High-performance thin-layer chromatographic method development and determination of bio-enhancer from Piper trichostachyon: an ethnomedicinal plant

2021 ◽  
Author(s):  
Pramod J. Hurkadale ◽  
Shrikrishna M. Nandanwadkar ◽  
Chaitrali M. Bidikar ◽  
Rutuja N. Patil ◽  
Harsha V. Hegde
Keyword(s):  
Thin Layer ◽  
High Performance ◽  
Chromatographic Method ◽  
Method Development ◽  
Ethnomedicinal Plant

scholarly journals A NEW HIGH PERFORMANCE THIN LAYER CHROMATOGRAPHIC METHOD DEVELOPMENT AND VALIDATION OF DAPAGLIFLOZIN IN BULK AND TABLET DOSAGE FORM

2019 ◽  
pp. 58-63
Author(s):  
B. V. SUMA ◽  
DEVESWARAN R. ◽  
PREMNATH SHENOY
Keyword(s):  
Thin Layer ◽  
High Performance ◽  
Dosage Form ◽  
Chromatographic Method ◽  
Method Development ◽  
Retention Factor ◽  
Accurate Analysis ◽  
Development And Validation ◽  
Tablet Dosage

ABSTRACT Objective: To develop a new simple, selective and precise high-performance thin layer chromatographic method for determination of Dapagliflozin (DAPA) in bulk and tablet dosage form Methods: The present study describes development and validation of High performance thin layer chromatographic method for DAPA. The chromatographic separation was carried out on Merck precoated silica gel aluminium plate 60 F254 using Chloroform: Methanol (9:1v/v) as mobile phase. Quantitative determination of drug was carried out by densitometric scanning of plates at 223 nm using Camag TLC Scanner. Results: The chromatographic condition shows compact band with the retention factor for dapaglifloxin as 0.21 ± 0.004. The method was validated as per ICH guidelines for linearity, accuracy, precision and robustness. Response was found to be linear in the concentration range of 400 ng/ band to 1200 ng/band with linear regression value of 0.9953 with respect to peak area and concentration value The LOD and LOQ was found to be 1.2083 ng/band and 3.6616ng/ band. The percentage assay was found to be 100±0.05. Conclusion: This method under statistical analysis proved a selective, repeatable and accurate analysis of the drug. This method can be used for quantitative analysis of dapaglifloxin in the bulk drug and in tablet.    

Validated high-performance thin-layer chromatographic method for the determination of dibenzyl cyclooctadiene lignans fromSchisandra grandiflora

2014 ◽  
Vol 27 (6) ◽  
pp. 460-465 ◽  
Author(s):  
Katragunta Kumar ◽  
Bokka Ramesh ◽  
Vadaparthi Rao ◽  
Borra Poornima ◽  
Vanka Sarma ◽  
...  
Keyword(s):  
Thin Layer ◽  
High Performance ◽  
Chromatographic Method

Rapid Thin Layer Chromatographic Method for Determination of Zearalenone and Zearalenol in Grains and Animal Feeds

1984 ◽  
Vol 67 (3) ◽  
pp. 580-582 ◽  
Author(s):  
Steven P Swanson ◽  
Richard A Corley ◽  
Donald G White ◽  
William B Buck
Keyword(s):  
Thin Layer ◽  
Ethyl Acetate ◽  
Salt Solution ◽  
Lead Acetate ◽  
High Performance ◽  
Chromatographic Method ◽  
Inexpensive Method ◽  
Animal Feeds ◽  
Better Than

Abstract A rapid and inexpensive method has been developed for the analysis of zearalenone and zearalenol in grains and animal feeds. The method involves extraction with 75% methanol, precipitation of pigments with lead acetate, and defatting with petroleum ether. The mycotoxins are subsequently partitioned into toluene–ethyl acetate, chromatographed on high performance thin layer chromatographic plates, and detected after treatment with Fast Violet B salt solution. Sensitivity of the method is better than 80 ng/g for zearalenone and 200 ng/g for zearalenol. Ten samples can be completed in less than 2 h. The method is applicable for zearalenone in corn, wheat, barley, millet, and swine feeds.

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