scholarly journals Recycled ionic liquid 1-ethyl-3-methylimidazolium acetate pretreatment for enhancing enzymatic saccharification of softwood without cellulose regeneration

2017 ◽  
Vol 64 (2) ◽  
pp. 149-156 ◽  
Author(s):  
Umi Hamidah ◽  
Takuya Arakawa ◽  
Yin Ying H’ng ◽  
Akiko Nakagawa-izumi ◽  
Masanori Kishino
Keyword(s):  
Ionic Liquid ◽  
Enzymatic Saccharification ◽  
Cellulose Regeneration

scholarly journals Sequential enzymatic saccharification and fermentation of ionic liquid and organosolv pretreated agave bagasse for ethanol production

2017 ◽  
Vol 225 ◽  
pp. 191-198 ◽  
Author(s):  
Jose A. Pérez-Pimienta ◽  
Alejandra Vargas-Tah ◽  
Karla M. López-Ortega ◽  
Yessenia N. Medina-López ◽  
Jorge A. Mendoza-Pérez ◽  
...  
Keyword(s):  
Ionic Liquid ◽  
Ethanol Production ◽  
Enzymatic Saccharification

Ionic Liquid 1-Butyl-3-Methylimidazolium Chloride/FeCl3 Pretreatment of Sugarcane Residue for Second Generation Bioethanol Production

2013 ◽  
Vol 275-277 ◽  
pp. 1662-1665 ◽  
Author(s):  
Qiang Li ◽  
Juan Juan Fei ◽  
Xu Ding Gu ◽  
Geng Sheng Ji ◽  
Yang Liu ◽  
...  
Keyword(s):  
Ionic Liquid ◽  
Enzymatic Hydrolysis ◽  
Low Temperature ◽  
Second Generation ◽  
Reducing Sugars ◽  
Enzymatic Saccharification ◽  
Cellulosic Biomass ◽  
Candida Shehatae ◽  
Second Generation Bioethanol ◽  
Temperature Pretreatment

This study aims to establish a natural cellulosic biomass pretreatment process using ionic liquid (IL) for efficient enzymatic hydrolysis and second generation bioethanol. The IL 1-Butyl-3-methylimidazolium Chloride/FeCl3 ([Bmim]Cl/FeCl3) was selected in view of its low temperature pretreatment ability and the potential of accelerating enzymatic hydrolysis, and it could be recyclable. The yield of reducing sugars from sugarcane residue pretreated with this IL at 80 oC for 1 h reached 46.8% after being enzymatically hydrolyzed for 24 h. Sugarcane residue regenerated were hydrolyzed more easily than that treated with water. The fermentability of the hydrolyzates, obtained after enzymatic saccharification of the regenerated sugarcane residue, was transformed into bioethanol using Candida shehatae. This microbe could absorb glucose and xylose efficiently, and the ethanol production was 0.38 g/g glucose within 30 h fermentation. In conclusion, the metal ionic liquid pretreatment in low temperature shows promise as pretreatment solvent for natural biomass.

scholarly journals One-Pot Ionic Liquid-Mediated Bioprocess for Pretreatment and Enzymatic Hydrolysis of Rice Straw with Ionic Liquid-Tolerance Bacterial Cellulase

2022 ◽  
Vol 9 (1) ◽  
pp. 17
Author(s):  
Malinee Sriariyanun ◽  
Nichaphat Kitiborwornkul ◽  
Prapakorn Tantayotai ◽  
Kittipong Rattanaporn ◽  
Pau-Loke Show
Keyword(s):  
Ionic Liquid ◽  
Saline Soil ◽  
Enzymatic Saccharification ◽  
Value Added ◽  
Specific Enzyme ◽  
One Pot ◽  
The One ◽  
Hydrolysis Of ◽  
Bacterial Cellulase ◽  
Value Added Products

Ionic liquid (IL) pretreatment of lignocellulose is an efficient method for the enhancement of enzymatic saccharification. However, the remaining residues of ILs deactivate cellulase, therefore making intensive biomass washing after pretreatment necessary. This study aimed to develop the one-pot process combining IL pretreatment and enzymatic saccharification by using low-toxic choline acetate ([Ch][OAc]) and IL-tolerant bacterial cellulases. Crude cellulases produced from saline soil inhabited Bacillus sp. CBD2 and Brevibacillus sp. CBD3 were tested under the influence of 0.5–2.0 M [Ch][OAc], which showed that their activities retained at more than 95%. However, [Ch][OAc] had toxicity to CBD2 and CBD3 cultures, in which only 32.85% and 12.88% were alive at 0.5 M [Ch][OAc]. Based on the specific enzyme activities, the sugar amounts produced from one-pot processes using 1 mg of CBD2 and CBD3 were higher than that of Celluclast 1.5 L by 2.0 and 4.5 times, respectively, suggesting their potential for further application in the biorefining process of value-added products.

scholarly journals Comparison of dilute acid and ionic liquid pretreatment of switchgrass: Biomass recalcitrance, delignification and enzymatic saccharification

2010 ◽  
Vol 101 (13) ◽  
pp. 4900-4906 ◽  
Author(s):  
Chenlin Li ◽  
Bernhard Knierim ◽  
Chithra Manisseri ◽  
Rohit Arora ◽  
Henrik V. Scheller ◽  
...  
Keyword(s):  
Ionic Liquid ◽  
Enzymatic Saccharification ◽  
Dilute Acid ◽  
Biomass Recalcitrance ◽  
Ionic Liquid Pretreatment

In Situ Saccharification of Cellulose in Mild Ionic Liquid Using Sodium Alginate Immobilized Cellulase

2013 ◽  
Vol 361-363 ◽  
pp. 339-342 ◽  
Author(s):  
Juan Juan Fei ◽  
Qiang Li ◽  
Yuan Yuan Feng ◽  
Geng Sheng Ji ◽  
Xu Ding Gu ◽  
...  
Keyword(s):  
Ionic Liquid ◽  
Sodium Alginate ◽  
Reducing Sugars ◽  
Enzymatic Saccharification ◽  
Reducing Sugar ◽  
Candida Shehatae ◽  
Ionic Liquid Treatment ◽  
Immobilized Cellulase

The work is to select biocompatible ionic liquid (IL) toward in situ saccharification of cellulose and investigating the effect of enzymatic saccharification with sodium alginate immobilized cellulase. The [Mmi [DM was selected for the ionic liquid treatment improved the yield of reducing sugars and the hydrolyzates could be efficiently fermented to ethanol. The yield of reducing sugar is 89.54% for 48h. In the in situ saccharification process, the yield of sugars were 84.52% and 86.72% with immobilized cellulase and free cellulase saccharification for 48h. Then the hydrolyzates could be fermented to ethanol withCandida shehatae. The yield of ethanol was 0.42g/g glucose within 24h.

Comparison of ionic liquid, acid and alkali pretreatments for sugarcane bagasse enzymatic saccharification

2011 ◽  
Vol 86 (10) ◽  
pp. 1342-1348 ◽  
Author(s):  
Li Wan Yoon ◽  
Gek Cheng Ngoh ◽  
Adeline Seak May Chua ◽  
Mohd. Ali Hashim
Keyword(s):  
Ionic Liquid ◽  
Sugarcane Bagasse ◽  
Enzymatic Saccharification ◽  
Liquid Acid
Sign in / Sign up

Export Citation Format

Share Document