Application of abscisic acid (S-ABA) at different stages of ripening on color development of ‘Rubi’ table grape

The color of the berries is an important aspect of the quality of table grapes and crucial for marketing. The ‘Rubi’ table grapes grown in the subtropical climate generally lack color intensity due to the inhibition of anthocyanins by high temperatures during ripening. The exogenous application of abscisic acid (S-ABA) can be used to overcome this problem as the accumulation of anthocyanins in the berry skin is regulated by this plant growth regulator. The objective of this study was to assess the effect of the exogenous application of S-ABA at different stages of ripening on color development in ‘Rubi’ table grapes using the soluble solids (SS) content as a marker of ripening. The study was conducted during two seasons in commercial vineyards. The first trial was conducted in Marialva, Parana, Brazil, during the 2019 summer season crop (harvest in December). S-ABA (400 mg L-1) was exogenously applied at different stages of ripening of ‘Rubi’ table grapes (determined by the SS content of the berries): control (without application); SS = 8-9 ºBrix; SS = 10-11 ºBrix; SS = 8-9 ºBrix (two applications; the second one applied 10 days after the first); and SS = 10-11 ºBrix (two applications; the second one applied 10 days after the first). The second trial was conducted in Cambira, Parana, Brazil, during the 2020 off-season crop (harvest in May). S-ABA (400 mg L-1) was exogenously applied at different stages of ripening of ‘Rubi’ grapes: control (without application); SS = 6-7 ºBrix; SS = 7-8 ºBrix; SS = 9-10 ºBrix; SS = 6-7 ºBrix (two applications; the second one applied 14 days after the first); SS = 7-8 ºBrix (two applications; the second one applied 14 days after the first); and SS = 9-10 ºBrix (two applications; the second one applied 14 days after the first). A randomized block design was used as the statistical model with four replications, and each plot consisted of one vine. The variables analyzed were total anthocyanin contents, color index (CIRG), and color attributes (L*, C*, h°, and ΔE) of berry skin. The total anthocyanin accumulation and color attributes of the berries were evaluated every 10 and 7 days after the first application of S-ABA until harvest in the first and second trials, respectively, and the other variables were evaluated at harvest. In the summer-season crop, when the SS content was 8-11 ºBrix, the application of S-ABA increased the concentration of the total anthocyanins 4 times compared to that in the control, improving berry color development. Furthermore, in the off-season crop, when the SS content was 6-10 ºBrix, the application of S-ABA increased the concentration of total anthocyanins 2-3 times compared to that in the control, improving the color attributes of berries. In both crops, a single application of the plant growth regulator was sufficient to intensify the color of the berries.

Self-assembly of 1-triacontanol onto Layered Doubled Hydroxide Nano-carrier toward Sustainable Growth Regulation of Maize

1-triacontanol has attracted considerable research interest in plant growth regulator field, owing to its physiological effect in seed germination, root growth and photosynthesis enhancement. In this work, a 1-triacontanol modified...

Aplikasi Zat Pengatur Tumbuh dengan Jumlah Daun Entres yang Berbeda terhadap Keberhasilan Sambung Pucuk Durian (Application of Plant Growth Regulator with Different Number of Scion Leaves on Success of Durian Top Grafting)

