Chlamydia pneumoniae DNA in Peripheral Venous Blood Samples from Patients with Carotid Artery Stenosis

Objective: Previous studies have shown that the neuron-specific- enolase (NSE), S100B protein (S100B) and matrix metalloproteinase-9 (MMP9) are specific markers for studying cerebral injury. This study was aimed to demonstrate these biomarkers for their correlation with reperfusion after carotid artery stenting (CAS). Methods: In this study, a total of 44 patients who were diagnosed unilateral carotid artery stenosis by digital subtraction angiography (DSA) and underwent CAS, were selected as the operation groups. The patients' blood samples were collected at three different time points: T1, prior to operation; T2, next morning after operation (24 hours); T3, three days after operation (72 hours); All of the patients with the operation received computed tomography perfusion (CTP) at T1 and T3. The second group of 12 patients, who were excluded for carotid artery stenosis by DSA, were assigned to be the control group; Blood samples of these patients were collected at T1. The concentrations of NSE, S100B and MMP9 in serum from patients of both groups were detected by ELISA. Results: All of the operations were implanted in stents successfully without complications. (1) After CAS, rCBF increased while rMTT and rTTP decreased. (2) The concentrations of NSE, S100B and MMP9 in the serum decreased gradually (T1>T2>T3). There was no significant difference between the control group and the operation group at T1 (P>0.05) on their concentrations of NSE, S100B and MMP9 in the serum. When compared among the operation groups, the concentrations of NSE, S100B and MMP9 in the serum at T1 and T3 showed significant difference (P < 0.05). (3) Correlation analysis among the operation groups indicated that NSE, S100B, MMP9 and rCBF were positively correlated before operation (r = 0.69, 0.58 and 0.72, respectively, P < 0.05), as well as after operation (r = 0.75, 0.65 and 0.60, respectively, P < 0.05). Conclusion: We concluded that the concentrations of NSE, S100B and MMP9 in serum decreased with the improvement of cerebral reperfusion after CAS. NSE, S100B and MMP9 can be used as laboratory biochemical markers to evaluate the improvement of reperfusion after CAS. The results very well complement the imaging methods, such as CTP.

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Abstract TMP37: The Biomakers Expression Following Reperfusion After Carotid Artery Stenting

Stroke ◽

10.1161/str.51.suppl_1.tmp37 ◽

2020 ◽

Vol 51 (Suppl_1) ◽

Author(s):

Fuqiang Guo

Keyword(s):

Carotid Artery ◽

Carotid Artery Stenosis ◽

Carotid Artery Stenting ◽

Artery Stenosis ◽

Control Group ◽

Digital Subtraction ◽

Serum Concentrations ◽

Blood Samples ◽

Significant Difference ◽

Cerebral Reperfusion

Objective: This study was to demonstrate the biomarkers (NSE,MMP9,S100B,HO-1,CO) in serum for their correlation with reperfusion after carotid artery stenting (CAS). Methods: In this study, a total of 44 patients who were diagnosed with carotid artery stenosis (symptomatic/ asymptomatic stenosis≥70%) by Digital Subtraction Angiography (DSA) and underwent CAS successfully, were selected as the operation groups. The patients’ blood samples were collected at three different time points: T1, prior to operation; T2, next morning after operation (24 hours); T3, three days after operation (72 hours); The second group of 30 patients, who were excluded for carotid artery stenosis by DSA, were assigned to be the control group; Blood samples of these patients were collected at T1. The concentrations of NSE, S100B, MMP9, HO-1 and CO in serum from patients of both groups were detected by ELISA. Results: (1) There was no significant difference between the control group and the operation group at T1 (P>0.05) on their concentrations of NSE, S100B, MMP9, HO-1 and CO in the serum. (2) All of the operations were implanted in stents successfully without complications and the reperfusion improved after CAS. The concentrations of NSE, S100B, MMP9 and CO in the serum decreased gradually.(T1: 378.53±187.74MU/ml, 20.04±9.27ng/ml, 3.28±1.62ng/ml, 2.21±1.98umol/l respectively; T2: 302.65±160.46MU/ml, 16.45±8.95ng/ml, 2.81±1.63ng/ml, 1.49±1.10umol/l respectively, T1 vs T2: P<0.01; T3: 280.60±159.22MU/ml, 12.62±8.32ng/ml, 2.59±1.55ng/ml, 1.22±1.04umol/l respectively,T1 vs T3: P<0.01). (3) The serum concentrations of HO-1 in the T2 increased when compared with T1 (P<0.05) and obviously increased in the T3 when compared with T1 (P<0.01). Conclusions: We concluded that the concentrations of NSE, S100B, MMP9 and CO in serum decreased with the improvement of cerebral reperfusion after CAS. NSE, S100B, MMP9 and CO can be used as biomarkers to evaluate the improvement of reperfusion after CAS. What’s more, the changes of HO-1 after CAS may be associated with hyper-perfusion syndrome. Key words: NSE; S100B; MMP9; CO; HO-1;reperfusion; carotid artery stenting

