with 2,4-D (7.0 mg/l) followed by 2,4-D (4.0 mg/l) + picloram (1.0 mg/l) but no embryogenesis was observed in auxin-free medium. The addition of osmoticum such as PEG and ABA significantly increased the maturation of somatic embryos. BAP, TDZ and NAA were used for shoot induction. Combination of 2.0 mg/l BAP and 0.5 mg/l NAA was found significantly better for shoot bud proliferation and 70% multiplication. The percentage of germinating embryos was enhanced significantly when the MS fortified with GA3 (0.5 mg/l). However, the high frequency of embryo germination and plantlets formation were obtained with 2,4-D (4.0 mg/l) + picloram (1.0 mg/l). Plant Tissue Cult. & Biotech. 29(1): 25-32, 2019 (June)

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