<p>Perbanyakan tanaman secara vegetatif menggunakan teknik sambung pucuk dapat digunakan sebagai alternatif untuk menghasilkan benih yang bermutu. Namun, masalah yang sering terjadi pada sambung pucuk adalah kegagalan sambung. Penelitian ini bertujuan untuk mendapatkan jenis zat pengatur tumbuh, jumlah daun entres, dan kombinasi perlakuan yang memberikan hasil terbaik terhadap keberhasilan sambung pucuk durian. Penelitian dilaksanakan pada bulan November 2018 sampai Januari 2019 di Desa Alasmalang, Kemranjen, Banyumas. Percobaan yang dilakukan merupakan percobaan pot dengan rancangan faktorial. Perlakuan pada penelitian ini adalah kombinasi antara zat pengatur tumbuh (kontrol, ekstrak tauge, air kelapa, IBA, dan BAP) dan jumlah daun entres (2, 4, dan 6 helai). Rancangan penelitian yang digunakan yaitu Rancangan Acak Kelompok Lengkap dengan 15 perlakuan dan diulang tiga kali. Hasil penelitian menunjukkan perlakuan ZPT IBA dan BAP memberikan pengaruh nyata terhadap jumlah tunas sambung pucuk tanaman durian, yaitu sebesar 2,711 dan 2,822 dan perlakuan jumlah daun entres dua helai memberikan pengaruh yang nyata terhadap waktu pecah tunas, jumlah tunas, dan pertambahan jumlah daun sambung pucuk tanaman durian, yaitu sebesar 2,3 helai.</p><p><strong>Keywords</strong></p><p>Sambung pucuk durian; Daun entres; Zat pengatur tumbuh</p><p><strong>Abstract</strong></p><p>Vegetative propagation of plants using shoot grafting can be used as an alternative to produce quality seeds. The problem that often occours in top grafting is the failure to graft. This experiment aims to obtain the type of plant growth regulator, the number of scion’s leaves, and the combination that give the best results for growth of top grafting in durian. The research was conducted in November 2018 to January 2019 in Alasmalang Village, Kemranjen, Banyumas. The experiment was a pot experiment with a factorial design. The treatment was a combination of growth regulator (control, bean extract, coconut water, IBA, and BAP) and number of scion leaves (2, 4, and 6 strands). The research design used was a RCBD with 15 treatments and three replications. The results showed that the treatment of PGR IBA and BAP had a significant effect on the number of grafted shoots of durian plants, namely 2.711 and 2.822 and the treatment of the number of leaves of two leaves had a significant effect on shoot break time, the number of shoots and the increase in the number of grafted leaves of durian plants, namely amounting to 2.3 strands.</p>

Determination of biological properties of the new synthetic plant growth regulator - spyrocarbon complex with boric acid by means of phytotests

Determining the environmental safety of new synthetic plant growth regulators is an urgent problem. Spirocarbon derivatives were subjected to such determination by means of a specially developed system based on phytotests "germinated seeds". This system allows the values of biometric, cytological and biochemical parameters of the phytotest to determine the toxic, cytotoxic, genotoxic (mutagenic) effects and induction of molecular stress by the synthetic chemical substance being tested. This system was used to establish the biological properties of a new drug - a complex of spirocarbon with boric acid - in relation to five phytotests monocotyledons and dicotyledons plants. Phytotesting was performed in the spectrum of concentrations (10-7-10-2 mol/dm3) of this drug. It demonstrated either the absence or presence of a weak toxic effect on phytotests, which did not exceed 30%. The study showed that the plant growth regulator spirocarbon in combination with boric acid has growthregulating properties, the manifestation of which depends on the concentration of the drug and the species characteristics of plants. Only the maximum concentration (10-2 моль/дм3) of the tested drug is slightly toxic to plants. The obtained results testify to the biological efficiency and ecological safety of the synthesized drug. This allows us to recommend it for use in agricultural practice.

The effectiveness of the use of Energia-M growth regulator in the protection system of winter wheat

In the formation of the winter wheat yield, the protection of the crop from pests, stress, pesticides and adverse abiotic factors is one of the important elements of the technology. To increase yield and reduce the impact of adverse factors in winter wheat protection systems, plant growth regulators are used. In the conditions of the Ryazan region in 2019-2020, the effectiveness of the use of tank mixtures of pesticides with the growth regulator Energia-M (0.01 kg/ha) in the protection systems of winter wheat of the Danaya variety was studied. In the variant with the use of a tank mixture of pesticides with a growth regulator, a statistically significant excess of yield was noted in comparison with the control (without treatment) by 1.4 t/ha (31 %) and with the variant with treatment using a tank mixture without the use of a growth regulator - by 0.9 t/ha (18 %). Under the influence of the plant growth regulator, a decrease in the negative impact of pesticides on growth processes, an increase in crop yield by 18 % due to the formation of an additional number of productive stems (80 pcs / m2 ) were noted and a higher net operating profit was obtained compared to the protection system without a plant growth regulator.