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The presence of some cytokines and Chlamydia pneumoniae in the atherosclerotic carotid plaque in patients with carotid artery stenosis

Postępy Higieny i Medycyny Doświadczalnej ◽

10.5604/17322693.1140498 ◽

2015 ◽

Vol 69 ◽

pp. 227-232

Author(s):

Dariusz Janczak ◽

Piotr Ziolkowski ◽

Tomasz Szydełko ◽

Tadeusz Dorobisz ◽

Dawid Janczak ◽

...

Keyword(s):

Carotid Artery ◽

Carotid Artery Stenosis ◽

Carotid Plaque ◽

Chlamydia Pneumoniae ◽

Artery Stenosis

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Carotid artery stenosis detected by ultra-sonography-does correlate with anatomic findings R.-G. Ricardo, A. Jorge, V. Ricardo, M. Rolando. Hospital Universitario, U.A.N.L. Monterrey, N.L. Mexico

European Journal of Ultrasound ◽

10.1016/s0929-8266(97)90034-3 ◽

1997 ◽

Vol 5 ◽

pp. 13-14

Keyword(s):

Carotid Artery ◽

Carotid Artery Stenosis ◽

Artery Stenosis

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Methods for Improvement of Velocity Measurement for Carotid Artery Stenosis Estimation

European Journal of Ultrasound ◽

10.1016/s0929-8266(97)87277-1 ◽

1997 ◽

Vol 6 ◽

pp. S40

Author(s):

P Hoskins

Keyword(s):

Carotid Artery ◽

Carotid Artery Stenosis ◽

Velocity Measurement ◽

Artery Stenosis

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Oxidative injury associated with stenting of asymptomatic carotid artery stenosis

VASA ◽

10.1024/0301-1526/a000621 ◽

2017 ◽

Vol 46 (4) ◽

pp. 268-274

Author(s):

Erhan Saraçoğlu ◽

Ertan Vuruşkan ◽

Yusuf Çekici ◽

Salih Kiliç ◽

Halil Ay ◽

...

Keyword(s):

Oxidative Stress ◽

Carotid Artery ◽

Oxidative Damage ◽

Carotid Artery Stenosis ◽

Artery Stenosis ◽

Oxidative Stress Marker ◽

Asymptomatic Carotid ◽

Imaging Methods ◽

Neurological Findings ◽

Asymptomatic Carotid Artery Stenosis

Abstract. Background: After carotid artery stenting (CAS), neurological complications that cannot be explained with imaging methods may develop. In our study we aimed to show, using oxidative stress markers, isolated oxidative damage and resulting neurological findings following CAS in patients with asymptomatic carotid artery stenosis. Patients and methods: We included 131 neurologically asymptomatic patients requiring CAS. The neurological findings were evaluated using the modified Rankin Scale (mRS) prior to the procedure, one hour post-procedure, and two days after. Patients with elevated mRS scores but with or without typical hyperintense lesions observed on an MRI and with changes of oxidative stress marker levels at the time (Δtotal-thiol, Δtotal antioxidative status [TAS], and Δtotal oxidant status [TOS]) were evaluated. Results: In the neurological examination carried out one hour prior to the procedure, there were 92 patients with mRS = 0, 20 with mRS = 1, and 12 with mRS = 2. When Δtotal-thiol, ΔTAS, and ΔTOS values and the mRS were compared, it was observed that as the difference in oxidative parameters increased, clinical deterioration also increased proportionally (p = 0.001). Conclusions: We demonstrate a possible correlation between oxidative damage and neurological findings after CAS which could not be explained by routine imaging methods.

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