Optimisation of gentiopicroside production in Gentiana kurroo Royle from adventitious root cultures in a liquid culture system

Author(s):  
Mariadoss Alphonse ◽  
Kalaivani Thiagarajan
2019 ◽  
Vol 137 (1) ◽  
pp. 101-113 ◽  
Author(s):  
Nguyen Thi Nhat Linh ◽  
Le Kim Cuong ◽  
Ho Thanh Tam ◽  
Hoang Thanh Tung ◽  
Vu Quoc Luan ◽  
...  

Blood ◽  
1982 ◽  
Vol 59 (2) ◽  
pp. 250-257
Author(s):  
T Nagasawa ◽  
M Nakazawa ◽  
T Abe

A liquid culture system is described for murine megakaryocyte progenitor cells (CFU-M) in the presence of pokeweed-mitogen-stimulated spleen-cell conditioned medium. There were dose-related responses between the number of CFU-M developed and the number of cells cultured and the dosage of conditioned medium in this liquid culture system. Murine CFU-M were abundantly cloned in this system an the plating efficiency was similar in comparison with that in a plasma clot system. The acetylcholinesterase-positive colonies (more than 4 acetylcholinesterase-positive cells) were clearly seen on day 3 of culture, and they reached a maximum (60.5 +/- 10.7/2 x 10(5) cells) on day 7 of culture. Ultrastructural analyses of megakaryocytic maturation in this system showed that a few megakaryocytes produced platelets that were released in the culture medium on day 5 of culture. This liquid culture system is suitable for the study of the dynamic process of the megakaryocyte-platelet system.


1988 ◽  
Vol 13 (4) ◽  
pp. 293-300
Author(s):  
Yasuko Watanabe ◽  
Masami Taniguchi ◽  
Nobuko Fukamachi ◽  
Bonro Kobayashi

2012 ◽  
Vol 11 (2) ◽  
pp. 173-179 ◽  
Author(s):  
Asuka Uchida ◽  
Akiha Abe ◽  
Yoichiro Hoshino ◽  
Hisato Kunitake

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