Effects of cultivar, seed rate, N rate and plant growth regulator on key processing quality parameters of winter and spring-sown oats

The effects of three seeding rates (250, 350 and 450 seeds/m2), six applied N rates (40, 70, 100, 130, 160 and 190 kg N/ha) and the application of plant growth regulator on the grain quality of two spring oat cultivars (Husky and Keely) were evaluated at four spring-sown sites over a 3-year period (2016–2018). The same cultivars, seeding rates and five applied N rates (80, 110, 140, 170 and 200 kg N/ha) were evaluated at three winter-sown sites, in 2017–2019. Keely had a significantly higher kernel content (KC) in spring (0.7%) and winter-sown (0.6%) oats. Specific weight (SW) decreased as N rate increased in spring-sown crops (1 kg/hl) with no effect observed in winter-sown oats. Hullability (HB) increased (0.92%) in spring-sown crops as N rate increased with a reduction (0.9%) observed when winter-sown. Increases in grain protein percentage (GPP) were observed in spring (1.8%) and winter (1.2%) sowings in response to N rate. The application of plant growth regulator reduced SW (0.4 kg/hl), GPP (0.3%), KC (0.6%) with no effect on HB. Kernel size was highly responsive too applied N rate, with reductions in grains >2.5 mm and increases in grains of smaller size observed as N rate increased. HB had a marked relationship to kernel size with GP inconsistently linked to SW. The results of this study suggest that cultivar, N rate and PGR application are of importance to milling quality and that changes in grain size in response to agronomic practices contribute to changes in specific weight and hullability.

An ABA Functional Analogue B2 Enhanced Salt Tolerance by Inducing the Root Elongation and Reducing Peroxidation Damage in Maize Seedlings

Salt stress negatively affects maize growth and yield. Application of plant growth regulator is an effective way to improve crop salt tolerance, therefore reducing yield loss by salt stress. Here, we used a novel plant growth regulator B2, which is a functional analogue of ABA. With the aim to determine whether B2 alleviates salt stress on maize, we studied its function under hydroponic conditions. When the second leaf was fully developed, it was pretreated with 100 µM ABA, 0.01 µM B2, 0.1 µM B2, and 1 µM B2, independently. After 5 days treatment, NaCl was added into the nutrient solution for salt stress. Our results showed that B2 could enhance salt tolerance in maize, especially when the concentration was 1.0 µMol·L−1. Exogenous application of B2 significantly enhanced root growth, and the root/shoot ratio increased by 7.6% after 6 days treatment under salt stress. Compared with control, the ABA level also decreased by 31% after 6 days, which might have resulted in the root development. What is more, B2 maintained higher photosynthetic capacity in maize leaves under salt stress conditions and increased the activity of antioxidant enzymes and decreased the generation rate of reactive oxygen species by 16.48%. On the other hand, B2 can enhance its water absorption ability by increasing the expression of aquaporin genes ZmPIP1-1 and ZmPIP1-5. In conclusion, the novel plant growth regulator B2 can effectively improve the salt tolerance in maize.

Transformation of Zinnia elegans Jacq. as an ornamental potted plant by daminozide application

Zinnia elegans Jacq. is one of the ornamental plants potential to be used as a potted ornamental plants. The problem to be resolved is the size of the plant can reach 1 m, so it is necessary to modify the plant height into 20 cm to 25 cm using retardant (plant growth regulator), called daminozide. The purpose of this study was to determine the best concentration and soaking time using daminozide to inhibit the growth of zinnia. The research was conducted at Mangkuyudan 57, Yogyakarta. This research was arranged in a factorial design with 3 blocks as replication. Different concentrations of daminozide were used as first factor with three levels (1 g.L-1, 2 g.L-1, and 3 g.L-1) and soaking times were used as the second factor (12 h, 24 h, and 36 h). The data were analyzed using analysis of variance and continued with HSD-Tukey at the α = 5 %. The results showed that there were an interaction between daminozide concentration and soaking time in the height of Z. elegans. There were also positive correlation between plant height, number of flower, and flowering period. The best combination of daminozide concentration and soaking times were 2 g.L-1 and 12 h. This treatment gave the best height that fit to the criteria of a potted plant which was 20.08 cm. However, it reduced flower’s diameter, number of flowers, and canopy’s size.