Automated liquid culture system misses isoniazid heteroresistance in Mycobacterium tuberculosis isolates with mutations in the promoter region of the inhA gene

2014 ◽  
Vol 34 (3) ◽  
pp. 555-560 ◽  
Author(s):  
Z. Zhang ◽  
J. Lu ◽  
Y. Wang ◽  
Y. Pang ◽  
Y. Zhao
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Promoter Region ◽  
Culture System ◽  
Liquid Culture ◽  
Liquid Culture System

Expression of the naphthoate synthase gene in Mycobacterium tuberculosis in a self-generated oxygen depleted liquid culture system

Anaerobe ◽  
2010 ◽  
Vol 16 (6) ◽  
pp. 610-613 ◽  
Author(s):  
Prathna Ramchandra ◽  
A. Willem Sturm
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Culture System ◽  
Liquid Culture ◽  
Liquid Culture System

scholarly journals Rifampin Resistance Missed in Automated Liquid Culture System for Mycobacterium tuberculosis Isolates with Specific rpoB Mutations

2013 ◽  
Vol 51 (8) ◽  
pp. 2641-2645 ◽  
Author(s):  
L. Rigouts ◽  
M. Gumusboga ◽  
W. B. de Rijk ◽  
E. Nduwamahoro ◽  
C. Uwizeye ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Culture System ◽  
Liquid Culture ◽  
Liquid Culture System ◽  
Rifampin Resistance

scholarly journals Comparison of MGIT and Myco/F Lytic Liquid-Based Blood Culture Systems for Recovery of Mycobacterium tuberculosis from Pleural Fluid

2015 ◽  
Vol 53 (4) ◽  
pp. 1391-1394 ◽  
Author(s):  
Elizabeth Harausz ◽  
John Kafuluma Lusiba ◽  
Mary Nsereko ◽  
John L. Johnson ◽  
Zahra Toossi ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Pleural Fluid ◽  
Culture System ◽  
Liquid Culture ◽  
Content Type ◽  
Indicator Tube ◽  
Liquid Culture System ◽  
Mycobacterial Growth ◽  
Mycobacterial Growth Indicator Tube ◽  
Growth Indicator

The specificities and sensitivities of the Bactec mycobacterial growth indicator tube (MGIT) system for the recovery ofMycobacterium tuberculosisfrom pleural fluid are not statistically different than those of the Myco/F lytic liquid culture system. The time to positivity is shorter in the MGIT system (12.7 versus 20.7 days, respectively;P= 0.007). The Myco/F lytic culture system may be an alternative to the MGIT system for diagnosing pleural tuberculosis.

scholarly journals A liquid culture system for murine megakaryocyte progenitor cells

Blood ◽  
1982 ◽  
Vol 59 (2) ◽  
pp. 250-257
Author(s):  
T Nagasawa ◽  
M Nakazawa ◽  
T Abe
Keyword(s):  
Progenitor Cells ◽  
Conditioned Medium ◽  
Culture System ◽  
Liquid Culture ◽  
Culture Medium ◽  
Pokeweed Mitogen ◽  
Plasma Clot ◽  
Liquid Culture System ◽  
Cell Conditioned Medium ◽  
Number Of Cells

A liquid culture system is described for murine megakaryocyte progenitor cells (CFU-M) in the presence of pokeweed-mitogen-stimulated spleen-cell conditioned medium. There were dose-related responses between the number of CFU-M developed and the number of cells cultured and the dosage of conditioned medium in this liquid culture system. Murine CFU-M were abundantly cloned in this system an the plating efficiency was similar in comparison with that in a plasma clot system. The acetylcholinesterase-positive colonies (more than 4 acetylcholinesterase-positive cells) were clearly seen on day 3 of culture, and they reached a maximum (60.5 +/- 10.7/2 x 10(5) cells) on day 7 of culture. Ultrastructural analyses of megakaryocytic maturation in this system showed that a few megakaryocytes produced platelets that were released in the culture medium on day 5 of culture. This liquid culture system is suitable for the study of the dynamic process of the megakaryocyte-platelet system.

scholarly journals Effect of gangliosides on murine megakaryocytopoiesis in a liquid culture system.

1988 ◽  
Vol 13 (4) ◽  
pp. 293-300
Author(s):  
Yasuko Watanabe ◽  
Masami Taniguchi ◽  
Nobuko Fukamachi ◽  
Bonro Kobayashi
Keyword(s):  
Culture System ◽  
Liquid Culture ◽  
Liquid Culture System
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