Polyamines increase nitric oxide and reactive oxygen species in guard cells of Arabidopsis thaliana during stomatal closure

PROTOPLASMA ◽  
2017 ◽  
Vol 255 (1) ◽  
pp. 153-162 ◽  
Author(s):  
Srinivas Agurla ◽  
Gunja Gayatri ◽  
Agepati S. Raghavendra
Keyword(s):  
Nitric Oxide ◽  
Reactive Oxygen Species ◽  
Arabidopsis Thaliana ◽  
Stomatal Closure ◽  
Reactive Oxygen ◽  
Guard Cells ◽  
Oxygen Species

Ethylene mediates salicylic-acid-induced stomatal closure by controlling reactive oxygen species and nitric oxide production in Arabidopsis

Plant Science ◽  
2020 ◽  
Vol 294 ◽  
pp. 110464 ◽  
Author(s):  
Hui-Qin Wang ◽  
Li-Ping Sun ◽  
Li-Xiao Wang ◽  
Xiao-Wei Fang ◽  
Zhong-Qi Li ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Reactive Oxygen Species ◽  
Salicylic Acid ◽  
Stomatal Closure ◽  
Reactive Oxygen ◽  
Nitric Oxide Production ◽  
Oxygen Species ◽  
Oxide Production

scholarly journals Reactive Oxygen Species-Dependent Nitric Oxide Production Contributes to Hydrogen-Promoted Stomatal Closure in Arabidopsis

2014 ◽  
Vol 165 (2) ◽  
pp. 759-773 ◽  
Author(s):  
Yanjie Xie ◽  
Yu Mao ◽  
Wei Zhang ◽  
Diwen Lai ◽  
Qingya Wang ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Reactive Oxygen Species ◽  
Stomatal Closure ◽  
Reactive Oxygen ◽  
Nitric Oxide Production ◽  
Oxygen Species ◽  
Oxide Production

scholarly journals Autophagy controls reactive oxygen species homeostasis in guard cells that is essential for stomatal opening

2019 ◽  
Vol 116 (38) ◽  
pp. 19187-19192 ◽  
Author(s):  
Shota Yamauchi ◽  
Shoji Mano ◽  
Kazusato Oikawa ◽  
Kazumi Hikino ◽  
Kosuke M. Teshima ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Stomatal Closure ◽  
Reactive Oxygen ◽  
Guard Cells ◽  
Stomatal Opening ◽  
Glycolate Oxidase ◽  
Oxygen Species ◽  
Ros Accumulation ◽  
Intracellular Ros ◽  
Ros Scavenger

Reactive oxygen species (ROS) function as key signaling molecules to inhibit stomatal opening and promote stomatal closure in response to diverse environmental stresses. However, how guard cells maintain basal intracellular ROS levels is not yet known. This study aimed to determine the role of autophagy in the maintenance of basal ROS levels in guard cells. We isolated the Arabidopsis autophagy-related 2 (atg2) mutant, which is impaired in stomatal opening in response to light and low CO2 concentrations. Disruption of other autophagy genes, including ATG5, ATG7, ATG10, and ATG12, also caused similar stomatal defects. The atg mutants constitutively accumulated high levels of ROS in guard cells, and antioxidants such as ascorbate and glutathione rescued ROS accumulation and stomatal opening. Furthermore, the atg mutations increased the number and aggregation of peroxisomes in guard cells, and these peroxisomes exhibited reduced activity of the ROS scavenger catalase and elevated hydrogen peroxide (H2O2) as visualized using the peroxisome-targeted H2O2 sensor HyPer. Moreover, such ROS accumulation decreased by the application of 2-hydroxy-3-butynoate, an inhibitor of peroxisomal H2O2-producing glycolate oxidase. Our results showed that autophagy controls guard cell ROS homeostasis by eliminating oxidized peroxisomes, thereby allowing stomatal opening.

scholarly journals Differences between apoplastic and cytosolic reactive oxygen species production in Arabidopsis during pattern-triggered immunity

2021 ◽  
Author(s):  
Dominique Arnaud ◽  
Michael J. Deeks ◽  
Nicholas Smirnoff
Keyword(s):  
Reactive Oxygen Species ◽  
Hydrogen Peroxide ◽  
Cell Wall ◽  
Stomatal Closure ◽  
Reactive Oxygen ◽  
Guard Cells ◽  
Oxygen Species ◽  
Nadph Oxidases ◽  
Molecular Patterns ◽  
Intracellular Hydrogen Peroxide

AbstractDespite an ever-increasing interest in reactive oxygen species (ROS) signalling during plant-microbe interactions, very little information exists, mainly for technical reasons, on the molecular mechanisms regulating intracellular hydrogen peroxide (H2O2) signalling during PAMP-triggered immunity. Here, we used a sensitive fluorimetry method and the H2O2 sensor roGFP2-Orp1, which revealed unsuspected features on the regulation of cytoplasmic H2O2 and thiol redox dynamics upon pathogen-associated molecular patterns (PAMPs) perception by Arabidopsis thaliana. Extended PAMP-induced cytosolic roGFP2-Orp1 oxidation was distinct from the transient oxidative burst in the apoplast measured by luminol oxidation. Pharmacological and genetic analyses indicate that the prolonged PAMP-induced H2O2 increase in the cytoplasm was largely independent on NADPH oxidases and apoplastic peroxidases. By contrast, the NADPH oxidase mutant rbohF was hyper-sensitive to roGFP2-Orp1 oxidation by H2O2 and PAMP indicating a lower antioxidant capacity. Unlike previous reports, the rbohF mutant, but not rbohD, was impaired in PAMP-triggered stomatal closure and ROS production measured by a fluorescein-based probe in guard cells resulting in defects in stomatal defences against bacteria. However, stomatal closure was not correlated with an increase in roGFP2-Orp1 oxidation in guard cells. Interestingly, RBOHF also participated in PAMP-induced apoplastic alkalinisation. Altogether, our results provide novel insights on the interplay between apoplastic and cytosolic ROS dynamics and highlight the importance of RBOHF in plant immunity.Significance statementPlants mount defence responses to pathogens by detecting pathogen-associated molecular patterns (PAMPs). One response is a rapid and transient burst of reactive oxygen species (ROS, e.g. superoxide and hydrogen peroxide) in the cell wall (apoplast) produced by NADPH oxidases and cell wall peroxidases. Using a genetically-encoded hydrogen peroxide sensor roGFP2-Orp1, we found that, in contrast to the transient apoplastic ROS burst, there is also prolonged hydrogen peroxide production in the cytosol upon PAMP perception which is independent of NADPH oxidase and cell wall peroxidases. Our results suggest that apoplastic ROS rather than intracellular hydrogen peroxide is a signal triggering stomatal closure during PAMP-triggered immunity. Additionally, we re-address the relative contribution of the NADPH oxidases D and F in stomatal immunity.

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